Abstract
A novel actinobacterium, strain SK68, was isolated from the rhizosphere of peanut plant and its salinity stress alleviation ability was studied using tomato (Solanum lycopersicum cv. Micro-Tom) plants. Based on 16S rDNA based phylogenetic analysis, strain SK68 has been identified as a Streptomyces sp. Strain SK68 had branched substrate mycelium bearing smooth surfaced spores and the spore colour is brownish grey on ISP4 medium. It exhibited enzyme activities such as xylanase, cellulase, amylase, and pectinase and degraded hypoxanthine, casein, and L-tyrosine. The strain SK68 differed in its banding pattern in BOX-PCR and RAPD fingerprinting compared to the closely matching type strains Streptomyces erythrochromogenes NBRC 3304T (AB184746), S. flavotricini NBRC 12770T (AB184132), S. racemochromogenes NBRC 12906T (AB184235), and S. polychromogenes NBRC 13072T (NR041109). Strain SK68 was evaluated for its salinity stress-alleviating activity in tomato plants with 180 mmol/L NaCl under gnotobiotic condition. A significant increase in plant biomass was observed in strain SK68-inoculated tomato plants under salt stress compared to control and salt-stressed non-inoculated plants.
Similar content being viewed by others
References
Al-Tai, A., Kim, B., Kim, S.B., Manfio, G.P., and Goodfellow, M. 1999. Streptomyces malaysiensis sp. nov., a new streptomycete species with rugose, ornamented spores. Int. J. Syst. Evol. Microbiol. 49, 1395–1402.
Alvarez, A., Yanez, M., Benimeli, C., and Amoroso, M. 2012. Maize plants (Zea mays) root exudates enhance lindane removal by native Streptomyces strains. Int. Biodeterior. Biodegrad. 66, 14–18.
Arkhipova, T., Veselov, S., Melentiev, A., Martynenko, E., and Kudoyarova, G. 2005. Ability of bacterium Bacillus subtilis to produce cytokinins and to influence the growth and endogenous hormone content of lettuce plants. Plant Soil 272, 201–209.
Athalye, M., Noble, W.C., and Minnikin, D.E. 1985. Analysis of cellular fatty acids by gas chromatography as a tool in the identification of medically important coryneform bacteria. J. Appl. Bacteriol. 58, 507–512.
Berdy, J. 2005. Bioactive microbial metabolites. J. Antibiot. 58, 1.
Chung, E.J., Hossain, M.T., Khan, A., Kim, K.H., Jeon, C.O., and Chung, Y.R. 2015. Bacillus oryzicola sp. nov., an endophytic bacterium isolated from the roots of rice with antimicrobial, plant growth promoting, and systemic resistance inducing activities in rice. Plant Pathol. J. 31, 152.
Colla, G., Rouphael, Y., Di Mattia, E., El-Nakhel, C., and Cardarelli, M. 2015. Co–inoculation of Glomus intraradices and Trichoderma atroviride acts as a biostimulant to promote growth, yield and nutrient uptake of vegetable crops. J. Sci. Food Agric. 95, 1706–1715.
Deinlein, U., Stephan, A.B., Horie, T., Luo, W., Xu, G., and Schroeder, J.I. 2014. Plant salt-tolerance mechanisms. Trends Plant Sci. 19, 371–379.
Gherbawy, Y., Elhariry, H., Altalhi, A., El-Deeb, B., and Khiralla, G. 2012. Molecular screening of Streptomyces isolates for antifungal activity and family 19 chitinase enzymes. J. Microbiol. 50, 459–468.
Gonzalez, J. and Saiz–Jimenez, C. 2002. A fluorimetric method for the estimation of G + C mol% content in microorganisms by thermal denaturation temperature. Environ. Microbiol. 4, 770–773.
Gonzalez, J.M. and Saiz-Jimenez, C. 2005. A simple fluorimetric method for the estimation of DNA-DNA relatedness between closely related microorganisms by thermal denaturation temperatures. Extremophiles 9, 75–79.
Gopalakrishnan, S., Srinivas, V., Alekhya, G., Prakash, B., Kudapa, H., Rathore, A., and Varshney, R.K. 2015. The extent of grain yield and plant growth enhancement by plant growth-promoting broad-spectrum Streptomyces sp. in chickpea. SpringerPlus 4, 31.
Han, J.H., Hwang, I.C., Cho, S.H., Jang, C., Kim, N.G., Yu, S.H., Yu, Y.M., and Kim, S.B. 2008. Description of Streptomyces neopeptinius sp. nov., an actinobacterium with broad spectrum antifungal activities. J. Microbiol. 46, 295.
Hasegawa, T., Takizawa, M., and Tanida, S. 1983. A rapid analysis for chemical grouping of aerobic actinomycetes. J. Gen. Appl. Microbiol. 29, 319–322.
Hsu, S. and Lockwood, J. 1975. Powdered chitin agar as a selective medium for enumeration of actinomycetes in water and soil. Appl. Microbiol. 29, 422–426.
Jog, R., Pandya, M., Nareshkumar, G., and Rajkumar, S. 2014. Mechanism of phosphate solubilization and antifungal activity of Streptomyces spp. isolated from wheat roots and rhizosphere and their application in improving plant growth. Microbiology 160, 778–788.
Kampfer, P. and Kroppenstedt, R.M. 1996. Numerical analysis of fatty acid patterns of coryneform bacteria and related taxa. Can. J. Microbiol. 42, 989–1005.
Kasana, R.C., Salwan, R., Dhar, H., Dutt, S., and Gulati, A. 2008. A rapid and easy method for the detection of microbial cellulases on agar plates using Gram’s iodine. Curr. Microbiol. 57, 503–507.
Ley, J.D., Cattoir, H., and Reynaerts, A. 1970. The quantitative measurement of DNA hybridization from renaturation rates. FEBS J. 12, 133–142.
Martin, P., Dary, A., André, A., and Decaris, B. 2000. Identification and typing of Streptomyces strains: evaluation of interspecific, intraspecific and intraclonal differencesby RAPD fingerprinting. Res. Microbiol. 151, 853–864.
Mickelbart, M.V., Hasegawa, P.M., and Bailey-Serres, J. 2015. Genetic mechanisms of abiotic stress tolerance that translate to crop yield stability. Nat. Rev. Gen. 16, 237.
Palaniyandi, S., Damodharan, K., Yang, S., and Suh, J. 2014. Streptomyces sp. strain PGPA39 alleviates salt stress and promotes growth of ‘Micro Tom’ tomato plants. J. Appl. Microbiol. 117, 766–773.
Palaniyandi, S.A., Yang, S.H., Zhang, L., and Suh, J.W. 2013. Effects of actinobacteria on plant disease suppression and growth promotion. Appl. Microbiol. Biotechnol. 97, 9621–9636.
Roberts, M.A. and Crawford, D.L. 2000. Use of randomly amplified polymorphic DNA as a means of developing genus-and strainspecific Streptomyces DNA probes. Appl. Environ. Microbiol. 66, 2555–2564.
Saitou, N. and Nei, M. 1987. The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol. Biol. Evol. 4, 406–425.
Shirling, E.T. and Gottlieb, D. 1966. Methods for characterization of Streptomyces species. Int. J. Syst. Evol. Microbiol. 16, 313–340.
Shrivastava, P. and Kumar, R. 2015. Soil salinity: a serious environmental issue and plant growth promoting bacteria as one of the tools for its alleviation. Saudi J. Biol. Sci. 22, 123–131.
Staneck, J.L. and Roberts, G.D. 1974. Simplified approach to identification of aerobic actinomycetes by thin-layer chromatography. Appl. Microbiol. 28, 226–231.
Tamura, K., Stecher, G., Peterson, D., Filipski, A., and Kumar, S. 2013. MEGA6: molecular evolutionary genetics analysis version 6.0. Mol. Biol. Evol. 30, 2725–2729.
Thompson, J.D., Gibson, T., and Higgins, D.G. 2002. Multiple sequence alignment using ClustalW and ClustalX. Curr. Protoc. Bioinformatics 2.3.1–2.3.22.
Vijayabharathi, R., Bruheim, P., Andreassen, T., Raja, D.S., Devi, P.B., Sathyabama, S., and Priyadarisini, V.B. 2011. Assessment of resistomycin, as an anticancer compound isolated and characterized from Streptomyces aurantiacus AAA5. J. Microbiol. 49, 920–926.
Waksman, S.A. and Henrici, A.T. 1943. The nomenclature and classification of the actinomycetes. J. Bacteriol. 46, 337.
Williams, S.T. 1989. Genus Streptomyces waksman and henrici1943. Bergey’s manual of systematic bacteriology 4, 2452–2492.
Yang, J., Kloepper, J.W., and Ryu, C.M. 2009. Rhizosphere bacteria help plants tolerate abiotic stress. Trends Plant Sci. 14, 1–4.
Zhu, J.K. 2001. Plant salt tolerance. Trends Plant Sci. 6, 66–71.
Author information
Authors and Affiliations
Corresponding authors
Additional information
Supplemental material for this article may be found at https://doi.org/www.springerlink.com/content/120956.
Electronic supplementary material
Rights and permissions
About this article
Cite this article
Damodharan, K., Palaniyandi, S.A., Le, B. et al. Streptomyces sp. strain SK68, isolated from peanut rhizosphere, promotes growth and alleviates salt stress in tomato (Solanum lycopersicum cv. Micro-Tom). J Microbiol. 56, 753–759 (2018). https://doi.org/10.1007/s12275-018-8120-5
Received:
Revised:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s12275-018-8120-5