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Purification of a ribosome-inactivating protein with antioxidation and root developer potencies from Celosia plumosa

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Abstract

Considering Celosia plumosa as a potent antiviral plant, the attempt was made to determine, purify and characterize its proteinaceous antiviral elements against tobacco mosaic virus hypersensitive response on Nicotiana glutinosa. By using 60% ammonium sulphate-precipitation, FPLC-based anion and cation-exchange chromatography in 10 and 50 mM NaCl, size-exclusion chromatography in 50 mM NaCl and SDS–PAGE 10%, a 25 kD antiviral protein with ribosome-inactivating/28S rRNase ability was purified from the leaves of C. plumosa at vegetative growth stage. The purified protein showed FRAP-based antioxidant activity in vitro and caused 1.7-fold and 1.4-fold increases in the growth rate of root system upon carborundum-based application on the root growth medium of N. glutinosa. The present work reports an antiviral protein with ribosome-inactivating, antioxidation and root developer potencies in C. plumosa as an edible or ornamental plant that may be useful in health and agricultural biotechnology in the future.

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Abbreviations

FRAP:

Ferric reducing antioxidant power

AVP:

Antiviral proteins

RIP:

Ribosome-inactivating proteins

TMV:

Tobacco mosaic virus

BME:

Beta mercaptoethanol

PVP:

Polyvinylpyrrolidone

FPLC:

Fast performance liquid chromatography

SDS:

Sodium dodecyl sulphate

EGTA:

Ethylene glycol tetraacetic acid

DTT:

Dithiothreitol

BSA:

Bovine serum albumin

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Acknowledgements

The author of this paper is thankful to Iran National Science Foundation (INSF), Tehran and Research Institute for Fundamental Sciences (RIFS), University of Tabriz, Iran for their funding and providing facilities for the present research work.

Funding

Iran National Science Foundation, Tehran, Iran (Grant No. 88001730).

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Authors and Affiliations

  1. Iran National Science Foundation (INSF), Tehran, Iran

    Ashraf Gholizadeh

  2. Department of Biology, Research Institute for Fundamental Sciences (RIFS), University of Tabriz, Tabriz, Iran

    Ashraf Gholizadeh

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  1. Ashraf Gholizadeh
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Correspondence to Ashraf Gholizadeh.

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Gholizadeh, A. Purification of a ribosome-inactivating protein with antioxidation and root developer potencies from Celosia plumosa. Physiol Mol Biol Plants 25, 243–251 (2019). https://doi.org/10.1007/s12298-018-0577-5

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  • DOI: https://doi.org/10.1007/s12298-018-0577-5

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