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Lysobacter ciconiae sp. nov., and Lysobacter avium sp. nov., isolated from the faeces of an Oriental stork

  • Microbial Systematics and Evolutionary Microbiology
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Abstract

Two Gram-stain-negative, mesophilic, strictly aerobic, nonspore forming, and yellow-pigmented strains with rod-shaped cells, designated H21R20T and H23M41T, were isolated from the faeces of an Oriental stork (Ciconia boyciana). Based on 16S rRNA gene sequences, both strains showed the highest similarity (98.3–98.4%) to the type strain of Lysobacter concretionis. Phylogenetic analysis based on the 16S rRNA genes and 92 bacterial core genes showed that strains H21R20T and H23M41T were robustly clustered with L. concretionis Ko07T. Whole genome sequencing revealed that the genomes of both strains were approximately 2.9 Mb in size. The DNA G + C contents of the H21R20T and H23M41T strains were 67.3 and 66.6%, respectively. The two strains showed 80.1–81.7% average nucleotide identity with L. concretionis Ko07T. Strain H21R20T grew optimally at 30°C and pH 8.0 and in the presence of 0.5–3% (wt/vol) NaCl, while strain H23M41T grew optimally at 30°C and pH 7.0–8.0 and in the presence of 0–3% (wt/vol) NaCl. Both strains possessed iso-C15:0, iso-C16:0 and summed feature 9 (iso-C17:1ω9c and/or C16:010-methyl) as the major cellular fatty acids, ubiquinone Q-8 as a predominant quinone, and diphosphatidylglycerol, phosphatidylglycerol, and phosphatidylethanolamine as the major polar lipids. A multifaceted investigation demonstrated that strains H21R20T and H23M41T represent novel species of the genus Lysobacter, for which we propose the names Lysobacter ciconiae sp. nov. and Lysobacter avium sp. nov. for strains H21R20T (= KCTC 82316T = JCM 34832T) and H23M41T (= KCTC 62676T = JCM 33223T), respectively.

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Acknowledgements

This work was supported by grants from the Mid-Career Researcher Program (NRF-2020R1A2C3012797) through the National Research Foundation of Korea (NRF) and the National Institute of Biological Resources (NIBR201801106) funded by the Ministry of Environment of Korea (MOE). This work was also supported by the NRF grant funded by the Korean government (MSIT) (No. NRF-2018R1A5A1025077).

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  1. Department of Biology and Department of Biomedical and Pharmaceutical Sciences, Kyung Hee University, Seoul, 02447, Republic of Korea

    So-Yeon Lee, Dong-Wook Hyun & Jin-Woo Bae

  2. Department of Life and Nanopharmaceutical Sciences and Department of Biology, Kyung Hee University, Seoul, 02447, Republic of Korea

    Pil Soo Kim, Hojun Sung & Jin-Woo Bae

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Ethical Statement All sampling conducted in this study was approved by the Institutional Animal Care and Use Committee of Kyung Hee University (Permit number: KHUASP(SE)-18-048) and complied with the guidelines of the Committee.

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Lee, SY., Kim, P.S., Sung, H. et al. Lysobacter ciconiae sp. nov., and Lysobacter avium sp. nov., isolated from the faeces of an Oriental stork. J Microbiol. 60, 469–477 (2022). https://doi.org/10.1007/s12275-022-1647-5

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