Abstract
The extent of damage caused to the photosynthetic machinery of 10-d-old wheat seedlings by short-term exposure to mild heat, their capacity to recover from it and the possible roles of H2O2, SOD, catalase and ascorbate peroxidase on the recovery process were investigated. Seedlings were subjected to heat treatments at 40/42/44 °C for 20 min in the dark and allowed to grow for 72 h in light of different irradiances (40–800 μE m−2 s−1) at 20 °C for recovery from heat induced damage. Complete or partial recovery of photosynthetic activities was observed in the seedlings treated at 40 °C and 42 °C, but not at 44 °C. Our data suggest that the balance between (pro)oxidant and antioxidant levels poised by heat stress subsequent light is the crucial factor for the extent of recovery from heat induced damage.
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Abbreviations
- APx:
-
ascorbate peroxidase
- Chl:
-
chlorophyll
- PM:
-
photosynthetic machinery
- PSI:
-
photosystem I
- PSII:
-
photosystem II
- ROS:
-
reactive oxygen species
- SOD:
-
superoxide dismutase
- TBARs:
-
tiobarbituric acid reactive substances
References
Adir N, Zer H, Shochat S and Ohad I (2003) Photoinhibition — a historical perspective. Photosynth. Res., 76: 343–370.
Allakhverdiev SI, Kreslavski VD, Klimov VV, Los DA, Carpentier R and Mohanty P (2008) Heat stress: An overview of molecular responses in photosynthesis. Photosynth. Res., 98(1–3): 541–550.
Al-Khatib K and Paulsen GM (1989) Enhancement of thermal injury to photosynthesis in wheat plants and thylakoids by high light intensity. Plant Physiol., 90: 1041–1048.
Asada K (2006) Production and scavenging of reactive oxygen species in chloroplasts and their functions. Plant Physiol., 141: 391–396.
Berry JA and Björkman O (1980) Photosynthetic response and adaptation to temperature in higher plants. Annu Rev Plant Physiol., 31: 491–543.
Bukhov NG and Mohanty P (1999) Elevated temperature stress effects on photosystems: Characterization and evaluation of the nature of heat induced impairments. In: Concepts in photobiology: photosynthesis and photomorphogenesis (Eds. Singhal, G.S., Renger G., Sopory S.K., Irrgang K-D. and Govingjee ), New Delhi: Narosa Publishing House, pp. 617–648.
Dash S and Mohanty N (2001) Evaluation of assays for the analysis of thermo-tolerance and recovery potentials of seedlings of wheat (Triticum aestivum L.) cultivars. J. Plant Physiol., 158: 1153–1165.
Dash S and Mohanty N (2002) Response of seedlings to heat-stress in cultivars of wheat: Growth temperature-dependent differential modulation of photosystem 1 and 2 activity, and foliar antioxidant defense capacity. J. Plant Physiol., 159: 49–59.
Dhindsa RA and Matove W (1981) Drought tolerance in two mosses correlated with enzymatic defense against lipid peroxidation. J. Exp. Bot., 32: 79–91.
El-Shitinawy F, Ebrahim MKH, Sewelam N and El-Shourbagy MN (2004) Activity of photosystem 2, lipid peroxidation, and the enzymatic antioxidant protective system in heat shocked barley seedlings. Photosynthetica, 42: 15–21.
Giannopolitis N and Ries SK (1977) Superoxide dismutases I. Occurrence in higher plants. Plant Physiol., 59: 309–314.
Gong M, Chen B, Li ZG, Guo LH (2001) Heat-shock-induced cross adaptation to heat, chilling, drought and salt stress in maize seedlings and involvement of H2O2, J. Plant. Physiol. 158: 1125–1130.
Havaux M, Greppin H, Strasser RJ (1991) Functioning of photosystems I and II in pea leaves exposed to heat stress in the presence or absence of light. Planta 186:88–98.
Havaux M (1994) Temperature-dependent modulation of the photoinhibition-sensitivity of photosystem II in Solanum tuberosum leaves. Plant Cell Physiol. 35: 757–766.
Kadir S and Von Weihe M (2007) Photochemical efficiency and recovery of photosystem II in grapes after exposure to sudden and gradual heat stress. J. Amer. Soc. Hort. Sci., 132: 751–882.
Kreslavski V, Tatarinzev N, Shabnova N, Semenova G and Kosobryukhov A (2008) Characterization of the nature of photosynthetic recovery of wheat seedlings from short-time dark heat exposures and analysis of the mode of acclimation to different light intensities. J. Plant Physiol., 165: 1592–1600.
Kreslavski VD and Khristin MS (2003) Aftereffect of heat shock on fluorescence induction and low-temperature fluorescence spectra of wheat leaves. Russian J. Biophysics, 48: 806–813.
Laing WA, Greer DH and Schnell T (1995) Photoinhibition of photosynthesis causes a reduction in vegetative growth rates of dwarf bean (Phaseolus vulgaris) plants. Austral. J. Plant Physiol., 22: 511–520.
Larkindale J and Knight MR (2002) Protection against heat stress-induced oxidative damage in Arabidopsis involves calcium, abscisic acid, ethylene, and salicylic acid. Plant Physiol. 128: 682–695.
Lichtenthaler HK and Wellburn AR (1987) Chlorophylls and carotenoids: pigments of photosynthetic biomembranes. Methods Enzymol., 148: 350–382.
Maxwell K and Johnson GN (2002) Chlorophyll fluorescence: A practical guide. J. Exp. Bot. 345: 659–668.
Mittler R, Vanderauwera S, Gollery M and Van Breusegem F (2004) Reactive oxygen gene network of plants. Trends Plant Sci., 9: 490–498.
Murata N, Takanashi S, Nishiyama Y and Allakhverdiev SI (2007) Photoinhibition of photosystem II under environmental stress. Biochim. Biophys. Acta, 1767: 414–421.
Nakano J and Asada K (1981) Hydrogen peroxide is scavenged by ascorbate specific peroxidase in spinach chloroplasts Plant Cell Physiol., 22: 867–880.
Nishiyama Y, Yamamoto H, Allakhverdiev SI, Inaba M, Yokota A and Murata N (2001) Oxidative stress inhibits the repair of photodamage to the photosynthetic machinery. The EMBO J., 20: 5587–5594.
Paulsen, GM (1994) High temperature responses of crop plants. In: Physiology and determination of crop yield (Eds. Boote K.J et al.), ASA, CSSA, and SSSA, Madison, WI, pp. 365–389.
Sairam RK, Srivastava GC, Saxena DC (2000) Increased antioxidant activity under elevated temperatures: a mechanism of heat stress tolerance in wheat genotypes. Biol Plant 43: 245–251.
Sharkey TD (2005) Effects of moderate heat stress on photosynthesis: importance of thylakoid reactions, rubisco deactivation, reactive oxygen species, and thermotolerance provided by isoprene. Plant Cell Env., 28: 269–277.
Suzuki N and Mittler R (2006) Reactive oxygen species and temperature stresses: A delicate balance between signaling and destruction. Physiol. Plant., 126(1): 45–51.
Takahashi S and Murata N (2005) Interruption of the Calvin cycle inhibits the repair of photosystem II from photodamage Biochim. Biophys. Acta, 1708: 352–361.
Takahashi S, Nakamura T, Sakamizu M, van Woesik R and Yamasaki H (2004) Repair machinery of symbiotic photosynthesis as the primary target of heat stress for reef-building corals. Plant Cell Physiol., 45: 251–255.
Warm E and Laties GG (1982) Quantification of hydrogen peroxide in plant extracts by the chemiluminescence reaction with luminol. Phytochemistry, 21: 827–831
Yordanov I, Dilova S, Petkova R, Pangelova T, Goltsev V, Süss K.-H (1986) Mechanisms of the temperature damage and acclimation of the photosynthetic apparatus. Photobiochem. Photobiophys. 12: 147–155.
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Kreslavski, V.D., Lyubimov, V.Y., Shabnova, N.I. et al. Heat-induced impairments and recovery of photosynthetic machinery in wheat seedlings. Role of light and prooxidant-antioxidant balance. Physiol Mol Biol Plants 15, 115–122 (2009). https://doi.org/10.1007/s12298-009-0013-y
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DOI: https://doi.org/10.1007/s12298-009-0013-y