Abstract
Picrorhiza kurrooa is mentioned in Ayurveda for the treatment of many disorders, but it has not been subjected to systematic scientific investigations to assess its antidiabetic effect. The oral administration of aqueous and methanol extracts of P. kurrooa rhizomes (250 and 500 mg / kg body weight / day) for 15 days significantly reduced blood glucose, glycosylated haemoglobin and increased total hemoglobin, plasma insulin in alloxan-induced diabetes in albino rats. The treatment also showed significant correction in the level of nitric oxide radicals, superoxide radicals, peroxynitrite radical, lipid peroxidation, glutathione, glutathione reductase, glutathione-S-transferase, glutathione peroxidase, superoxide dismutase and catalase in the pancreas of alloxan diabetic rats.
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References
Ceriello A. Oxidative stress and glycemic regulation. Metabolism 2000; 49: 27–29.
Chandra A, Singh RK, Tewari L. Antioxidative potential of herbal hypoglycemic agents in diabetes — an overview. SFRR-India Bulletin 2004; 3: 24–26.
Chatterjee TK, editor. Herbal Options-Medicinal plants with antidiabetic properties, 3ed. India: Book and Allied (P) Ltd. Calcutta, 2000: 7–72.
Shukla R, Sharma SB, Puri D, Prabhu KM, Murthy PS. Medicinal plants for treatment of diabetes mellitus. Ind J Clin Biochem 2000; 15(Suppl.): 169–177.
Nadakarni KM, editor. The Indian Material Medica, vol. I, 3ed. India: Popular Book Depot, Dhoot Papeshwar, Bombay, 1954: 953–955.
Sochor M, Baquer NZ, McLean P. Induction of diabetes using single dose of alloxan monohydrate. Molecular Physiol 1985; 7: 51–55.
Cooper GR, McDaniell V, editors. Standard methods of clinical chemistry, edited by R. P. McDonald. New York: Academic Press, 1970: 159–164.
Drabkin DL, Austin JM. Spectrophotometric constants for common haemoglobin derivatives in human, dog and rabbit blood. J Biol Chem 1931; 98: 719–733.
Nayak SS, Pattabiraman TN. A new colorimetric method for the estimation of glycosylated hemoglobin. Clin Chem Acta 1981; 109: 267–274.
Yalow RS, Berson SA. Immunoassay of plasma insulin in man. Diabetes 1974; 10: 339–343.
Beutler E, Duran O, Kelley BM. Improved method for the determination of blood glutathione. J Lab Clin Med 1963; 61: 882–888.
Dubler RE, Anderson BM. Simultaneous inactivation of the catalytic activities of yeast glutathione reductase by N-alkyl meleinindes. Biochemica Biophysica Acta 1981; 659: 70–78.
Habig WH, Pabst MJ, Jocoby WB. The first enzyme step in mercaptouric acid formation. J Biol Chem 1974; 249: 7130–7139.
Necheles TF, Boles TA, Allen DM. Glutathione peroxidase assay method. J Pedia 1968; 72: 319–323.
Bergmeyer HV, Gawehn K, Grassik M, editors. Method of enzymatic analysis. In: Bergmeyer HV, Chemie V, Weinhein S (Eds.). New York: Academic Press, 1994: 348pp.
Misra HP, Fridovich I. The role of superoxide anion in the autooxidation of epinephrine and a simple assay for superoxide dismutase. J Biol Chem 1972; 247: 3170–3185.
Ohkawa H, Onishi N, Yagi K. Assay of lipid peroxidation in animal tissue by thiobarbituric acid reaction. Analytical Biochem 1979; 95: 351–354.
Green LC, Wagner DA, Glogowski J, Skipper PL, Wishnok JS, Tannenbaum SR. Analysis of nitrate, nitrite, and [15N] nitrate in biological fluids. Analytical Biochem 1982; 126(1): 131–138.
Johnston RB Jr, Keele BB Jr, Misra HP, Lehmeyer JE, Webb LS, Baehner RL, Rajagopalan KV. The role of superoxide anion generation in phagocytic bactericidal activity. Studies with normal and chronic granulomatous disease leukocytes. J Clin Invest 1975; 55(6): 1357–1372.
Beckman JS, Ischiropoulos H, Zhu L, van der Woerd M, Smith C, Chen J, et al. Kinetics of superoxide dismutase-and iron-catalyzed nitration of phenolics by peroxynitrite. Arch Biochem Biophys 1992; 298(2): 438–445.
Lowry OH, Rosebrough NJ, Farr AL, Randall RJ. Protein measurement with folin-phenol reagent. J Biol Chem 1951; 193: 256–261.
Chauhan S. Pre-clinical trials of certain herbal medicines in the therapy of type-II diabetes mellitus. India: Ph.D. Thesis, Nagpur University, 2002: 105pp.
Nagda KK, Deshmukh B. Hemagglutination pattern of galactose specific lectin from Pedilanthus tithymaloides in diabetes mellitus. Ind J Exp Biol 1998; 36: 426–428.
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Chauhan, S., Nath, N. & Tule, V. Antidiabetic and antioxidant effects of Picrorhiza kurrooa rhizome extracts in diabetic rats. Indian J Clin Biochem 23, 238–242 (2008). https://doi.org/10.1007/s12291-008-0053-z
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DOI: https://doi.org/10.1007/s12291-008-0053-z