Abstract
Bamboo is one of the fastest growing plants in the world and is an economically important crop species in Asia. To identify the genes involved in fast shoot growth, an expressed sequence tag analysis was performed on Bambusa edulis Murno fast-growing shoots. Sequencing of the cDNA clones generated 1,402 5′-end high-quality expressed sequence tags (JG296384-JG297785, average length 655 bp), of which 1,101 clusters (143 consensus and 958 singletons) were revealed by sequence comparison to be unique and 597 (54% of total clusters) of them have a putative ATG start codon. A Basic Local Alignment Search Tool X analysis showed that 995 of these genes were similar to genes present in the National Center for Biotechnology database. A total of 868 genes were most similar to rice genes. The most abundant genes were three thionin-coding genes, which have 27, 17, or 10 clones, respectively, followed by aminocyclopropanecarboxylate oxidase and cysteine protease. Thionin and putative cell elongation-associated genes, xyloglucan endotransglycosylase/hydrolase, expansin, cellulose synthase, and pectin esterase were analyzed by real-time reverse transcription polymerase chain reaction using gene-specific primers. These results suggest that this high-quality library could be a good resource for understanding molecular events of bamboo shoot elongation, and the full-length clones could be used for crop improvement studies in the future.
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Abbreviations
- ACC:
-
Aminocyclopropanecarboxylate
- EST:
-
Expressed sequence tag
- NCBI:
-
National Center for Biotechnology
- XTH:
-
Xyloglucan endotransglycosylase/hydrolase
- UDPG:
-
Uridine diphosphate glucose
- GAP:
-
Glyceraldehyde 3-phosphate
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Acknowledgments
We thank Ms. Karen Bird, MSU-DOE Plant Research Laboratory, Michigan State University, East Lansing, for her help in editing our manuscript. This work was supported by a research grant from the Chungbuk National University in 2011.
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Eunjeong Cho and Yurry Um contributed equally to this work.
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Cho, E., Um, Y., Yoo, S.K. et al. An Expressed Sequence Tag Analysis for the Fast-Growing Shoots of Bambusa edulis Murno. J. Plant Biol. 54, 402–408 (2011). https://doi.org/10.1007/s12374-011-9179-2
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DOI: https://doi.org/10.1007/s12374-011-9179-2