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Rice Gene OsDSR-1 Promotes Lateral Root Development in Arabidopsis Under High-Potassium Conditions

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Abstract

Rice gene Oryza sativa Drought Stress Response-1 (OsDSR-1) was one of the genes identified to be responsive to drought stress in the panicle of rice at booting and heading stages by both microarray and quantitative real-time PCR analyses. OsDSR-1 encodes a putative calcium-binding protein, and its overexpression in Arabidopsis rendered transgenic plants to produce much shorter lateral roots (LRs) than wild-type (WT) plants in the medium supplemented with abscisic acid (ABA), suggesting that OsDSR-1 may act as a positive regulator during the process of ABA inhibition of LR development. No significant difference was observed in the total LR length between WT and transgenic plants in the media with the increase of only osmotic stress caused by NaCl, LiCl, and mannitol, while transgenic Arabidopsis seedlings appeared to produce larger root systems with longer total LR lengths under high-potassium conditions than WT seedlings. Further analysis showed that external Ca2+ was required for the production of larger root systems, indicating that the promotion by OsDSR-1 of the LR development of transgenic Arabidopsis seemed to occur in a Ca2+-dependent manner under high-potassium conditions. We propose that OsDSR-1 may function as a calcium sensor of the signal transduction pathway controlling the LR development under high-potassium conditions.

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References

  • Bao J, Chen FJ, Gu RL, Wang GY, Zhang FS, Mi GH (2007) Lateral root development of two Arabidopsis auxin transport mutants aux1-7 and eir1-1, in response to nitrate supplies. Plant Sci 173:417–425

    Article  CAS  Google Scholar 

  • Bologna G, Yvon C, Duvaud S, Veuthey AL (2004) N-terminal myristoylation predictions by ensembles of neural networks. Proteomics 4:1626–1632

    Article  PubMed  CAS  Google Scholar 

  • Boonburapong B, Buaboocha T (2007) Genome-wide identification and analyses of the rice calmodulin and related potential calcium sensor proteins. BMC Plant Biol 7:4

    Article  PubMed  Google Scholar 

  • Casimiro I, Beeckman T, Graham N, Bhalerao R, Zhang H, Casero P, Sandberg G, Bennett MJ (2003) Dissecting Arabidopsis lateral root development. Trends Plant Sci 8:165–171

    Article  PubMed  CAS  Google Scholar 

  • Chen WC, Yang YW, Lur HS, Tsai YG, Chang MC (2006) A novel function of abscisic acid in the regulation of rice (Oryza sativa L.) Root growth and development. Plant Cell Physiol 47:1–13

    Article  PubMed  Google Scholar 

  • Cheng SH, Willmann MR, Chen HC, Sheen J (2002) Calcium signaling through protein kinases: the Arabidopsis calcium-dependent protein kinase gene family. Plant Physiol 129:469–485

    Article  PubMed  CAS  Google Scholar 

  • Clough SJ, Bent AF (1998) Floraldip: a simplified method for Agrobacterium-mediated transformationof Arabidopsis thaliana. Plant J 16:735–743

    Article  PubMed  CAS  Google Scholar 

  • Cutler SR, Rodriguez PL, Finkelstein RR, Abrams SR (2010) Abscisic acid: emergence of a core signaling network. Annu Rev Plant Biol 61:651–679

    Article  PubMed  CAS  Google Scholar 

  • Deak KI, Malamy J (2005) Osmotic regulation of root system architecture. Plant J 43:17–28

    Article  PubMed  CAS  Google Scholar 

  • Delk NA, Johnson KA, Chowdhury NI, Braam J (2005) CML24, regulated in expression by diverse stimuli, encodes a potential Ca2+ sensor that functions in response to abscisic acid, daylength, and ion stress. Plant Physiol 139:240–253

    Article  PubMed  CAS  Google Scholar 

  • Emanuelsson O, Nielson H, Brunak S, Heijine GV (2000) Predicting subcelluar localization of proteins based on their N-terminal amino acid sequence. J Mol Biol 300:1005–1016

    Article  PubMed  CAS  Google Scholar 

  • Finkelstein R, Reeves W, Ariizumi T, Steber C (2008) Molecular aspects of seed dormancy. Annu Rev Plant Biol 59:387–415

    Article  PubMed  CAS  Google Scholar 

  • Guo Y, Xiong LM, Song CP, Gong DM, Halfter U, Zhu JK (2002) A calcium sensor and its interacting protein kinase are global regulators of abscisic acid signaling. Dev Cell 3:233–244

    Article  PubMed  CAS  Google Scholar 

  • Harrison SJ, Mott EK, Parsley K, Aspinall S, Gray JC, Cottage A (2006) A rapid and robust method of identifying transformed Arabidopsis thaliana seedlings following floral dip transformation. Plant Methods 2:19

    Article  PubMed  Google Scholar 

  • Kaplan B, Davydov O, Knight H, Galon Y, Knight MR, Fluhr R, Fromm H (2006) Rapid transcriptome changes induced by cytosolic Ca2+ transients reveal ABRE-related sequences as Ca2+-responsive cis elements in Arabidopsis. Plant Cell 18:2733–2748

    Article  PubMed  CAS  Google Scholar 

  • Kariola T, Brader G, Helenius E, Li J, Heino P, Palva ET (2006) Early responsive to dehydration 15, a negative regulator of abscisic acid responses in Arabidopsis. Plant Physiol 142:1559–1573

    Article  PubMed  CAS  Google Scholar 

  • Kim TH, Bohmer M, Hu H, Nishimura N, Schroeder JI (2010) Guard cell signal transduction network: advances in understanding abscisic acid, CO2, and Ca2+ signaling. Annu Rev Plant Biol 61:561–591

    Article  PubMed  CAS  Google Scholar 

  • Kutz A, Müller A, Hennig P, Kaiser WM, Piotrowski M, Weiler EW (2002) A role for nitrilase 3 in the regulation of root morphology in sulphur-starving Arabidopsis thaliana. Plant J 30:95–106

    Article  PubMed  CAS  Google Scholar 

  • Linkohr BI, Williamson LC, Fitter AH, Leyser HMO (2002) Nitrate and phosphate availability and distribution have different effects on root system architecture of Arabidopsis. Plant J 29:751–760

    Article  PubMed  CAS  Google Scholar 

  • López-Bucio J, Cruz-Ramirez A, Herrera-Estrella L (2003) The role of nutrient availability in regulating root architecture. Curr Opin Plant Biol 6:280–287

    Article  PubMed  Google Scholar 

  • Luan S, Kudla J, Rodriguez-Concepción M, Yalovsky S, Gruissem W (2002) Calmodulins and calcineurin B-like proteins: calcium sensors for specific signal response coupling in plants. Plant Cell 14(Suppl):S389–S400

    PubMed  CAS  Google Scholar 

  • McCormack E, Tsai YC, Braam J (2005) Handing calcium signaling: Arabidopsis CaMs and CMLs. Trends Plant Sci 10:383–389

    Article  PubMed  CAS  Google Scholar 

  • Phean-o-Pas S, Punteeanurak P, Buadboocha T (2005) Calcium signaling-mediated and differential induction of calmodulin gene expression by stress in Oryza sativa L. J Biochem Mol Biol 38:432–439

    PubMed  CAS  Google Scholar 

  • Shi HZ (2007) Integration of Ca2+ in plant drought and salt stress signal transduction pathways. In: Jenks MA, Hasegawa PM, Jain SM (eds) Advances in molecular breeding toward drought and salt tolerant crops. Springer, Berlin, Heidelberg, New York, pp 141–182

    Chapter  Google Scholar 

  • Smet ID, Signora L, Beeckman T, Inzé D, Foyer CH, Zhang H (2003) An abscisic acid-sensitive checkpoint in lateral root development of Arabidopsis. Plant J 33:543–555

    Article  PubMed  Google Scholar 

  • Snedden WA, Fromm H (2001) Calmodulin as a versatile calcium signal transducer in plants. New Phytol 151:35–66

    Article  CAS  Google Scholar 

  • Sάnchez-Calderόn L, Lόpez-Bucio J, Chacón-Lpóez A, Gutiérrez-Ortega A, Hernández-Abreu E, Herrera-Estrella L (2006) Characterization of low phosphorus insensitive mutants reveals a crosstalk between low phosphorus-induced determinate root development and the activation of genes involved in the adaptation of Arabidopsis to phosphorus deficiency. Plant Physiol 140:879–889

    Article  Google Scholar 

  • Verslues PE, Agarwal M, Katiyar-Agarwal S, Zhu JH, Zhu JK (2006) Methods and concepts in quantifying resistance to drought, salt and freezing, abiotic stresses that affect plant water status. Plant J 45:523–539

    Article  PubMed  CAS  Google Scholar 

  • White PJ, Broadley PJ (2003) Calcium in plant. Ann Bot 92(4):487–511

    Article  PubMed  CAS  Google Scholar 

  • Williamson LC, Ribrioux SP, Fitter AH, Ottoline Leyser HM (2001) Phosphate availability regulates root system architecture in Arabidopsis. Plant Physiol 126:875–882

    Article  PubMed  CAS  Google Scholar 

  • Xiong L, Schumaker KS, Zhu JK (2002) Cell signaling for cold, drought, and salt stresses. Plant Cell 14(suppl):S165–S183

    PubMed  CAS  Google Scholar 

  • Xiong LM, Wang RG, Mao GH, Koczan JM (2006) Identification of drought tolerance determinants by genetic analysis of root response to drought stress and abscisic acid. Plant Physiol 142:1065–1074

    Article  PubMed  CAS  Google Scholar 

  • Yang T, Poovaiah BW (2003) Calcium/calmodulin-mediated signal network in plants. Trends Plant Sci 8:505–512

    Article  PubMed  CAS  Google Scholar 

  • Zhu JK (2002) Salt and drought stress signal transduction in plants. Annu Rev Plant Biol 53:247–273

    Article  PubMed  CAS  Google Scholar 

Download references

Acknowledgments

This research was supported by One-Hundred Person Project of the Chinese Academy of Sciences (02200420062903) and the project of Nitrogen and Phosphorus Cycling and Manipulation for Agro-Ecosystems, the Knowledge Innovation Program of the Chinese Academy of Sciences (KZCX2-YW-T07)

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Authors and Affiliations

  1. Institute of Subtropical Agriculture, Chinese Academy of Sciences, Changsha, Hunan, 410125, People’s Republic of China

    Xinjie Xia

  2. Laboratory for Agro-ecological Process in Subtropical Region, Institute of Subtropical Agriculture, CAS, Changsha, Hunan, 410125, People’s Republic of China

    Xu Ming Yin, Pedro S. C. F. Rocha, Man Ling Wang, Yu Xin Zhu, Luo Ye Li, Shu Feng Song & Xinjie Xia

  3. Graduate School of the Chinese Academy of Sciences, Beijing, 100049, People’s Republic of China

    Xu Ming Yin & Shu Feng Song

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  1. Xu Ming Yin
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  2. Pedro S. C. F. Rocha
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  3. Man Ling Wang
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  4. Yu Xin Zhu
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  5. Luo Ye Li
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Correspondence to Xinjie Xia.

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Fig. 1

Screening of transgenic Arabidopsis plants by PCR using OsDSR-1-specific primers. M, DNA marker; line 1, wild type; lines 2–11, positive transgenic Arabidopsis (DOC 187 kb)

Fig. 2

Analysis of the expression level of OsDSR-1 in transgenic Arabidopsis by using reverse-transcription PCR with OsDSR-1 specific primers. M, DNA marker; line 1, wild type; lines 2–4, positive transgenic Arabidopsis (DOC 42 kb)

Fig. 3

Inhibition effect of LiCl on LR development. The total LR lengths of seedlings of Arabidopsis treated with different concentrations of LiCl (0, 5, and 10 mM) are shown. Data with different letters are significantly different at P = 0.05 (DOC 31 kb)

Fig. 4

Effects of NaCl and mannitol on LR development. The total LR lengths of Arabidopsis seedlings treated with different concentrations (0, 50, 75, and 100 mM) of NaCl (a) and mannitol (b) are shown. Data with different letters are significantly different at P = 0.05. The left panel represents data obtained from WT and transgenic Arabidopsis in the treatment media with CaCl2 (1.5 mM), and data obtained in the treatment media without CaCl2 are shown in the panel on the right (DOC 41 kb)

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Yin, X.M., Rocha, P.S.C.F., Wang, M.L. et al. Rice Gene OsDSR-1 Promotes Lateral Root Development in Arabidopsis Under High-Potassium Conditions. J. Plant Biol. 54, 180–189 (2011). https://doi.org/10.1007/s12374-011-9154-y

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  • DOI: https://doi.org/10.1007/s12374-011-9154-y

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