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PGPR regulate caspase-like activity, programmed cell death, and antioxidant enzyme activity in paddy under salinity

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Abstract

The response of two root associated bacteria Pseudomonas pseudoalcaligenes and Bacillus pumilus were studied in the (salt-sensitive) rice GJ17 cultivar to salinity under controlled environmental growth conditions for protection of plant from adverse effect of salinity. Salinity affects the growth of salt-sensitive cultivar, but inoculation of plant growth promoting rhizobacteria (PGPR) reduces the harmful effect of salinity. The present study states that PGPR helps to reduce lipid peroxidation and superoxide dismutase activity in salt-sensitive GJ17 cultivar under salinity and play an important role in the growth regulation for positive adaptation of plants to salt stress. This study shows that inoculation of paddy (Oryza sativa) with such bacteria could provide salt-tolerant ability by reducing the toxicity of reactive oxygen species by reducing plant cell membrane index, cell caspase-like protease activity, and programmed cell death and hence resulted in increase cell viability. As these isolates remain associated with the roots, the effects of tolerance against salinity are observed here. Results also indicate that isolated PGPR strain help in alleviating up to 1.5 % salinity stress as well as improve tolerance.

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Authors and Affiliations

  1. Department of Biotechnology N. V. Patel College of pure & Applied Sciences, S. P. University, V. V. Nagar, Anand, Gujarat, India

    Yachana Jha

  2. Department of Biosciences BRD School of Biosciences, Sardar Patel University, Post Box no. 39, V. V. Nagar, Anand, 388120, Gujarat, India

    R. B. Subramanian

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  1. Yachana Jha
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  2. R. B. Subramanian
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Correspondence to Yachana Jha.

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Jha, Y., Subramanian, R.B. PGPR regulate caspase-like activity, programmed cell death, and antioxidant enzyme activity in paddy under salinity. Physiol Mol Biol Plants 20, 201–207 (2014). https://doi.org/10.1007/s12298-014-0224-8

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  • DOI: https://doi.org/10.1007/s12298-014-0224-8

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