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In vitro-Zytotoxizitätsanalyse mit humanen Primärhepatozyten

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Abstract

Typically in vitro screening has incorporated primary hepatocytes cultured in a two dimensional (2D) format. However, when cultured and studied in this fashion they rapidly lose their key functions and dedifferentiate over the course of only a few days. Here we present data demonstrating the differences in response between human primary cells cultured in 2D and a new 3D cell culture system which has the benefits of a collagen hydrogel with tissue-like properties.

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Authors and Affiliations

  1. BioTek Instruments, Inc., Winooski, VT, USA

    Brad Larson

  2. TAP Biosystems, Royston, UK

    Tim Moeller

  3. Bioreclamation IVT, Baltimore, MD, USA

    Grant Cameron

  4. BioTek Instruments GmbH, Kocherwaldstraße 34, D-74177, Bad Friedrichshall, Deutschland

    Joël Mailliet

Authors
  1. Brad Larson
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  2. Tim Moeller
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  3. Grant Cameron
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  4. Joël Mailliet
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Correspondence to Joël Mailliet.

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Larson, B., Moeller, T., Cameron, G. et al. In vitro-Zytotoxizitätsanalyse mit humanen Primärhepatozyten. Biospektrum 20, 776–779 (2014). https://doi.org/10.1007/s12268-014-0519-x

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  • DOI: https://doi.org/10.1007/s12268-014-0519-x

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