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Evaluation of Immunomagnetic Separation Method for the Recovery of Hepatitis A Virus and GI.1 and GII.4 Norovirus Strains Seeded on Oyster and Mussel

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Abstract

Outbreaks of viral diseases are frequently associated with the consumption of minimally processed shellfish. Among the viruses in these outbreaks, hepatitis A virus (HAV) and human norovirus (NoV) have been increasingly reported as the most common food-borne pathogens. These viruses must be concentrated in tested samples in order to be detected. In this study, a method for the detection of NoV and HAV in shellfish using an immuno-magnetic separation (IMS) procedure combined with reverse transcriptase (RT)-PCR was developed. The IMS/RT-PCR method was applied to investigate the recovery rates of HAV, NoV GI.1, and GII.4 from oyster and mussel. Based on IMS/RT-PCR results, recovery rates for HAV from oyster and mussel test samples were 2.4 and 1.1 %, respectively. The NoV GI.1 recovery rates from oyster and mussel samples were 4.9–9.2 % (mean 6.9 %) and 4.3–8.6 % (mean 6.2 %), respectively, and the NoV GII.4 recovery rates were 8.8 and 8.5 %, respectively. These results verified that HAV, NoV GI.1, and GII.4 can be detected in all the test samples using the IMS/RT-PCR method, although the three inoculated viruses were recovered with low efficiency. In conclusion, the IMS/RT-PCR method can be used to efficiently and rapidly detect viruses such as HAV and NoV in shellfish such as oyster and mussel.

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Acknowledgments

This research was supported by the Fishery Commercialization Technology Development Program (#112089-3) of the Ministry for Food, Agriculture, Forestry, and Fisheries, Republic of Korea.

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Authors and Affiliations

  1. School of Food Science and Technology, Chung-Ang University, 4726 Seodongdaero, Ansung, Gyunggi-do, 456-756, Republic of Korea

    Ji-Hyoung Ha, Changsun Choi & Sang-Do Ha

  2. Department of Food Science and Technology, Chung-Ang University, 72-1 Nae-ri, Daeduk-myun, Ansung, Gyunggi-do, 456-756, Republic of Korea

    Sang-Do Ha

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  1. Ji-Hyoung Ha
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Correspondence to Sang-Do Ha.

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Ha, JH., Choi, C. & Ha, SD. Evaluation of Immunomagnetic Separation Method for the Recovery of Hepatitis A Virus and GI.1 and GII.4 Norovirus Strains Seeded on Oyster and Mussel. Food Environ Virol 6, 290–296 (2014). https://doi.org/10.1007/s12560-014-9156-2

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  • DOI: https://doi.org/10.1007/s12560-014-9156-2

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