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Neurobehavioural changes and morphological study of cerebellar purkinje cells in kaolin induced hydrocephalus

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Abstract

Cerebellar abnormalities are commonly associated with hydrocephalus. However, the effect of hydrocephalus on the otherwise normal cerebellum has been largely neglected. This study assesses the morphological changes in the Purkinje cells in relation to cerebellar dysfunction observed in juvenile hydrocephalic rats. Fifty-five three-week old albino Wistar rats were used, hydrocephalus was induced by intracisternal injection of kaolin (n = 35) and others served as controls (n = 20). Body weight measurements, hanging wire, negative geotaxis, and open field tests were carried out at the onset and then weekly for 4 weeks, rats were killed, and their cerebella processed for Hematoxylin and Eosin, Cresyl violet and Golgi staining. Qualitative and quantitative studies were carried out; quantitative data were analyzed using two-way ANOVA and independent T tests at p < 0.05. Hydrocephalic rats weighed less than controls (p = 0.0247) but their cerebellar weights were comparable. The hydrocephalic rats had a consistently shorter latency to fall in the hanging wire test (F(4,112) = 18.63; p < 0.0001), longer latency to turn in the negative geotaxis test (F(4,112) = 22.2; p < 0.0001), and decreased horizontal (F(4,112) = 4.172, p = 0.0035) and vertical movements (F(4,112) = 4.397; p = 0.0024) in the open field test than controls throughout the 4 weeks post-induction. Cellular compression in the granular layer, swelling of Purkinje cells with vacuolations, reduced dendritic arborization and increased number of pyknotic Purkinje cells were observed in hydrocephalic rats. Hydrocephalus caused functional and morphological changes in the cerebellar cortex. Purkinje cell loss, a major pathological feature of hydrocephalus, may be responsible for some of the motor deficits observed in this condition.

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Authors and Affiliations

  1. Department of Anatomy, College of Medicine, University of Ibadan, PO Box 200284, Ibadan, Nigeria

    Funmilayo Eniola Olopade, Omowumi Femi-Akinlosotu, Adejoke Joan Adekanmbi, Seun Ajani & Matthew Temitayo Shokunbi

  2. Department of Surgery, College of Medicine, University of Ibadan, Ibadan, Nigeria

    Matthew Temitayo Shokunbi

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  1. Funmilayo Eniola Olopade
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  2. Omowumi Femi-Akinlosotu
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  5. Matthew Temitayo Shokunbi
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Correspondence to Matthew Temitayo Shokunbi.

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Olopade, F.E., Femi-Akinlosotu, O., Adekanmbi, A.J. et al. Neurobehavioural changes and morphological study of cerebellar purkinje cells in kaolin induced hydrocephalus. Anat Sci Int 96, 87–96 (2021). https://doi.org/10.1007/s12565-020-00561-z

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