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Antioxidant Enzymes on Cell Membrane Integrity of Sugarcane Varieties Differing in Flooding Tolerance

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Abstract

Antioxidant system in terms of activity (superoxide dismutase [SOD], peroxidase [POX] and ascorbate peroxidase [APX]) and isozyme pattern (POX and SOD) in relation to cell membrane integrity was studied in different sugarcane varieties under flooding stress. Higher activity of POX, SOD and APX protects cells from oxidative damage and hence resistant types (Co 99006, Co 8371 and Co 99004) recorded lower malondialdehyde content (lipid peroxidation) compared to susceptible variety. A short term flooding stress induced over expression of the SOD and POX isoforms in tolerant types, while intensity of expression was comparatively less in susceptible type. These results suggested that the non specific POX, APX and SOD play a major role to control the reactive oxygen species and protect the cell from membrane damage induced during flooding stress.

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Abbreviations

SOD:

Superoxide dismutase

POX:

Peroxidase

APX:

Ascorbate peroxidase

MDA:

Malondialdehyde

NBT:

Nitroblue tetrazolium

TBA:

Thiobarbituric acid

ROS:

Reactive oxygen species

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Acknowledgments

We thank the Director, Sugarcane Breeding Institute, Coimbatore and Head, Division of Crop Production for providing facilities and support.

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Authors and Affiliations

  1. Department of Plant Physiology, Sugarcane Breeding Institute (ICAR), Coimbatore, 641007, Tamil Nadu, India

    Raju Gomathi, Gowri Manohari & Palaniappan Rakkiyappan

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  1. Raju Gomathi
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  2. Gowri Manohari
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  3. Palaniappan Rakkiyappan
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Correspondence to Raju Gomathi.

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Gomathi, R., Manohari, G. & Rakkiyappan, P. Antioxidant Enzymes on Cell Membrane Integrity of Sugarcane Varieties Differing in Flooding Tolerance. Sugar Tech 14, 261–265 (2012). https://doi.org/10.1007/s12355-012-0156-y

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