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Ajoene restored behavioral patterns and liver glutathione level in morphine treated C57BL6 mice

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Abstract

Oxidative stress exacerbates drug dependence induced by administration of opiate analgesics such as morphine-induced tolerance and physical dependence associated with the reduction in hepatic glutathione (GSH) level. Ajoene obtained from garlic (Allium sativum L.) has been reported for anti-tumorigenic, anti-oxidative and neuroprotective properties, however, little is known about its effect on morphine-induced dependence. Therefore, this study aimed at the effect of ajoene on physical and/or psychological dependence and liver GSH content in morphine-treated mice. Conditioned place preference (CPP) test and measurement of morphine withdrawal syndrome were performed in C57BL6 mice for behavioral experiments. Thereafter, mice were sacrificed for measurement of serum and liver GSH levels. Ajoene restored CPP and naloxone-precipitated jumping behavior in mice exposed to morphine. Moreover, the reduced level of liver GSH content in morphine treated mice was back to normal after ajoene administration. Taken together, ajoene improved behavioral patterns in mice exposed to morphine suggesting its potential therapeutic benefit against morphine-induced dependence.

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Acknowledgments

This research was supported by the National Research Foundation of Korea (NRF) Grant funded by the Ministry of Science, ICT and Future Planning (SO, MRC, 2010-0029355; JR, 2011-0030074) and Ministry of Food and Drug Safety Grant (14182MFDS979).

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Authors and Affiliations

  1. Department of Molecular Medicine and Tissue Injury Defense Research Center, School of Medicine, Ewha Womans University, Anyanchon-ro 1071, Yangcheon-Ku, Seoul, 158-710, Republic of Korea

    Sergiy Oliynyk, Yeonju Lee, Jieun Kim, Kyunghwa Yun & Seikwan Oh

  2. Research Center for Cell Fate Control, College of Pharmacy, Sookmyung Women’s University, Seoul, Republic of Korea

    Raok Jeon & Jae-Ha Ryu

  3. Pharmacological Research Division, National Institute of Food and Drug Safety Evaluation, Ministry of Food and Drug Safety, Cheongju, 363-700, Republic of Korea

    Jaesuk Yun

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  1. Jaesuk Yun
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  2. Sergiy Oliynyk
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Correspondence to Seikwan Oh.

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Yun, J., Oliynyk, S., Lee, Y. et al. Ajoene restored behavioral patterns and liver glutathione level in morphine treated C57BL6 mice. Arch. Pharm. Res. 40, 106–111 (2017). https://doi.org/10.1007/s12272-016-0773-1

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  • DOI: https://doi.org/10.1007/s12272-016-0773-1

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