Abstract
A Gram-negative, non-motile, rod shaped, and orange-pigmented chemoheterotrophic bacterium, strain MS-31T was isolated from the marine sponge Hymeniacidon flavia, collected from near Jeju Island, Korea. The Strain MS-31T was subjected to a polyphasic taxonomic study. The phylogenetic analysis based on the 16S rRNA gene sequences revealed that the novel isolate could be affiliated within the genus Sphingomonas. The strain MS-31T showed 95.6% of 16S rRNA gene sequence similarity with the most closely related species Sphingomonas koreensis JSS26T. The DNA G+C content of the strain MS-31T was 69.4 mol%. The major isoprenoid quinone was ubiqunone 10 and predominant cellular fatty acids were summed feature 7 (comprising C18:1 ω7c, C18:1 Ω9t and/or C18:1 ωl2t, 39.7%), C16:0 (16.3%), C14:0 2OH (15.9%) and summed feature 3 (comprising C16:1 ω7c and/or C15:0 iso 2OH, 11.7%). The polar lipids were sphingoglycolipid, phosphatidyletha-nolamine, phosphatidylglycerol, diphosphatidylglycerol and unidentified glycolipid. Based on the evidence from the polyphasic taxonomic study, the strain should be classified as a new species of the genus Sphingomonas. As a result, the name Sphingomonas jejuensis sp. nov. (type strain MS-31T =KCTC 23321T =NBRC 107775T) is proposed.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Denner, E.B., S. Paukner, P. Kämpfer, E.R. Moore, W.R. Abraham, H.J. Busse, G. Wanner, and W. Lubitz. 2001. Sphingomonas pituitosa sp. nov., an exopolysaccharide-producing bacterium that secretes an unusual type of sphingan. Int. J. Syst. Evol. Microbiol. 51, 827–884.
Dittmer, J.C. and L.A. Lester. 1964. A simple, specific spray for the detection of phospholipids on thin-layer chromatograms. J. Lipid Res. 15, 126–127.
Felsenstein, J. 1985. Confidence limits on phylogenies: an approach using the bootstrap. Evolution 39, 783–791.
Kimura, M. 1983. The Neutral Theory of Molecular Evolution. Cambridge: Cambridge University Press.
Komagata, K. and K. Suzuki. 1987. Lipid and cell-wall analysis in bacterial systematics. Methods Microbiol. 19, 161–207.
Lee, J.S., Y.K. Shin, J.H. Yoon, M. Takeuchi, Y.R. Pyun, and Y.H. Park. 2001. Sphingomonas aquatilis sp. nov., Sphingomonas koreensis sp. nov., and Sphingomonas taejonensis sp. nov., yellow-pigmented bacteria isolated from natural mineral water. Int. J. Syst. Evol. Microbiol. 51, 1491–1498.
Nigam, A., S. Jit, and R. Lal. 2010. Sphingomonas histidinilytica sp. nov., isolated from a hexachlorocyclohexane dump site. Int. J. Syst. Evol. Microbiol. 60, 1038–1043.
Marmur, J. 1961. A procedure for the isolation of deoxyribonucleic acid from microorganisms. J. Mol. Biol. 3, 208–218.
Mesbah, M., U. Premachandran, and W.B. Whitman. 1989. Precise measurement of the G+C content of deoxyribonucleic acid by high-performance liquid chromatography. Int. J. Syst. Bacteriol. 39, 159–167.
Minnikin, D.E., A.G. O’Donnell, M. Goodfellow, G. Alderson, M. Athalye, A. Schaal, and J.H. Parlett. 1984. An integrated procedure for the extraction of bacterial isoprenoid quinones and polar lipids. J. Microbiol. Methods 2, 233–241.
Murray, R.G.E., R.N. Doetsch, and F. Robinow. 1994. Determinative and cytological light microscopy, Methods for General and Molecular Bacteriology, pp. 21–41. In P. Gerhardt, R.G.E. Murray, W.A. Wood, and N.R. Krieg (eds.), American Society for Microbiology, Washington, DC, USA.
Romanenko, L.A., M. Uchino, G.M. Frolova, N. Tanaka, N.I. Kalinovskaya, N. Latyshev, and V.V. Mikhailov. 2007. Sphingomonas molluscorum sp. nov., a novel marine isolate with antimicrobial activity. Int. J. Syst. Evol. Microbiol. 57, 358–363.
Saitou, N. and M. Nei. 1987. The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol. Biol. Evol. 4, 406–425.
Sasser, M. 1990. Identification of bacteria by gas chromatography of cellular fatty acids, MIDI Technical Note 101. MIDI Inc. Newark, DE, USA.
Takeuchi, M., T. Sakane, M. Yanagi, K. Yamasato, K. Hamana, and A. Yokota. 1995. Taxonomic study of bacteria isolated from plants: proposal of Sphingomonas rosa sp. nov., Sphingomonas pruni sp. nov., Sphingomonas asaccharolytica sp. nov., and Sphingomonas mali sp. nov. Int. J. Syst. Bacteriol. 45, 334–341.
Tamura, K., J. Dudley, M. Nei, and S. Kumar. 2007 MEGA 4: Molecular Evolutionary Genetics Analysis (MEGA) software version 4.0. Mol. Biol. Evol. 24, 1596–1599.
Thompson, J.D., T.J. Gibson, F. Plewniak, F. Jeanmougin, and D.G. Higgins. 1997. The CLUSTAL_X windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res. 25, 4876–4882.
Weisburg, W.G., S.M. Barns, D.A. Pelletier, and D.J. Lane. 1991. 16S ribosomal DNA amplification for phylogenetic study. J. Bacteriol. 173, 697–703.
Worliczek, H.L., P. Kampfer, R. Rosengarten, R.B.J. Tindall, and H.J. Busse. 2007. Polar lipid and fatty acid profiles-re-vitalizing old approaches as a modern tool for the classification of mycoplasmas? Syst. Appl. Microbiol. 30, 355–370.
Xie, C. and A. Yokota. 2003. Phylogenetic analysis of Lampropedia hyalina based on the 16S rRNA gene sequence. J. Gen. Appl. Microbiol. 49, 345–349.
Yabuuchi, E., I. Yano, H. Oyaizu, Y. Hashimoto, T. Ezaki, and H. Yamamoto. 1990. Proposals of Sphingomonas paucimobilis gen. nov. and comb. nov., Sphingomonas parapaucimobilis sp. nov., Sphingomonas yanoikuyae sp. nov., Sphingomonas adhaesiva sp. nov., Sphingomonas capsulata comb. nov., and two genospecies of the genus Sphingomonas. Microbiol. Immunol. 34, 99–119.
Yoon, J.H., M.H. Lee, S.J. Kang, S.Y. Lee, and T.K. Oh. 2006. Sphingomonas dokdonensis sp. nov., isolated from soil. Int. J. Syst. Evol. Microbiol. 56, 2165–2169.
Yoon, J., M. Yasumoto-Hirose, Y. Matsuo, M. Nozawa, S. Matsuda, H. Kasai, and A. Yokota. 2007. Pelagicoccus mobilis gen. nov., sp. nov., Pelagicoccus albus sp. nov. and Pelagicoccus litoralis sp. nov., three novel members of subdivision 4 within the phylum ‘Verrucomicrobia’, isolated from seawater by in situ cultivation. Int. J. Syst. Evol. Microbiol. 57, 1377–1385.
Zhang, J., X. Liu, and S. Liu. 2010. Sphingomonas changbaiensis sp. nov., isolated from forest soil. Int. J. Syst. Evol. Microbiol. 60, 790–795.
Author information
Authors and Affiliations
Corresponding author
Additional information
Supplemental material for this article may be found at http://www.springer.com/content/120956
Electronic supplementary material
Rights and permissions
About this article
Cite this article
Park, S., Yokota, A., Itoh, T. et al. Sphingomonas jejuensis sp. nov., isolated from marine sponge Hymeniacidon flavia. J Microbiol. 49, 238–242 (2011). https://doi.org/10.1007/s12275-011-0500-z
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s12275-011-0500-z