Abstract
The species concept of Aspergillus fumigatus sensu stricto has recently been defined by polyphasic taxonomy. Based on the new concept of species delimitations, 146 worldwide strains of Aspergillus fumigatus sensu lato were re-identified. Of those 146 strains, 140 (95.8%) could be identified as A. fumigatus sensu stricto, 3 (2.1%) as A. lentulus, and the remaining 3 strains as A. viridinutans complex, Neosartorya udagawae, and N. cf. nishimurae. Of 98 clinical strains, only 1 from dolphin nostril was identified as A. lentulus and not A. fumigatus sensu stricto. Random amplification of polymorphic DNA-polymerase chain reaction (RAPD-PCR) with primers PELF and URP1F produced nearly the same band patterns among 136 strains of A. fumigatus sensu stricto while discriminated the species from its related species. We also discussed about identification of several atypical A. fumigatus strains from clinical environments.
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References
Balajee, S.A., J.L. Gribskov, E. Hanley, D. Nickle, and K.A. Marr. 2005a. Aspergillus lentulus sp. nov., a new sibling species of A. fumigatus. Eukaryot. Cell 4, 625–632.
Balajee, S.A., J.L. Gribskov, M. Brandt, J. Ito, A. Fothergill, and K.A. Marr. 2005b. Mistaken identity: Neosartorya pseudofischeri and its anamorph masquerading as Aspergillus fumigatus. J. Clin. Microbiol. 43, 5996–5999.
Chim, C.S., P.L. Ho, and K.Y. Yuen. 1998. Simultaneous Aspergillus fischeri and herpes simplex pneumonia in a patient with multiple myeloma. Scand. J. Infect. Dis. 30, 190–191.
Coriglione, G., G. Stella, L. Gafa, G. Spata, S. Oliveri, A.A. Padhye, and L. Ajello. 1990. Neosartorya fischeri var. fischeri as a cause of mycotic keratitis. Eur. J. Epidemiol. 6, 382–385.
Debeaupuis, J.P., J. Sarfati, V. Chazalet, and J.P. Latge. 1997. Genetic diversity among clinical and environmental isolates of Aspergillus fumigatus. Infect. Immun. 65, 3080–3085.
Geiser, D.M., J.C. Frisvad, and J.W. Taylor. 1998. Evolutionary relationships in Aspergillus section Fumigati inferred from partial β-tubulin and hydrophobin DNA sequences. Mycologia 90, 831–845.
Gerber, J., J. Chomicki, J.W. Brandsberg, R. Jones, and K.J. Hammerman. 1973. Pulmonary aspergillosis caused by Aspergillus fischeri var. spinosus. Am. J. Clin. Pathol. 60, 861–866.
Gori, S., G. Pellegrini, F. Filipponi, S.D. Capanna, G. Biancofiore, F. Mosca, and A. Lofaro. 1998. Pulmonary aspergillosis caused by Neosartorya fischeri (Aspergillus fischerians) in a liver transplant recipient. J. Mycol. Med. 8, 105–107.
Hong, S.B., H.S. Cho, H.D. Shin, J.C. Frisvad, and R.A. Samson. 2006. Novel Neosartorya species isolated from soil in Korea. Int. J. Syst. Evol. Microbiol. 56, 477–486.
Hong, S.B., S.J. Go, H.D. Shin, J.C. Frisvad, and R.A. Samson. 2005. Polyphasic taxonomy of Aspergillus fumigatus and related species. Mycologia 97, 1316–1329.
Hong, S.B., D.H. Kim, I.C. Park, R.A. Samson, and H.D. Shin. 2010. Isolation and identification of Aspergillus section Fumigati strains from arable soil in Korea. Mycobiology 38, 1–6.
Kang, H.W., D.S. Park, S.J. Go, and M.Y. Eun. 2002. Fingerprinting of diverse genomes using PCR with universal rice primers generated from repetitive sequence of Korean weedy rice. Mol. Cells 13, 281–287.
Katz, M.E., A.M. Dougall, K. Weeks, and B.F. Cheetham. 2005. Multiple genetically distinct groups revealed among clinical isolated identified as atypical Aspergillus fumigatus. J. Clin. Micobiol. 43, 551–555.
Kwon-Chung, K.J. and S.J. Kim. 1974. A second heterothallic Aspergillus. Mycologia 66, 628–638.
Latge, J.P., P.E. Verweij, and S. Bretagne. 2000. Molecular typing of Aspergillus fumigatus. pp. 471–482. In R.A. Samson and J.I. Pitt (eds.), Integration of modern taxonomic methods for Penicillium and Aspergillus classification. Harwood Academic publisher, Amsterdam, the Netherlands.
Leslie, L.E., B. Flannigan, and L.J.R. Milner. 1988. Morphological studies on clinical isolates of Aspergillus fumigatus. J. Med. Vet. Mycol. 26, 335–341.
Lonial, S., L. Williams, G. Carum, M. Ostrowski, and P. McCarthy. 1997. Neosartorya fischeri; an invasive fungal pathogen in an allogenic bone marrow transplant patient. Bone Marrow Transpl. 19, 753–755.
Padhye, A.A., J.H. Godfrey, F.W. Chandler, and S.W. Peterson. 1994. Osteomyelitis caused by Neosartorya pseudofischeri. J. Clin. Microbiol. 32, 2832–2836.
Rinyu, E., J. Varga, and L. Ferenczy. 1995. Phenotypic and genotypic analysis of variability in Aspergills fumigatus. J. Clin. Microbiol. 33, 2567–2575.
Samson, R.A., S.B. Hong, S.W. Peterson, J.C. Frisvad, and J. Varga. 2007. Polyphasic taxonomy of Aspergillus section Fumigati and its teleomorph Neosartorya. Stud. Mycol. 59, 147–207.
Summerbell, R.C., L.D. Repentigney, C. Chartrand, and G. St. Germain. 1992. Graft-related endocarditis caused by Neosartorya fischeri var. spinosa. J. Clin. Microbiol. 30, 1580–1582.
Takada, M., Y. Horie, and P. Abliz. 2001. Two new heterothallic Neosartorya from African soil. Mycoscience 42, 361–367.
Udagawa, S., H. Tsubouchi, and N. Toyazaki. 1996. Isolation and identification of Neosartorya species from house dust as hazardous indoor pollutants. Mycoscience 37, 217–222.
Van Belkum, A., W.G.V. Quint, B.E. DePauw, W.J.G. Melchers, and J.F. Meis. 1993. Typing of Aspergilus species and Aspergillus fumigatus isolates by interrepeat polymerase chain reaction. J. Clin. Microbiol. 31, 2502–2505.
Varga, J., B. Toth, K. Rigo, F. Debets, and Z. Kozakiewicz. 2000a. Genetic variability within the Aspergillus viridinutans species. Folia Microbiol. 45, 423–428.
Varga, J., Z. Vida, B. Toth, F. Debets, and Y. Horie. 2000b. Phylogenetic analysis of newly described Neosartorya species. Antonie van Leeuwenhoek 77, 235–239.
Wang, L., K. Uokoyama, M. Miyaji, and K. Nishimura. 2000. Mitochondrial cytochrome b gene analysis of Aspergillus fumigatus and related species. J. Clin. Microbiol. 38, 1352–1358.
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Hong, SB., Kim, DH., Park, IC. et al. Re-identification of Aspergillus fumigatus sensu lato based on a new concept of species delimitation. J Microbiol. 48, 607–615 (2010). https://doi.org/10.1007/s12275-010-0084-z
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DOI: https://doi.org/10.1007/s12275-010-0084-z