Skip to main content
SpringerLink
Log in

Changes in the microbial community of Litopenaeus vannamei larvae and rearing water during different growth stages after disinfection treatment of hatchery water

  • Microbial Ecology and Environmental Microbiology
  • Published:
Journal of Microbiology Aims and scope Submit manuscript

Abstract

Microbial communities greatly affect rearing water quality and the larvae health during shrimp hatchery periods. In this study, we investigated the microbial communities of rearing water and larvae of Litopenaeus vannamei after treating hatchery water with different kinds of chemical disinfectants: no disinfectants (Con), chlorine dioxide (ClO2), formaldehyde solution (HCHO), bleach powder (CaClO), and iodine (I2). The water and larval samples were collected from nauplius 6 (N6), zoea 1 (Z1), mysis 1 (M1), and postlarvae 1 (P1) shrimp growth periods. 16S rDNA high-throughput sequencing revealed that the bacterial composition of the rearing water was more complex than that of the larvae, and the bacterial community of the rearing water and the larvae fluctuated significantly at the P1 and Z1 periods, respectively. Disinfectants altered the bacterial diversity and composition of the rearing water and larvae. Specifically, in the rearing water of the P1 period, Proteobacteria abundance was increased in the HCHO group; while Bacteroidetes abundance was decreased in the ClO2, HCHO, and I2 groups but increased in the CaClO group. In the larvae of the Z1 period, Firmicutes (especially Bacillus class) abundance was increased in the CaClO group, but decreased in the ClO2, HCHO, and I2 groups. Network analyses revealed that the genera Donghicola, Roseibacterium, Candidatus-Cquiluna, and Nautella were enriched in the rearing water, while Halomonas, Vibrio, and Flavirhabdus had high abundance in the larvae. The survival of shrimp was influenced by disinfectants that were inconsistent with the bacterial community changes. These results will be helpful for using microbial characteristics to facilitate healthy shrimp nursery.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Similar content being viewed by others

References

  • Ali, H., Rahman, M.M., Rico, A., Jaman, A., Basak, S.K., Islam, M.M., Khan, N., Keus, H.J., and Mohan, C.V. 2018. An assessment of health management practices and occupational health hazards in tiger shrimp (Penaeus monodon) and freshwater prawn (Macrobrachium rosenbergii) aquaculture in Bangladesh. Vet. Anim. Sci. 5, 10–19.

    Article  Google Scholar 

  • APHA, American Public Health Association. 1998. Standard Methods for the Examination of the Water and Wastewater. 20th edn, pp. 3–37. American Public Health Association, American Water Works Association and Water Environmental Federation, Washington DC, USA.

    Google Scholar 

  • Cornejo-Granados, F., Gallardo-Becerra, L., Leonardo-Reza, M., Ochoa-Romo, J.P., and Ochoa-Leyva, A. 2018. A meta-analysis reveals the environmental and host factors shaping the structure and function of the shrimp microbiota. PeerJ 6, e5382.

    Article  CAS  Google Scholar 

  • Cornejo-Granados, F., Lopez-Zavala, A.A., Gallardo-Becerra, L., Mendoza-Vargas, A., Sánchez, F., Vichido, R., Brieba, L., Viana, M.T., Sotelo-Mundo, R.R., and Ochoa-Leyva, A. 2017. Microbiome of Pacific Whiteleg shrimp reveals differential bacterial community composition between wild, aquacultured and AHPND/EMS outbreak conditions. Sci. Rep. 7, 11783.

    Article  CAS  Google Scholar 

  • Cottrell, M.T. and Kirchman, D.L. 2000. Natural assemblages of marine Proteobacteria and members of the Cytophaga-Flavobacter cluster consuming low- and high molecular-weight dissolved organic matter. Appl. Environ. Microbiol. 66, 1692–1697.

    Article  CAS  Google Scholar 

  • Duan, Y., Wang, Y., Dong, H., Ding, X., Liu, Q., Li, H., Zhang, J., and Xiong, D. 2018. Changes in the intestine microbial, digestive, and immune-related genes of Litopenaeus vannamei in response to dietary probiotic Clostridium butyricum supplementation. Front. Microbiol. 9, 2191.

    Article  Google Scholar 

  • Duan, Y., Zhang, J., Dong, H., Wang, Y., Liu, Q., and Li, H. 2015. Oxidative stress response of the black tiger shrimp Penaeus monodon to Vibrio parahaemolyticus challenge. Fish Shellfish Immun. 46, 354–365.

    Article  CAS  Google Scholar 

  • Duan, Y., Zhang, Y., Dong, H., Wang, Y., and Zhang, J. 2017. Effects of dietary poly-β-hydroxybutyrate (PHB) on microbiota composition and the mTOR signaling pathway in the intestines of Litopenaeus vannamei. J. Microbiol. 55, 946–954.

    Article  CAS  Google Scholar 

  • Edgar, R.C. 2013. UPARSE: highly accurate OTU sequences from microbial amplicon reads. Nat. Methods 10, 996–998.

    Article  CAS  Google Scholar 

  • Edgar, R.C., Haas, B.J., Clemente, J.C., Quince, C., and Knight, R. 2011. UCHIME improves sensitivity and speed of chimera detection. Bioinformatics 27, 2194–2200.

    Article  CAS  Google Scholar 

  • Gao, M., Du, D., Bo, Z., and Sui, L. 2019. Poly-β-hydroxybutyrate (PHB)-accumulating Halomonas improves the survival, growth, robustness and modifies the gut microbial composition of Litopenaeus vannamei postlarvae. Aquaculture 500, 607–612.

    Article  CAS  Google Scholar 

  • Gao, S., Pan, L., Huang, F., Song, M., Tian, C., and Zhang, M. 2019. Metagenomic insights into the structure and function of intestinal microbiota of the farmed Pacific white shrimp (Litopenaeus vannamei). Aquaculture 499, 109–118.

    Article  CAS  Google Scholar 

  • Gräslund, S. and Bengtsson, B.E. 2001. Chemicals and biological products used in south-east Asian shrimp farming, and their potential impact on the environment — a review. Sci. Total Environ. 280, 93–131.

    Article  Google Scholar 

  • Hou, D., Huang, Z., Zeng, S., Liu, J., Wei, D., Deng, X., Weng, S., He, Z., and He, J. 2017. Environmental factors shape water microbial community structure and function in shrimp cultural enclosure ecosystems. Front. Microbiol. 8, 2359.

    Article  Google Scholar 

  • Hsiao, E.Y., McBride, S.W., Hsien, S., Sharon, G., Hyde, E.R., McCue, T., Codelli, J.A., Chow, J., Reisman, S.E., Petrosino, J.F., et al. 2013. Microbiota modulate behavioral and physiological abnormalities associated with neurodevelopmental disorders. Cell 155, 1451–1463.

    Article  CAS  Google Scholar 

  • Joulak, I., Finore, I., Nicolaus, B., Leone, L., Moriello, A.S., Attia, H., Poli, A., and Azabou, S. 2019. Evaluation of the production of exopolysaccharides by newly isolated Halomonas strains from Tunisian hypersaline environments. Int. J. Biol. Macromol. 138, 658–666.

    Article  CAS  Google Scholar 

  • Koeth, R.A., Wang, Z., Levison, B.S., Buffa, J.A., Org, E., Sheehy, B.T., Britt, E.B., Fu, X., Wu, Y., Li, L., et al. 2013. Intestinal microbiota metabolism of L-carnitine, a nutrient in red meat, promotes atherosclerosis. Nat. Med. 19, 576–585.

    Article  CAS  Google Scholar 

  • Li, J., Tan, B., and Mai, K. 2009. Dietary probiotic Bacillus OJ and isomaltooligosaccharides influence the intestine microbial populations, immune responses and resistance to white spot syndrome virus in shrimp (Litopenaeus vannamei). Aquaculture 291, 35–40.

    Article  CAS  Google Scholar 

  • Lightner, D.V. and Redman, R.M. 1998. Shrimp diseases and current diagnostic methods. Aquaculture 164, 201–220.

    Article  Google Scholar 

  • Rios-Del Toro, E.E., López-Lozano, N.E., and Cervantes, F.J. 2017. Up-flow anaerobic sediment trapped (UAST) reactor as a new configuration for the enrichment of anammox bacteria from marine sediments. Bioresour. Technol. 238, 528–533.

    Article  CAS  Google Scholar 

  • Van Haute, S., Tryland, I., Escudero, C., Vanneste, M., and Sampers, I. 2017. Chlorine dioxide as water disinfectant during fresh-cut iceberg lettuce washing: disinfectant demand, disinfection efficiency, and chlorite formation. LWT 75, 301–304.

    Article  CAS  Google Scholar 

  • Vandenberghe, J., Verdonck, L., Robles-Arozarena, R., Rivera, G., Bolland, A., Balladares, M., Gomez-Gil, B., Calderon, J., Sorgeloos, P., and Swings, J. 1999. Vibrios associated with Litopenaeus vannamei larvae, postlarvae, broodstock, and hatchery probionts. Appl. Environ. Microbiol. 65, 2592–2597.

    Article  CAS  Google Scholar 

  • Wang, J., Huang, Y., Xu, K., Zhang, X., Sun, H., Fan, L., and Yan, M. 2019. White spot syndrome virus (WSSV) infection impacts intestinal microbiota composition and function in Litopenaeus vannamei. Fish Shellfish Immun. 84, 130–137.

    Article  Google Scholar 

  • Williams, T.J., Wilkins, D., Long, E., Evans, F., DeMaere, M.Z., Raftery, M.J., and Cavicchioli, R. 2013. The role of planktonic Flavobacteria in processing algal organic matter in coastal East Antarctica revealed using metagenomics and metaproteomics. Environ. Microbiol. 15, 1302–1317.

    Article  CAS  Google Scholar 

  • Xiong, J., Wang, K., Wu, J., Qiuqian, L., Yang, K., Qian, Y., and Zhang, D. 2015. Changes in intestinal bacterial communities are closely associated with shrimp disease severity. Appl. Microbiol. Biotechnol. 99, 6911–6919.

    Article  CAS  Google Scholar 

  • Zecher, K., Suleiman, M., Wibberg, D., Winkler, A., Philipp, B., and Kalinowski, J. 2017. Draft genome sequence of Donghicola sp. strain KarMa, a model organism for monomethylamine-degrading nonmethylotrophic bacteria. Genome Announc. 5, e01623–16.

    Article  Google Scholar 

  • Zhang, M., Pan, L., Huang, F., Gao, S., Su, C., Zhang, M., and He, Z. 2019. Metagenomic analysis of composition, function and cycling processes of microbial community in water, sediment and effluent of Litopenaeus vannamei farming environments under different culture modes. Aquaculture 506, 280–293.

    Article  Google Scholar 

  • Zheng, Y., Yu, M., Liu, J., Qiao, Y., Wang, L., Li, Z., Zhang, X.H., and Yu, M. 2017. Bacterial community associated with healthy and diseased Pacific white shrimp (Litopenaeus vannamei) larvae and rearing water across different growth stages. Front. Microbiol. 8, 1362.

    Article  Google Scholar 

  • Zheng, Y., Yu, M., Liu, Y., Su, Y., Xu, T., Yu, M.C., and Zhang, X.H. 2016. Comparison of cultivable bacterial communities associated with Pacific white shrimp (Litopenaeus vannamei) larvae at different health statuses and growth stages. Aquaculture 451, 163–169.

    Article  Google Scholar 

  • Zothanpuia, Passari, A.K., Leo, V.V., Chandra, P., Kumar, B., Nayak, C., Hashem, A., Abd Allah, E.F., Alqarawi, A.A., and Singh, B.P. 2018. Bioprospection of actinobacteria derived from freshwater sediments for their potential to produce antimicrobial compounds. Microb. Cell Fact. 17, 68.

    Article  CAS  Google Scholar 

Download references

Acknowledgments

This study was supported by the earmarked fund for Central Public-interest Scientific Institution Basal Research Fund, CAFS (2020TD30); Shenzhen Science and Technology Planning Project (JCYJ20170412110605075, JCYJ2018050417-0233070); National Natural Science Foundation of China (31902343); Guangzhou Rural Science and Technology Commissioner Project (GZKTP201813). The authors are grateful to the laboratory staff for technical assistance.

Author information

Authors and Affiliations

  1. Shenzhen Base of South China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Shenzhen, 518121, P. R. China

    Yafei Duan, Yapeng Tang, Jianhua Huang, Jiasong Zhang & Heizhao Lin

  2. Key Laboratory of South China Sea Fishery Resources Exploitation & Utilization, Ministry of Agriculture and Rural Affairs, Guangdong Provincial Key Laboratory of Fishery Ecology and Environment, South China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Guangzhou, 510300, P. R. China

    Yafei Duan, Yapeng Tang, Jianhua Huang, Jiasong Zhang, Heizhao Lin, Shigui Jiang & Ruixuan Wang

  3. Hainan Academy of Ocean and Fisheries Sciences, Haikou, 571126, P. R. China

    Guofu Wang

Authors
  1. Yafei Duan
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Yapeng Tang
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Jianhua Huang
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Jiasong Zhang
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Heizhao Lin
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Shigui Jiang
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. Ruixuan Wang
    View author publications

    You can also search for this author in PubMed Google Scholar

  8. Guofu Wang
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding authors

Correspondence to Jianhua Huang, Jiasong Zhang or Guofu Wang.

Additional information

Conflict of Interest

The authors declare that they have no competing interests.

Supplemental material for this article may be found at http://www.springerlink.com/content/120956.

Electronic Supplementary Material

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Duan, Y., Tang, Y., Huang, J. et al. Changes in the microbial community of Litopenaeus vannamei larvae and rearing water during different growth stages after disinfection treatment of hatchery water. J Microbiol. 58, 741–749 (2020). https://doi.org/10.1007/s12275-020-0053-0

Download citation

  • Received:

  • Revised:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s12275-020-0053-0

Keywords

Search

Navigation