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In vitro micropropagation in Polygonatum verticillatum (L.) All. an important threatened medicinal herb of Northern India

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Abstract

An ideal micropropagation method of Polygonatum verticillatum has been developed using stem disc explants. Multiple shoots were initiated from stem disc explants on Murashige and Skoog (MS) medium fortified with different concentrations (0.25–10.0 mgl−1) and combinations of cytokinins (BAP, Kn and TDZ) along with (0.5–1.0 mgl−1) auxins (NAA/IBA/IAA). 1.0 mgl−1 BAP with 0.5 mgl−1 NAA was found to be the most effective in producing maximum number of shoots. Regular subculturing of these in vitro multiple shoots induced profuse growth of lateral roots in the same medium. Individual shoots were excised and rooted in vitro on half strength MS medium with 1.0 mgl−1 NAA. Regenerants were hardened in growth chamber with high humidity and showed a high rate of survival.

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Acknowledgements

We are thankful to University Grants Commission New Delhi for financial support to carry out the present work.

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Correspondence to Shivani Bisht.

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Bisht, S., Bisht, N.S. & Bhandari, S. In vitro micropropagation in Polygonatum verticillatum (L.) All. an important threatened medicinal herb of Northern India. Physiol Mol Biol Plants 18, 89–93 (2012). https://doi.org/10.1007/s12298-011-0091-5

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