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Optimization of in vitro regeneration of Haloxylon salicornicum: a keystone species of extreme arid regions

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Abstract

Haloxylon salicornicum is an economically important perennial woody shrub of family Amaranthaceae and is a good source of food additives, fodder, fuel and nutrients in Indian Thar Desert. In vitro regeneration of H. salicornicum through nodal shoot segments is achieved in present investigation. Young green shoots in horizontal orientation were found to be more responsive than vertical orientation. Axillary shoot buds were activated on MS medium containing 10 µM BAP. Adventitious roots with intense root hairs were induced on horizontally placed shoots on half strength MS medium containing 2 µM NOA + 100 mg L−1 activated charcoal. This is a first report on micropropagation of H. salicornicum. This protocol can be a useful means for in vitro characterization, mass propagation and conservation of the plant for future prospects.

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Acknowledgements

We are grateful to University Grants Commission (UGC), Government of India, for providing financial support through Major Research Project [F.No.39-411/2010 (SR)] and “Scheme for providing additional assistance to Universities” [F.No.4-1/2011 (SU)] to the Department of Botany, J N Vyas University, Jodhpur, India.

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  1. Plant Biotechnology and Molecular Biology Laboratory, Department of Botany, Jai Narain Vyas University, Jodhpur, India

    Deepak Krishna Vyas & Harchand R. Dagla

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  1. Deepak Krishna Vyas
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  2. Harchand R. Dagla
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Correspondence to Harchand R. Dagla.

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Vyas, D.K., Dagla, H.R. Optimization of in vitro regeneration of Haloxylon salicornicum: a keystone species of extreme arid regions. Physiol Mol Biol Plants 24, 1317–1321 (2018). https://doi.org/10.1007/s12298-018-0571-y

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  • DOI: https://doi.org/10.1007/s12298-018-0571-y

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