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Characterization of recombinant β-glucosidase from Arthrobacter chlorophenolicus and biotransformation of ginsenosides Rb1, Rb2, Rc, and Rd

  • Microbial Physiology and Biochemistry
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Abstract

The focus of this study was the cloning, expression, and characterization of recombinant ginsenoside hydrolyzing β-glucosidase from Arthrobacter chlorophenolicus with an ultimate objective to more efficiently bio-transform ginsenosides. The gene bglAch, consisting of 1,260 bp (419 amino acid residues) was cloned and the recombinant enzyme, overexpressed in Escherichia coli BL21 (DE3), was characterized. The GST-fused BglAch was purified using GST·Bind agarose resin and characterized. Under optimal conditions (pH 6.0 and 37°C) BglAch hydrolyzed the outer glucose and arabinopyranose moieties of ginsenosides Rb1 and Rb2 at the C20 position of the aglycone into ginsenoside Rd. This was followed by hydrolysis into F2 of the outer glucose moiety of ginsenoside Rd at the C3 position of the aglycone. Additionally, BglAch more slowly transformed Rc to F2 via C-Mc1 (compared to hydrolysis of Rb1 or Rb2). These results indicate that the recombinant BglAch could be useful for the production of ginsenoside F2 for use in the pharmaceutical and cosmetic industries.

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Authors and Affiliations

  1. Department of Biological Sciences, Korea Advanced Institute of Science and Technology, Daejeon, 305-701, Republic of Korea

    Myung Keun Park, Chang-Hao Cui, Suk-Chae Jung & Sun-Chang Kim

  2. Intelligent Synthetic Biology Center, Daejeon, 305-701, Republic of Korea

    Sung Chul Park, Suk-Chae Jung & Sun-Chang Kim

  3. KAIST Institute for Biocentury, Korea Advanced Institute of Science and Technology, Daejeon, 305-701, Republic of Korea

    Seul-Ki Park, Jin-Kwang Kim & Sun-Chang Kim

  4. Youngdong University, 310, 370-701, Chungbuk, Republic of Korea

    Mi-Sun Jung

  5. Department of Biotechnology, Hankyoung National Univeristy, Kyonggi-do, 456-749, Republic of Korea

    Wan-Taek Im

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  1. Myung Keun Park
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Park, M.K., Cui, CH., Park, S.C. et al. Characterization of recombinant β-glucosidase from Arthrobacter chlorophenolicus and biotransformation of ginsenosides Rb1, Rb2, Rc, and Rd. J Microbiol. 52, 399–406 (2014). https://doi.org/10.1007/s12275-014-3601-7

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  • DOI: https://doi.org/10.1007/s12275-014-3601-7

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