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Molecular cloning and expression analysis of common carp Cyprinus carpio tumor necrosis factor-α

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Abstract

Tumor necrosis factor (TNF) is a pleiotropic and potent inflammatory cytokine produced during inflammation. In this study, we cloned the tumor necrosis factor-α (TNFα) complementary DNA (cDNA) gene from common carp Cyprinus carpio. The TNFα4 cDNA was 1318 bp in length and contained a 768-bp open reading frame, which putatively encoded 255 amino acids. The cloned sequences have several polymorphisms in the 3′ untranslated regions. The TNFα putative protein retains the TNFα family signature and consists of a four-exon, three-intron structure similar to other known TNFα4 genes. Aeromonas hydrophila Ahcs01 induced expression of TNFα4 in carp; differential expression was observed at different times. Our results indicate that TNFα4 is a central regulatory and effector cytokine of carp inflammatory and antimicrobial responses.

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References

  1. Cerami A (1992) Inflammatory cytokines. Clin Immunol Immunopathol 62:S3–S10

    Article  PubMed  CAS  Google Scholar 

  2. Hirono I, Nam BH, Kurobe T, Aoki T (2000) Molecular cloning, characterization, and expression of TNF cDNA and gene from Japanese flounder Paralychthys olivaceus. J Immunol 165:4423–4427

    PubMed  CAS  Google Scholar 

  3. Zou J, Wang T, Hirono I, Aoki T, Inagawa H, Honda T et al (2002) Differential expression of two tumor necrosis factor genes in rainbow trout, Oncorhynchus mykiss. Dev Comp Immunol 26:161–172

    Article  PubMed  CAS  Google Scholar 

  4. Saeij JP, Stet RJM, de Vries BJ, van Muiswinkel WB, Wiegertjes GF (2003) Molecular and functional characterization of carp TNF: a link between TNF polymorphism and trypanotolerance. Dev Comp Immunol 27:29–41

    Article  PubMed  CAS  Google Scholar 

  5. Savan R, Sakai M (2004) Presence of multiple isoforms of TNF alpha in carp (Cyprinus carpio L.): genomic and expression analysis. Fish Shellfish Immunol 17:87–94

    Article  PubMed  CAS  Google Scholar 

  6. Zou J, Secombes CJ, Long S, Miller N, Clem LW, Chinchar VG (2003) Molecular identification and expression analysis of tumornecrosis factor in channel catfish (Ictalurus punctatus). Dev Comp Immunol 27:845–858

    Article  PubMed  CAS  Google Scholar 

  7. Garcia-Castillo J, Chaves-Pozo E, Olivares P, Pelegrin P, Meseguer J, Mulero M (2004) The tumor necrosis factor a of the bony fish seabream exhibits the in vivo proinflammatory and proliferative activities of its mammalian counterparts, yet it functions in a species-specific manner. Cell Mol Life Sci 61:1331–1340

    Article  PubMed  CAS  Google Scholar 

  8. Garcia-Castillo J, Pelegrin P, Mulero V, Meseguer J (2002) Molecular cloning and expression analysis of tumor necrosis factor a from a marine fish reveal its constitutive expression and ubiquitous nature. Immunogenetics 54:2000–2007

    Google Scholar 

  9. Ordas MC, Costa MM, Roca FJ, Lopez-Castejon G, Mulero V, Meseguer J et al (2007) Turbot TNFα gene: molecular characterization and biological activity of the recombinant protein. Mol Immunol 44:389–400

    Article  PubMed  CAS  Google Scholar 

  10. Forlenza M, Magez S, Scharsack JP, Westphal A, Westphal A, Savelkoul HFJ, Wiegertjes GF (2009) Receptor-mediated and lectin-like activities of carp (Cyprinus carpio) TNF-α. J Immunol 183:5319–5332

    Article  PubMed  CAS  Google Scholar 

  11. Roca FJ, Mulero I, López-Muñoz A, Sepulcre MP, Renshaw SA, Meseguer J, Mulero V (2008) Evolution of the inflammatory response in vertebrates: fish TNF-α is a powerful activator of endothelial cells but hardly activates phagocytes. J Immunol 181:5071–5081

    PubMed  CAS  Google Scholar 

  12. Basu M, Swain B, Sahoo BR, Maiti NK, Samanta M (2012) Induction of toll-like receptor (TLR) 2, and MyD88-dependent TLR- signaling in response to ligand stimulation and bacterial infections in the Indian major carp, mrigal (Cirrhinus mrigala). Mol Biol Rep 39:6015–6028

    Article  PubMed  CAS  Google Scholar 

  13. Basu M, Swain B, Maiti NK, Routray P, Samanta M (2012) Inductive expression of toll-like receptor 5 (TLR5) and associated downstream signaling molecules following ligand exposure and bacterial infection in the Indian major carp, mrigal (Cirrhinus mrigala). Fish Shellfish Immunol 32:121–131

    Article  PubMed  CAS  Google Scholar 

  14. Apweiler R, Attwood TK, Bairoch A, Bateman A, Birney E, Biswas M, Bucher P, Cerutti L, Corpet F, Croning MD, Durbin R, Falquet L, Fleischmann W, Gouzy J, Hermjakob H, Hulo N, Jonassen I, Kahn D, Kanapin A, Karavidopoulou Y, Lopez R, Marx B, Mulder NJ, Oinn TM, Pagni M, Servant F (2001) The InterPro database, an integrated documentation resource for protein families, domains and functional sites. Nucleic Acids Res 29:37–40

    Article  PubMed  CAS  Google Scholar 

  15. Hofmann K, Stoffel W (1993) TMbase—a database of membrane spanning protein segments. Biol Chem Hoppe Seyler 374:166

    Google Scholar 

  16. Sitnikova T, Rzhetsky A, Nei M (1995) Interior-branch and bootstrap tests of phylogenetic trees. Mol Biol Evol 12:319–333

    PubMed  CAS  Google Scholar 

  17. Nylund A, Devold M, Plarre H, Isdal E, Asrseth M (2003) Emergence and maintenance of infectious salmon anaemia virus (ISAV) in Europe: a new hypothesis. Dis Aquat Org 56:11–24

    Article  PubMed  CAS  Google Scholar 

  18. Skall HF, Olesen NJ, Mellergaard S (2005) Viral haemorrhagic septicaemia virus in marine fish and its implications for fish farming-a review. J Fish Dis 28:509–529

    Article  PubMed  CAS  Google Scholar 

  19. Wang XW, Ao JQ, Li QG, Chen XH (2006) Quantitative detection of a marine fish iridovirus isolated from large yellow croaker, Pseudosciaena crocea, using a molecular beacon. J Virol Methods 133:76–81

    Article  PubMed  CAS  Google Scholar 

  20. Aoki T (1992) Chemotherapy and drug resistance in fish farms in Japan. In: Shariff M, Subasighe RP, Arthur JR (eds) Diseases in Asian aquaculture. Fish Health Section, Manila. Asian Fisheries Society, Philippines, pp 519–529

  21. Secombes CJ, Wang T, Hong S, Peddie S, Crampe M, Laing KJ, Cunningham C, Zou J (2001) Cytokines and innate immunity of fish. Dev Comp Immunol 25:713–723

    Google Scholar 

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Acknowledgments

This work was supported by grants from the National Natural Science Foundation of China (30571433, 30972277).

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Correspondence to Qiang Lu.

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Zhao, X., Duan, D., Feng, X. et al. Molecular cloning and expression analysis of common carp Cyprinus carpio tumor necrosis factor-α. Fish Sci 78, 1229–1236 (2012). https://doi.org/10.1007/s12562-012-0563-8

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  • DOI: https://doi.org/10.1007/s12562-012-0563-8

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