Abstract
Dried Distiller’s Grains with Solubles (DDGS), a by-product of bio-ethanol production from maize and other cereals, is increasingly used as a feed additive. In this study, five Fusarium toxins, including fumonisin B1 (FB1), fumonisin B2 (FB2), deoxynivalenol (DON), zearalenone (ZEN) and beauvericin (BEA) were quantified by LC-MS/MS in 59 corn-DDGS samples. In addition, the fumonisin level in 30 randomly selected-samples was compared using an ELISA detection technique. No sample was free from mycotoxin contamination, and 50.8 % of the samples were co-contaminated with all five mycotoxins. Moreover, toxin levels were generally high, with mean levels of 9 mg kg−1 FB1, 6 mg kg−1 FB2, 1.2 mg kg−1 DON, 0.9 mg kg−1 ZEN, and 0.35 mg kg−1 BEA. Maximum levels for FB1 (143 mg kg−1) and FB2 (125 mg kg−1) are of acute toxicological relevance. The ELISA method had a tendency to underestimate the fumonisin content when compared with LC-MS/MS. Finally, this is the first reported beauvericin contamination in corn-DDGS.
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The project was partially financial supported by Kasertsart University Research and Development Institute (KURDI), Bangkok, Thailand.
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Tansakul, N., Jala, P., Laopiem, S. et al. Co-occurrence of five Fusarium toxins in corn-Dried Distiller’s Grains with Solubles in Thailand and comparison of ELISA and LC-MS/MS for fumonisin analysis. Mycotoxin Res 29, 255–260 (2013). https://doi.org/10.1007/s12550-013-0173-z
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DOI: https://doi.org/10.1007/s12550-013-0173-z