Skip to main content
SpringerLink
Log in

Effects of ultrasonic treatment on collagen extraction from skins of the sea bass Lateolabrax japonicus

  • Original Article
  • Food Science and Technology
  • Published:
Fisheries Science Aims and scope Submit manuscript

Abstract

In this study we investigated the effects of ultrasonic wave treatment on the extraction yield of acid-soluble collagen from sea bass skins. Two extraction methods were compared: a 24 h acid treatment using 0.5 M acetic acid (1:200 sample/acid, w/v) and an extraction using ultrasonic treatment after the addition of a 0.5 M acetic acid solution. The results indicated that the extraction yield of collagen increased with the ultrasonic treatment, with the extraction rate increasing rapidly at higher amplitudes of ultrasonic treatment. The subunit compositions of the collagen extracted by ultrasonic treatment were analyzed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis, which revealed that the α1(α3), α2, and β chains of collagen were present early in the ultrasonic treatment. An unknown component, believed to be a product of collagen degradation induced by the ultrasonic treatment, was detected only after a longer treatment time. The component extracted by the ultrasonic treatment was determined to be collagen based on the finding that there were no changes in the main components of collagen, specifically, the α1(α3), α2, and β chains, following pepsin treatment.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4
Fig. 5

Similar content being viewed by others

References

  1. Cavallaro JF, Kemp PD, Kraus KH (1994) Collagen fabrics as biomaterials. Biotechnol Bioeng 43:781–791

    Article  PubMed  CAS  Google Scholar 

  2. Togashi SI, Takahashi N, Iwama M, Watanabe S, Tamagawa K, Tukui T (2002) Antioxidative collagen-derived peptides in human-placenta extract. Placenta 23:497–502

    Article  PubMed  CAS  Google Scholar 

  3. Samonina G, Ashmarin I, Lyapina L (2002) Glyproline peptide family: review on bioactivity and possible origins. Pathophysiology 8:229–234

    Article  PubMed  CAS  Google Scholar 

  4. Ashimarin IP, Karazeeva EP, Lyapina LA, Samonia GE (1988) The simplest proline-containing peptide PG, GP, PGP and GPGG: regulatory activity and possible sources of biosynthesis. Biochemistry (Moscow) 63:119–124

    Google Scholar 

  5. Lyapina LA, Pastorava VE, Samonia GE, Ashimarin IP (2000) The effect of PGP peptide and PGP-rich substances on haemostatic parameters of rat blood. Blood Coagul Fibrinolysis 11:1–6

    Article  Google Scholar 

  6. Samonia GE, Kopyliva GN, German SV, Umarova BA, Bakaeva ZV, Zeliaznik NJ. Zhuykova SE, Sergeev VI, Lukjanzeva GV, Smirnova EA, Lelekova TV. Endogenous peptides and gastric mucosal homeostasis. In: Pathophysiology of organs and system. Typical physiological processes (experimental and clinical aspects). Proc 2nd Russian Congress of Photophysiology. Moscow, p 133

  7. Gudasheva TA, Boyko SS, Akparov VKH, Ostroskaya RU, Scoldinov SP, Rozantsev GG, Votonia TA, Zherdev VP, Seredenin SV (1996) Identification of a novel endogenous memory facilitation cyclic dipeptide cyclo-proly in rat brain. FEBS Lett 391:149–152

    Article  PubMed  CAS  Google Scholar 

  8. Ciarlo A, Paredi M, Fraga A (1997) Isolation of soluble collagen from hake skin (Merluccius hubbsi). J Aquat Food Prod Tech 6:65–77

    Article  CAS  Google Scholar 

  9. Kruus P, O’Neill M, Robertson D (1990) Ultrasonic initiation of polymerization. Ultrasonics 28:304–309

    Article  CAS  Google Scholar 

  10. Ana CS, Mar V (2010) Effect of ultrasound on the technological properties and bioactivity of food: a review. Trends Food Sci Technol 21:323–331

    Article  Google Scholar 

  11. Kandos N, Lhche JL (2001) Sonochemistry of carbohydrate compounds. Carbohydr Res 332:115–131

    Article  Google Scholar 

  12. McClements D (1995) Advances in the application of ultrasound in food analysis and processing. Trends Food Sci Technol 6:293–299

    Article  CAS  Google Scholar 

  13. Shin HJ, Lee DC (2003) Study on the process to decrease the molecular weight of β-(1,6)-branched β-(1,3)-d-glucans. Korean J Biotechnol Bioeng 18:352–355

    Google Scholar 

  14. Kim SM, Park SM, Choi HM, Lee KT (1999) Optimal processing parameters of low of low molecular weight carrageenan by ultrasound. J Korean Fish Soc 32:495–500

    CAS  Google Scholar 

  15. Lee BS, Ko MS, Kim HJ, Kwak IS, Kim DH, Chung BW (2006) Separation of 6-gingerol from ginger and antioxidative activity. Korean J Biotechnol Bioeng 21:484–488

    Google Scholar 

  16. Association of Official Analytical Chemists (AOAC) (1999) In: Helrich K (ed) Official methods analysis, 14th edn. AOAC, Washington D.C.

  17. Gornall AG, Bardawill CJ, David MM (1949) Determination of serum proteins by means of the biuret reaction. J Biol Chem 177:751–766

    PubMed  CAS  Google Scholar 

  18. Laemmli UK (1970) Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227:680–685

    Article  PubMed  CAS  Google Scholar 

Download references

Acknowledgments

This study was supported by the research fund from Korea Research Institute, Korea.

Author information

Authors and Affiliations

  1. Korea Food Research Institute, 516 Baekhyundong, Bundanggu, Songnamsi Kyounggido, 466-746, Korea

    Hyun Kyung Kim, Young Ho Kim, Yun Ji Kim & Nam Hyouck Lee

  2. College of Life Science and Biotechnology, Korea University, Anamdong, Seongbukgu, Seoul, 136-701, Korea

    Hyun Jin Park

Authors
  1. Hyun Kyung Kim
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Young Ho Kim
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Yun Ji Kim
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Hyun Jin Park
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Nam Hyouck Lee
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Nam Hyouck Lee.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Kim, H.K., Kim, Y.H., Kim, Y.J. et al. Effects of ultrasonic treatment on collagen extraction from skins of the sea bass Lateolabrax japonicus . Fish Sci 78, 485–490 (2012). https://doi.org/10.1007/s12562-012-0472-x

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s12562-012-0472-x

Keywords

Search

Navigation