Abstract
Arginase activity is important in polyamines and nitric oxide production which are required for the normal growth of placenta and embryo. A considerable arginase activity is observed in amniotic fluid in women at the end of pregnancy. Lecithin to sphingomyelin (L/S) ratio is widely used in order to assess fetal lung immaturity and prevention of neonatal respiratory distress syndrome, the major cause of neonatal morbidity and mortality. The purpose of our study was to determine if there is a relationship between arginase activity and L/S ratio in amniotic fluid. The study included 170 pregnant women, 18–43 years old, with normal and pathological pregnancy. The arginase activity was measured on the basis of the determination of the amount of liberated ornithine from arginine as substrate. The L/S ratio was done by using a thin layer chromatography. Increased level of arginase activity correlates with the fetal lung maturity. Arginase activity and L/S values may be useful biochemical data, for intrauterine baby maturity.
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Hui L, Meininger CJ, Hawker JR, Haynes TE, Kepka-Lenhart D, Mistry SK, et al. Regulatory role of arginase I and II in nitric oxide, polyamine, and proline synthesis in endothelial cells. Am J Physiol Endocrinol Metab. 2001;280(1):E75–82.
Cederbaum SD, Yu H, Grody WW, Kern RM, Yoo P, Iyer RK. Arginases I and II: do their functions overlap? Mol Genet Metab. 2004;81:S38–44.
Morris SM. Recent advances in arginine metabolism: roles and regulation of the arginase. Br J Pharmacol. 2009;157(6):922–30.
Durante W, Johnson FK, Johnson RA. Arginase: a critical regulator of nitric oxide synthesis and vascular function. Clin Exp Pharmacol Physiol. 2007;34(9):906–11. doi:10.1111/j.1440-1681.2007.04638.x.
Weiner CP, Knowles RG, Stegink LD, Dawson J, Moncada S. Myometrial arginase activity increases with advancing pregnancy in the guinea pig. Am J Obstet Gynecol. 1996;174(2):779–82.
Wu G, Bazer FW, Hu J, Johnson GA, Spencer TE. Polyamine synthesis from proline in the developing porcine placenta. Biol Reprod. 2005;72(4):842–50 Epub 2004 Dec 1.
Wu G, Bazer FW, Datta S, Johnson GA, Li P, Satterfield MC, Spencer TE. Proline metabolism in the conceptus: implications for fetal growth and development. Amino Acids. 2008;35(4):691–702 Epub 2008 Mar 11.
King MW. The medical biochemistry page. The extracellular matrix. © 1996–2013 themedicalbiochemistrypage.org, LLC | info @ themedicalbiochemistrypage.org.
Fozard JR, Part ML, Prakash NJ, Grove J, Schechter PJ, Sjoerdsma A, et al. l-Ornithine decarboxylase: an essential role in early mammalian embryogenesis. Science. 1980;208(4443):505–8. doi:10.1126/science.6768132.
Zhang C, Hein TW, Wang W, Chang C-I, Kuo L. Constitutive expression of arginase in microvascular endothelial cells counteracts nitric oxide-mediated vasodilatory function. FASEB J. 2001;. doi:10.1096/fj.00-0681fje.
Hirata M, Obayashi S, Sakamoto S, Aso T, Imamura M, Azuma H. Involvement of arginase in regulating myometrial contractions during gestation in the rat. Mol Hum Reprod. 2006;12(8):513–8.
Berkowitz DE, White R, Li D, Minhas KM, Cernetich A, Kim S, et al. Arginase reciprocally regulates nitric oxide synthase activity and contributes to endothelial dysfunction in aging blood vessels. Circulation. 2003;108:2000–6.
Skog S, Eriksson V, Eliasson E. Arginase, an S-phase enzyme in a human cell line. Biochim Biophys Acta. 1981;672(1):33–44.
Kropf P, Baud D, Marshall SE, Munder M, Mosley A, Fuentes JM, et al. Arginase activity mediates reversible T cell hyporesponsiveness in human pregnancy. Eur J Immunol. 2007;37(4):935–45.
Munder M. Arginase: an emerging key player in the mammalian immune system. British J Pharmacol. 2009;158:638–51.
Vincent D, Notter A, Magron J, Cellier-Chapuis C. Amniotic fluid arginase in women at term. C R Seances Soc Biol Fil. 1975;169(5):1232–6.
Yurdakök M. Inherited disorders of neonatal lung diseases. Turk J Pediatr. 2004;46(2):105–14.
Luo G, Norwitz ER. Revisiting amniocentesis for fetal lung maturity after weeks gestation. Rev Obstet Gynecol. 2008;1(2):61–8.
Whitsett JA, Weaver TE. Hydrophobic surfactant proteins in lung function and disease. N Engl J Med. 2002;347:2141–214.
Wijnberger LDE, de Kleine M, Voorbij HAM, Arabin B, Bruinse HW, Visser GHA, et al. Prediction of fetal lung immaturity using gestational age, patient characteristics and fetal lung maturity tests: a probabilistic approach. Arch Gynecol Obstet. 2010;281(1):15–21.
St Clair C, Norwitz ER, Woensdregt K, Cackovic M, Shaw JA, Malkus H, et al. The probability of neonatal respiratory distress syndrome as a function of gestational age and lecithin/sphingomyelin ratio. Am J Perinatol. 2008;25(8):473–80.
Gluck L, Kulovich MV, Borer RC Jr, Brenner PH, Anderson GG, Spellacy WN. Diagnosis of the respiratory distress syndrome by amniocentesis. Am J Obstet Gynecol. 1971;109(3):440–5.
Curet LB, Tsao FH, Zachman RD, Olson RW, Henderson PA. Phosphatidylglycerol, lecithin/sphingomyelin ratio and respiratory distress syndrome in diabetic and non-diabetic pregnancies. Int J Gynaecol Obstet. 1989;30(2):105–8.
Porembska Z, Kedra M. Early diagnosis of myocardial infarction by arginase activity determination. Clin Chim Acta. 1975;60(3):355–61.
Yip MCM, Knox WE. Function of arginase in lactating mammary gland. Biochem J. 1972;127(5):39–893.
Fox PF, Kelly AL. Indigenous enzymes in milk: overview and historical aspects-Part 1. Int Dairy J. 2006;16:500–16.
Remesar X, Arola L, Palou A, Alemany M. Arginase activity during pregnancy and lactation. Horm Metab Res. 1984;16(9):468–670.
Mori M. Regulation of nitric oxide synthesis and apoptosis by arginase and arginine recycling. J Nutr. 2007;137:1616S–20S.
Bansal RK, Goldsmith PC, He Y, Zaloudek CJ, Ecker JL, Riemer RK. A decline in myometrial nitric oxide synthase expression is associated with labor and delivery. J Clin Invest. 1997;99(10):2502–8.
Ali M, Buhimschi I, Chwalisz K, Garfield RE. Changes in expression of the nitric oxide synthase isoforms in rat uterus and cervix during pregnancy and parturition. Mol Hum Reprod. 1997;3(11):995–1003.
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Bjelakovic, G., Miladinovic, P., Jevtovic-Stoimenov, T. et al. Arginase Activity and Lecithin/Sphingomyelin (L/S) Ratio in the Amniotic Fluid of Pregnant Women. Ind J Clin Biochem 30, 84–88 (2015). https://doi.org/10.1007/s12291-013-0398-9
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DOI: https://doi.org/10.1007/s12291-013-0398-9