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Tissue specific response of Agrobacterium tumefaciens attachment to Sorghum bicolor (L) Moench

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Abstract

Agrobacterium mediated genetic transformation of plants have advantages over other methods, especially for making single copy transgenic plants with reduced chances of gene silencing and instability. However, monocotyledonous plant species could not utilize the full potential of this system because of possible limitations in Agrobacterium interaction with monocot plant cells. Agrobacterium attachment as a factor in genetic transformation was studied in the leaf, shoot apex, and leaf derived callus of sorghum (Sorghum bicolor (L) Moench). Pre-induction of Agrobacterium with acetosyringone was found necessary for Agrobacterium attachment to sorghum tissues. All the explants responded positively, with preferential Agrobacterium attachment and colonization around the tissues having actively dividing cells. Callus proved to be the best explant for Agrobacterium attachment as observed in scanning electron microscopy and transient GUS expression. Loss of Agrobacterium attachment was observed with an increase in the degree of tissue differentiation.

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Abbreviations

IAA:

Indole-3-acetic acid

IBA:

Indole-3-butyric acid

BAP:

Benzyl amino purine

ADS:

Adenine sulphate

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Author notes

  1. Anju Verma

    Present address: School of Biological Sciences, University of Missouri, Kansas City, MO, 64110, USA

Authors and Affiliations

  1. National Research Centre on Plant Biotechnology, IARI, New Delhi, 110012, India

    Anju Verma, Vikrant Nain, Chetana Kumari, Santosh Kumar Singh & P. Ananda Kumar

  2. School of Biotechnology, Institute of Post Graduate Studies and Research, Jawaharlal Nehru Technological University, Hyderabad, 500028, India

    M. Lakshmi Narasu

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  1. Anju Verma
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  2. Vikrant Nain
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  4. Santosh Kumar Singh
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  5. M. Lakshmi Narasu
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  6. P. Ananda Kumar
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Correspondence to P. Ananda Kumar.

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Verma, A., Nain, V., Kumari, C. et al. Tissue specific response of Agrobacterium tumefaciens attachment to Sorghum bicolor (L) Moench. Physiol Mol Biol Plants 14, 307–313 (2008). https://doi.org/10.1007/s12298-008-0028-9

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