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Circulating exosome-derived miR-122-5p is a novel biomarker for prediction of postoperative atrial fibrillation

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Abstract

Postoperative atrial fibrillation (POAF) is a frequent complication associated with increased periprocedural mortality and morbidity after cardiac surgery. Our study aimed to identify the difference in exosomal miRNA and further explore its role in the diagnosis of POAF. First, the differentially expressed miRNAs (DEMs) were obtained by high-throughput RNA sequencing. Second, the DEMs target genes were put into gene ontology (GO) and KEGG pathway analysis. Third, real-time quantification PCR (RT-qPCR) was used to verify the DEMs. Finally, we revealed 23 DEMs in POAF patients. Furthermore, analysis of gene function revealed that DEMs may affect atrial structure through many signaling pathways. We also found that miR-122-5p was up-regulated in POAF patients, but there are no significant changes in miR-191-5p, miR-181a-5p, miR-155-5p and miR-151a-5p. Our study revealed that exosomal miRNAs exert enormous potential in evaluating the severity or prognostic of POAF.

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Acknowledgements

This study was supported by the National Natural Science Foundation of China (No. 81770320, 82170311) to J. Wang.

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Authors and Affiliations

  1. Department of Cardiovascular Surgery, Beijing Anzhen Hospital, Capital Medical University, Beijing, 100029, China

    Chen Bai, Yichen Zhao, Qing Ye, Cheng Zhao, Yang Liu & Jiangang Wang

  2. School of Nursing, Capital Medical University, Beijing, 100069, China

    Yisi Liu

Authors
  1. Chen Bai
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  2. Yisi Liu
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  3. Yichen Zhao
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  4. Qing Ye
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  5. Cheng Zhao
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  6. Yang Liu
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  7. Jiangang Wang
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Contributions

C.B. and Y.L. contributed to writing—original draft preparation. C.B., Y.Z., Q.Y. and C.Z. contributed to software, data curation. C.B. and Y.L. contributed to resources, validation. J.W. contributed to writing—reviewing and editing, supervision. All authors approved the manuscript for publication.

Corresponding author

Correspondence to Jiangang Wang.

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Ethics Statement

All procedures performed in the study have been approved by the Ethics Committee of Beijing Anzhen Hospital, and the informed consent of all individuals participating in the study or their guardians has been obtained.

Conflicts of Interests

The authors declare that they have no competing interests.

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Associate Editor Junjie Xiao oversaw the review of this article.

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Bai, C., Liu, Y., Zhao, Y. et al. Circulating exosome-derived miR-122-5p is a novel biomarker for prediction of postoperative atrial fibrillation. J. of Cardiovasc. Trans. Res. 15, 1393–1405 (2022). https://doi.org/10.1007/s12265-022-10267-4

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  • DOI: https://doi.org/10.1007/s12265-022-10267-4

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