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Molecular cloning, characterization and expression analysis of a chymotrypsin-like serine protease from kuruma shrimp Marsupenaeus japonicus

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Abstract

The mRNA encoding chymotrypsin-like serine protease (Mj-chy) from a kuruma shrimp Marsupenaeus japonicus hepatopancreas was identified as a peptidoglycan-inducible gene by 5′-end serial analysis of gene expression. The transcript of Mj-chy consists of a 816-nucleotide open reading frame encoding 271 amino acids, including a signal peptide of 17 amino acids and a trypsin-like serine protease domain of 219 amino acids. Like most serine proteases, Mj-chy has a catalytic triad consisting of histidine, aspartic acid and serine, and six cysteine residues forming three disulfide bridges. In a phylogenetic analysis, the trypsin-like serine protease domain clustered with invertebrate chymotrypsins and was closely related to chymotrypsin-like serine protease from Chinese shrimp Fenneropenaeus chinensis and chymotrypsin BI from Pacific white shrimp Litopenaeus vannamei. Mj-chy was detected in the hepatopancreas, stomach and intestine, and exhibited increased expression in defense-related tissues (i.e., hemocytes, lymphoid organ and hepatopancreas) after peptidoglycan stimulation.

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Acknowledgments

This study was supported in part by grants from the Ministry of Agriculture, Forestry, and Fisheries of Japan and Grants in-Aid for Scientific Research from the Ministry of Education, Culture, Sports, Science and Technology of Japan. The authors especially thank Mr. Fernand F. Fagutao for proofreading the manuscript.

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Authors and Affiliations

  1. Laboratory of Genome Science, Tokyo University of Marine Science and Technology, Konan 4-5-7, Minato-ku, Tokyo, 108-8477, Japan

    Threechada Danwattananusorn, Hidehiro Kondo, Takashi Aoki & Ikuo Hirono

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  1. Threechada Danwattananusorn
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  2. Hidehiro Kondo
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  3. Takashi Aoki
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  4. Ikuo Hirono
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Correspondence to Ikuo Hirono.

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Danwattananusorn, T., Kondo, H., Aoki, T. et al. Molecular cloning, characterization and expression analysis of a chymotrypsin-like serine protease from kuruma shrimp Marsupenaeus japonicus . Fish Sci 75, 1231–1238 (2009). https://doi.org/10.1007/s12562-009-0159-0

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  • DOI: https://doi.org/10.1007/s12562-009-0159-0

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