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Inhibitory effect of the essential oil from Chamaecyparis obtusa on the growth of food-borne pathogens

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Abstract

In this study, the antibacterial activity of essential oil from Chamaecyparis obtusa (Sieb. et Zucc) leaves and twigs was investigated. The test strains were Klebsiella pneumoniae, Listeria monocytogenes, Salmonella typhimurium, Staphylococcus aureus, Escherichia coli, Legionella pneumophila, and Methicilline-resistant Staphylococcus aureus. Antibacterial activity was estimated by measuring bacterial growth inhibition. Histopathological examination was also performed. C. obtusa oil distinctly inhibited the growth of all test strains and exhibited the strongest antibacterial activity against L. monocytogenes. It was chromatographically divided into several fractions. The fractions were further tested against antibacterial activity and their chemical compositions were analyzed. The fraction containing terpinen-4-ol (TA) showed high antibacterial activity toward all strains tested. Tests with authentic samples showed that TA played a major role in the antibacterial activity of C. obtusa oil, and in a mice test, the oil actively minimized inflammation by S. aureus.

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Authors and Affiliations

  1. Division of Green Business Management, Department of Forest Resources Utilization, Korea Forest Research Institute, Seoul, 130-712, Republic of Korea

    Mi-Jin Park, Won-Sil Choi & Ha-Young Kang

  2. Department of Forest Sciences, College of Agriculture and Life Sciences, Seoul National University, Seoul, 151-921, Republic of Korea

    Ki-Seob Gwak & In-Gyu Choi

  3. College of Veterinary Medicine, Chungbuk National University, Chungbuk, 361-763, Republic of Korea

    Geun-Shik Lee & Eui-Bae Jeung

  4. Research Institute for Agriculture and Life Sciences, College of Agriculture and Life Sciences, Seoul National University, Seoul, 151-921, Republic of Korea

    In-Gyu Choi

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  1. Mi-Jin Park
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  2. Won-Sil Choi
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  3. Ha-Young Kang
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  4. Ki-Seob Gwak
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  5. Geun-Shik Lee
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  6. Eui-Bae Jeung
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  7. In-Gyu Choi
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Correspondence to In-Gyu Choi.

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Park, MJ., Choi, WS., Kang, HY. et al. Inhibitory effect of the essential oil from Chamaecyparis obtusa on the growth of food-borne pathogens. J Microbiol. 48, 496–501 (2010). https://doi.org/10.1007/s12275-010-9327-2

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  • DOI: https://doi.org/10.1007/s12275-010-9327-2

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