Abstract
Estrogen is a vascular protection factor and plays a protective role in the pathogenesis of gender differences in cardiovascular diseases. This study was to address the possible mechanisms that may explain the relationship between estradiol configuration—17β-estradiol (E2) and ventricular remodeling. Here, we show that a total of 1499 LncRNAs and 680 mRNAs significantly differently expressed were identified. This result indicates that estradiol has a global role in regulating heart gene expression profiles in female mice. Go and Pathway functional cluster analysis showed that the antagonism of E2 on cardiac remodeling and AngII-induced pathological changes in female mice may be related to physiological processes such as circadian rhythm disorder and ion channel dysfunction.
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Abbreviations
- ACE:
-
angiotensin-converting enzyme
- Ang II:
-
angiotensin II
- AT1R:
-
angiotensin II type1 receptor
- BP:
-
blood pressure
- CVF:
-
collagen volume fraction
- DBP:
-
diastolic blood pressure
- E2 :
-
17β-estradiol
- ERK:
-
extracellular signal-regulated kinase
- FC:
-
fold change
- FGFR:
-
fibroblast growth factor receptor
- GO:
-
gene ontology
- HE:
-
hematoxylin-eosin
- HRT:
-
hormone replacement therapy
- LncRNA:
-
long noncoding RNA
- NADPH:
-
nicotinamide adenine dinucleotide phosphate
- NOX:
-
anti-NADPH oxidase
- NPNT:
-
renal surface protein
- OVX:
-
ovariectomy
- PBS:
-
phosphate-buffered saline
- PI3K/Akt:
-
phosphoinositol 3-kinase/serine/threonine kinase
- PVCA/LA:
-
perivascular collagen area/lumen area
- qRT-PCR:
-
quantificational real-time polymerase chain reaction
- RAAS:
-
renin-angiotensin-aldosterone system
- ROS:
-
reactive oxygen species
- SBP:
-
systolic blood pressure
- Sham:
-
sham operation
- TDLVMC:
-
transverse section of left ventricular myocytes
- TGF-β1:
-
transforming growth factor-β1
- T-SOD:
-
total superoxide dismutase
- VSMC:
-
vascular smooth muscle cell
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Funding
This work was supported by the National Natural Science Foundation of China (No. 81800363), the Provincial Natural Science Foundation of Fujian (No. 2018J01240), the Fujian Provincial Medical Innovation Project (No. 2018-CX-4), the Funding Scheme for Young and Middle-aged Talents Development Projects (No. 2018-XQN-24) in Fujian Provincial Health Family Planning Commission, and the Joint Fund for Research and Construction of High-Level Hospitals in Fujian Provincial Hospital (No. 2017LHJJ03).
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ZJK and CH conceived and designed the study. ZJK and WH performed the experiments. ZJK wrote the paper. CH reviewed and edited the manuscript. All authors read and approved the manuscript.
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Zhu, J., Wang, H. & Chen, H. Effects of Estrogen on Cardiac mRNA and LncRNA Expression Profiles in Hypertensive Mice. J. of Cardiovasc. Trans. Res. 14, 706–727 (2021). https://doi.org/10.1007/s12265-020-09990-7
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DOI: https://doi.org/10.1007/s12265-020-09990-7