Abstract
Background
To describe mumps virus (MuV) used as a vector to express enhanced green fluorescent protein (EGFP) or red fluorescent protein (RFP) genes.
Methods
Molecular cloning technique was applied to establish the cDNA clones of recombinant mumps viruses (rMuVs). rMuVs were recovered based on our reverse genetic system of MuV-S79. The properties of rMuVs were determined by growth curve, plaque assay, fluorescent microscopy and determination of fluorescent intensity.
Results
Three recombinant viruses replicated well in Vero cells and similarly as parental rMuV-S79, expressed heterologous genes in high levels, and were genetically stable in at least 15 passages.
Conclusion
rMuV-S79 is a promising platform to accommodate foreign genes like marker genes, other antigens and immunomodulators for addressing various diseases.
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Funding
This work was supported partly by the Natural Science Foundation for Young Scholars of Zhejiang Province (LQ19H100005).
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Conceived and designed the experiments: ZZ and YW. Performed the experiments: DZ, MZ, YW, RL, XH, CZ, CQ, and DZ. Analyzed the data: DZ, YW, DZ, and ZZ. Wrote the manuscript: DZ, YW, MZ, ZZ, and all other co-authors edited the manuscript. All the authors approved the final manuscript as submitted and agree to be accountable for all aspects of the work.
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This study was approved by Children's Hospital, Zhejiang University School of Medicine (No. 2013119).
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The authors have no financial relationships relevant to this article to disclose.
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Zhou, D., Zhu, MY., Wang, YL. et al. Attenuated MuV-S79 as vector stably expressing foreign gene. World J Pediatr 15, 511–515 (2019). https://doi.org/10.1007/s12519-019-00287-7
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DOI: https://doi.org/10.1007/s12519-019-00287-7