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Knockdown expression of the B-type cyclin gene Orysa;CycB1;1 leads to triploid rice

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Abstract

B-type cyclins play key roles in the mitotic cell cycle and endoreduplication. Our previous study showed that knockdown of Orysa;CycB1;1 resulted in abnormal seeds and low seed production. To investigate the functions of B-type cyclins in plant reproductive growth, we analyzed the ploidy of the abnormal P ubi ::CycB1;1 RNAi embryo. The results showed that the abnormal P ubi ::CycB1;1 RNAi embryo was triploid. The triploid seedlings had larger reproductive organs than those of control plants, and were abortive. Semi-quantitative RT-PCR and in situ hybridization analyses showed that Orysa;CycB1;1 transcripts were localized in cells of anthers and ovaries. This indicated that Orysa;CycB1;1 is critical for reproductive growth and might be involved in gametogenesis in rice. The results of this study increase our understanding of the functions of B-type cyclins in rice reproductive growth.

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Authors and Affiliations

  1. College of Forestry, Shandong Agricultural University, Taian, Shandong, 271018, China

    Jing Guo

  2. State Key Laboratory of Crop Biology, College of Life Sciences, Shandong Agricultural University, Taian, Shandong, 271018, China

    Fang Wang & Xian Sheng Zhang

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  1. Jing Guo
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  2. Fang Wang
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  3. Xian Sheng Zhang
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Correspondence to Xian Sheng Zhang.

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Both authors contributed equally to this work

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Guo, J., Wang, F. & Zhang, X.S. Knockdown expression of the B-type cyclin gene Orysa;CycB1;1 leads to triploid rice. J. Plant Biol. 57, 43–47 (2014). https://doi.org/10.1007/s12374-013-0405-y

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  • DOI: https://doi.org/10.1007/s12374-013-0405-y

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