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Catalase-imprinted Fe3O4/Fe@fibrous SiO2/polydopamine nanoparticles: An integrated nanoplatform of magnetic targeting, magnetic resonance imaging, and dual-mode cancer therapy

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Abstract

Recent advances in the research on the molecular mechanism of cell death and methods for preparation of nanomaterials make the integration of various therapeutic approaches, targeting, and imaging modes into a single nanoscale complex a new trend for the development of future nanotherapeutics. Hence, a novel ellipsoidal composite nanoplatform composed of a magnetic Fe3O4/Fe nanorod core (~120 nm) enwrapped by a catalase (CAT)-imprinted fibrous SiO2/ polydopamine (F-SiO2/PDA) shell with thickness 70 nm was prepared in this work. In vitro experiments showed that the Fe3O4/Fe@F-SiO2/PDA nanoparticles can selectively inhibit the bioactivity of CAT in tumor cells by the molecular imprinting technique. As a result, the H2O2 level in tumor cells was elevated dramatically. At the same time, the Fe3O4/Fe core released Fe ions to catalyze the conversion of H2O2 to ·OH in tumor cells. Eventually, the concentration of ·OH in tumor cells rapidly rose to a lethal level thus triggering apoptosis. Combined with the remarkable near-infrared light (NIR) photothermal effect of the CAT-imprinted PDA layer, the Fe3O4/Fe@F-SiO2/PDA nanoparticles can effectively kill MCF-7, HeLa, and 293T tumor cells but are not toxic to nontumor cells. Furthermore, these nanoparticles show good capacity for magnetic targeting and suitability for magnetic resonance imaging (MRI). Therefore, the integrated multifunctional nanoplatform opens up new possibilities for high-efficiency visual targeted nonchemo therapy for cancer.

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Acknowledgements

We thank Prof. Zhishen Ge of the Department of Polymer Science and Engineering of USTC, Prof. Yu Zhao of Department of Plastic Surgery, The First Affiliated Hospital of Anhui Medical University, and Prof. Yinfeng Qian of Department of Radiology, The First Affiliated Hospital of Anhui Medical University for their kind help in providing the 808 nm semiconductor laser device, adipose-derived stem cells, and the instruction of MRI testing, respectively. This work was supported by the National Natural Science Foundation of China (Nos. 51103143, 51173175, 51473152, and 51573174), the Fundamental Research Funds for the Central Universities (Nos. WK2060200012 and WK3450000001).

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Authors and Affiliations

  1. CAS Key Laboratory of Soft Matter Chemistry, Department of Polymer Science and Engineering, University of Science and Technology of China, Hefei, 230016, China

    Jinxing Chen, Shan Lei, Kun Zeng, Mozhen Wang & Xuewu Ge

  2. Interdisciplinary Research Centre in Biomedical Materials (IRCBM), COMSATS Institute of Information Technology, Lahore, 54000, Pakistan

    Anila Asif

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  1. Jinxing Chen
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  2. Shan Lei
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  3. Kun Zeng
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  4. Mozhen Wang
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  6. Xuewu Ge
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Correspondence to Mozhen Wang or Xuewu Ge.

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Catalase-imprinted Fe3O4/Fe@fibrous SiO2/polydopamine nanoparticles: An integrated nanoplatform of magnetic targeting, magnetic resonance imaging, and dual-mode cancer therapy

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Chen, J., Lei, S., Zeng, K. et al. Catalase-imprinted Fe3O4/Fe@fibrous SiO2/polydopamine nanoparticles: An integrated nanoplatform of magnetic targeting, magnetic resonance imaging, and dual-mode cancer therapy. Nano Res. 10, 2351–2363 (2017). https://doi.org/10.1007/s12274-017-1431-8

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  • DOI: https://doi.org/10.1007/s12274-017-1431-8

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