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Purification and characterization of a novel laccase from the edible mushroom Hericium coralloides

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Abstract

A novel laccase from the edible mushroom Hericium coralloides was purified by ion exchange chromatography on diethylaminoethyl (DEAE) cellulose, carboxymethyl (CM) cellulose, and Q-Sepharose columns followed by fast protein liquid chromatography gel filtration on a Superdex 75 column. Analysis by gel filtration and SDS-PAGE indicated that the protein is a monomer in solution with a molecular mass of 65 kDa. Its N-terminal amino acid sequence was AVGDDTPQLY, which exhibits partial sequence homology to previously isolated laccases. Optimum activity was observed at pH 2.2 and at 40°C. The enzyme showed activity toward a variety of substrates, the most sensitive of which was 2,2′-azinobis [3-ethylbenzothiazolone-6-sulfonic acid] diammonium salt (ABTS). The degradation activity toward substrates was ABTS > N,N-dimethyl-1,4-phenylenediamine > catechol > 2-methylcatechol > pyrogallol. The laccase did not exert any antiproliferative activity against Hep G2 or MCF 7 tumor cell lines at a concentration of 60 μM, unlike some previously reported mushroom proteins, but showed significant activity toward human immunodeficiency virus-1 (HIV-1) reverse transcriptase with an IC50 of 0.06 μM.

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Authors and Affiliations

  1. Institute of Agricultural Resources and Regional Planning, Chinese Academy of Agricultural Sciences, Beijing, 100081, P. R. China

    Ya-Jie Zou, Chen-Yang Huang & Jin-Xia Zhang

  2. State Key Laboratory for Agrobiotechnology, College of Biological Sciences, China Agricultural University, Beijing, 100193, P. R. China

    He-Xiang Wang

  3. Department of Biochemistry, Faculty of Medicine, The Chinese University of Hong Kong, Shatin, New Territories, Hong Kong, P. R. China

    Tzi-Bun Ng

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  1. Ya-Jie Zou
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  2. He-Xiang Wang
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  3. Tzi-Bun Ng
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  4. Chen-Yang Huang
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  5. Jin-Xia Zhang
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Correspondence to Jin-Xia Zhang.

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Zou, YJ., Wang, HX., Ng, TB. et al. Purification and characterization of a novel laccase from the edible mushroom Hericium coralloides . J Microbiol. 50, 72–78 (2012). https://doi.org/10.1007/s12275-012-1372-6

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  • DOI: https://doi.org/10.1007/s12275-012-1372-6

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