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Three-dimensional multimodal sub-diffraction imaging with spinning-disk confocal microscopy using blinking/fluctuating probes

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Abstract

Three-dimensional imaging cannot be achieved easily using previously developed localization super-resolution techniques. Here, we present a three-dimensional multimodal sub-diffraction imaging technique with spinning-disk (SD) confocal microscopy called 3D-MUSIC, which not only has all the advantages of SD confocal microscopy, such as fast imaging speed, high signal-to-noise ratio, and optical-sectioning capability, but also extends its spatial resolution limit along all three dimensions. Both axial and lateral resolution can be improved simultaneously by virtue of the blinking/fluctuating nature of modified fluorescent probes, exemplified with the quantum dots. Further, super-resolution images with dual modality can be obtained through super-resolution optical fluctuation imaging (SOFI) and bleaching/blinking-assisted localization microscopy (BaLM). Therefore, fast super-resolution imaging can be achieved with SD-SOFI by capturing only 100 frames while SD-BaLM yields high-resolution imaging.

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Authors and Affiliations

  1. Department of Biomedical Engineering, College of Engineering, Peking University, Beijing, 100871, China

    Xuanze Chen, Zhiping Zeng, Hening Wang & Peng Xi

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  1. Xuanze Chen
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  2. Zhiping Zeng
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  4. Peng Xi
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Correspondence to Peng Xi.

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These authors contributed equally to this work.

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Chen, X., Zeng, Z., Wang, H. et al. Three-dimensional multimodal sub-diffraction imaging with spinning-disk confocal microscopy using blinking/fluctuating probes. Nano Res. 8, 2251–2260 (2015). https://doi.org/10.1007/s12274-015-0736-8

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  • DOI: https://doi.org/10.1007/s12274-015-0736-8

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