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Increased puerarin biosynthesis during in vitro shoot formation in Pueraria tuberosa grown in growtek bioreactor with aeration

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Abstract

Puerarin accumulation during shoot cultures in static and liquid medium with or without aeration is described in Pueraria tuberosa (Roxb. ex Willd.) DC. Maximum shoot induction from nodal explants was achieved on Murashige and Skoog’s medium supplemented with 1.13 μM TDZ and 0.25 μM IBA after 4 weeks of growth. Puerarin content was higher in shoot cultures grown in liquid medium as compared to static medium. When shoots were grown in growtek bioreactor with different aeration volume, maximum puerarin content (1484 μg/g DW) was recorded with 20 % v/v aeration which was ~2.3 fold higher than puerarin content recorded in control cultures (cultures grown in growtek without aeration). Aeration requirement for organized (shoot) cultures was different for growth and puerarin accumulation.

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References

  • Ahmed S, Hahn EJ, Paek KY (2008) Aeration volume and photosynthetic photon flux affect cell growth and secondary metabolite contents in bioreactor cultures of Morinda citrifolia. J Plant Biol 51:209–212

    Article  CAS  Google Scholar 

  • Baldi A, Srivastava AK, Bisaria VS (2008) Effect of aeration on production of anticancer lignans by cell suspension cultures of Linum album. Appl Biochem Biotechnol 151:547–255

    Article  PubMed  CAS  Google Scholar 

  • Bhanja T, Rout S, Banerjee R, Bhattacharyya BC (2007) Comparative profiles of ά-amylase production in conventional tray reactor and growtek bioreactor. Bioprocess Biosyst Eng 30:369–376

    Article  PubMed  CAS  Google Scholar 

  • Choi YM, Jun HJ, Dawson K, Rodriguez RL, Roh MR, Jun J, Choi CH, Shim JH, Lee CH, Lee SJ, Park KH, Lee SJ (2010) Effect of the isoflavone puerarin and its glycosides on melanogenesis in B16 melanocytes. Eur Food Res Technol 231:75–83

    Article  CAS  Google Scholar 

  • Cui XH, Chakrabarty D, Lee EJ, Paek KY (2010) Production of adventitious roots and secondary metabolites by Hypericum perforatum L. in a bioreactor. Bioresour Technol 101:4708–16

    Article  PubMed  CAS  Google Scholar 

  • Goyal S, Ramawat KG (2007) Effect of chemical factors on production of isoflavonoids in Pueraria tuberosa (Roxb. ex Willd.) DC. suspension culture. Indian J Exp Biol 45:1063–1067

    PubMed  CAS  Google Scholar 

  • Goyal S, Ramawat KG (2008a) Synergistic effect of morphactin on cytokinin-induced production of isoflavonoids in cell cultures of Pueraria tuberosa (Roxb. ex Willd.) DC. Plant Growth Regul 55:175–181

    Article  CAS  Google Scholar 

  • Goyal S, Ramawat KG (2008b) Improvement of isoflavonoid accumulation by ethrel in cell suspension cultures of Pueraria tuberosa, a woody legume. Acta Physiol Plant 30:849–853

    Article  CAS  Google Scholar 

  • Luo CH, Yuan M, Chen MS, Liu SM, Ji H (2010) Metabolites of puerarin identified by liquid chromatography tandem mass spectrometry: Similar metabolic profiles in liver and intestine of rats. J Chromatogr B 878:363–370

    Article  CAS  Google Scholar 

  • Min JY, Jung HY, Kang SM, Kim YD, Kang YM, Park DJ, Prasad DT, Choi MS (2007) Production of tropane alkaloids by small-scale bubble column bioreactor cultures of Scopolia parviflora adventitious roots. Bioresour Tech 98:1748–1753

    Article  CAS  Google Scholar 

  • Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol Plant 15:473–497

    Article  CAS  Google Scholar 

  • Ramawat KG (2007) Production of alkaloids. In: Merillon JM, Ramawat KG (eds) Biotechnology: Secondary metabolites, 2nd edn. Science Publishers, Inc., Enfield, USA, pp 59–102

    Google Scholar 

  • Rathore MS, Shekhawat NS (2009) Micropropagation of Pueraria tuberosa (Roxb. Ex Willd.) and determination of puerarin content in different tissues. Plant Cell Tiss Organ Cult 99:327–334

    Article  CAS  Google Scholar 

  • Reppert A, Yousef GG, Rogers RB, Lila MA (2008) Isolation of radiolabeled isoflavones from Kudzu (Pueraria lobata) root cultures. J Agric Food Chem 56:7860–5

    Article  PubMed  CAS  Google Scholar 

  • Senoussi MM, Nora B, Joel C (2009) Impact of hypoxia on the growth and alkaloid accumulation in Catharanthus roseus cell suspension. Acta Physiol Plant 31:359–362

    Article  CAS  Google Scholar 

  • Sharma V, Goyal S, Ramawat KG (2009) Scale up production of isoflavonoids in cell suspension cultures of Pueraria tuberosa grown in shake flasks and bioreactor. Eng Life Sci 9:1–5

    Article  Google Scholar 

  • Shilpa K, Varun K, Laxhmi BS (2010) An alternate method of natural drug production: Eliciting secondary metabolite production using plant cell culture. J Plant Sci 5:222–247

    Article  Google Scholar 

  • Shinde AN, Malpathak N, Fulzele DP (2009) Induced high frequency shoenhanced isoflavones production in Psoralea corylifolia. Rec Nat Prod 3:38–45

    CAS  Google Scholar 

  • Teng Y, Cui H, Yang M, Song H, Zhang Q, Su Y, Zheng J (2009) Protective effect of puerarin on diabetic retinopathy in rats. Mol Biol Rep 36:1129–1133

    Article  PubMed  CAS  Google Scholar 

  • Vaishnav K, Goyal S, Ramawat KG (2006) Isoflavonoids production in callus culture of Pueraria tuberosa, the India kudzu. Indian J Exp Biol 44:1012–1017

    PubMed  CAS  Google Scholar 

  • Xu CM, Ou Y, Zhao B, Wang XD, Yuan XF, Wang YC (2008) Syringin production by Saussurea medusa cell cultures in a novel bioreactor. Biol Plant 52:377–380

    Article  CAS  Google Scholar 

  • Zhong JJ, Yoshida M, Fujiyama K, Seki T, Yoshida T (1993) Enhancement of anthocyanin production by Perilla frutescens cells in a stirred bioreactor with internal high irradiation. J Ferment Bioengr 75:299–303

    Article  CAS  Google Scholar 

  • Zhu LH, Wang L, Wang D, Jiang H, Tang QZ, Yan L, Bian ZY, Wang XA, Li H (2010) Puerarin attenuates high-glucose and diabetes- induced vascular smooth muscle cell proliferation by blocking PKCβ2/Rac 1-dependent signaling. Free Radic Biol Med 48:471–482

    Article  PubMed  CAS  Google Scholar 

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Acknowledgements

This work was supported by financial assistance from University Grants Commission-Departmental Research Programme (UGC-DRS) under special assistance program for medicinal plant research to K.G. Ramawat. V. Sharma and S. Goyal thanks UGC and Council of Scientific and Industrial Research (CSIR), New Delhi for financial assistance in the form of JRF and RA, respectively.

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Authors and Affiliations

  1. Laboratory of Bio-Molecular Technology, Department of Botany, M. L. Sukhadia University, Udaipur, 313001, India

    Varsha Sharma, Shaily Goyal & Kishan G. Ramawat

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  1. Varsha Sharma
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  2. Shaily Goyal
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  3. Kishan G. Ramawat
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Correspondence to Shaily Goyal.

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Sharma, V., Goyal, S. & Ramawat, K.G. Increased puerarin biosynthesis during in vitro shoot formation in Pueraria tuberosa grown in growtek bioreactor with aeration. Physiol Mol Biol Plants 17, 87–92 (2011). https://doi.org/10.1007/s12298-011-0049-7

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  • DOI: https://doi.org/10.1007/s12298-011-0049-7

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