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Serum miR-92a-3p as a New Potential Biomarker for Diagnosis of Kawasaki Disease with Coronary Artery Lesions

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Abstract

Recent studies have suggested that serum microRNAs (miRNAs) are novel biomarkers for many cardiovascular diseases, but their role in Kawasaki disease (KD) is still unclear. We demonstrated that serum miR-92a-3p levels were significantly higher in children with KD compared with children with fever and controls (both P < 0.05). When the disease recovered, miR-92a-3p levels returned to those of controls. Clinical and pathological data showed that high levels of miR-92a-3p were significantly associated with coronary artery lesions (CALs). Analysis of the receiver operating characteristic (ROC) curve showed that serum miR-92a-3p had a sensitivity of 81.8% and a specificity of 66.7% for distinguishing KD with CALs from KD without CALs. The area under the curve was 0.816 (P < 0.05, 95% CI 0.669–0.962). Therefore, the miRNA miR-92a-3p may be used as a potential biomarker for diagnosis of KD and KD with coronary artery lesions.

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Abbreviations

AUC:

Area under the curve

CALs:

Coronary artery lesions

CRP:

C-reactive protein

ESR:

Erythrocyte sedimentation rate

FGF:

Fibroblast growth factor

HUVECs:

Human umbilical vein endothelial cells

KD:

Kawasaki disease

miRNAs:

MicroRNAs

NT-proBNP:

N-terminal pro natriuretic peptide type B

RT-PCR:

Real-time reverse transcription-PCR

WBC:

White blood cell

ROC:

Receiver operating characteristic

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Acknowledgements

The technical assistance of Jingjing Zeng is greatly acknowledged.

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    Authors and Affiliations

    1. Children’s Heart Center, the Second Affiliated Hospital & Yuying Children’s Hospital, Institute of Cardiovascular Development and Translational Medicine, Wenzhou Medical University, Wenzhou, 325027, Zhejiang, China

      Xing Rong, Lanlan Hong, Lulu Pan, Huixian Qiu, Rongzhou Wu & Maoping Chu

    2. Department of Second Pediatrics, The People’s Hospital of Langfang City, Langfang, 065000, Hebei, China

      Lianhong Jia

    3. Department of Microbiology and Immunology, Wenzhou Medical University, Wenzhou, 325035, Zhejiang, China

      Xiangyang Xue

    4. Department of Biomedical Engineering, School of Medicine, The University of Alabama at Birmingham, Birmingham, AL, 35233, USA

      Chunxiang Zhang

    5. Department of Pediatrics, The First Affiliated Hospital of Wenzhou Medical University, Wenzhou, 325015, Zhejiang, China

      Jiacheng Lu & Zengyou Jin

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    1. Xing Rong
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    Correspondence to Rongzhou Wu or Maoping Chu.

    Ethics declarations

    All procedures followed were in accordance with the ethical standards of the Second Affiliated Hospital & Yuying Children’s Hospital of Wenzhou Medical Ethics Committee. Informed consent was obtained from all patients for being included in the study.

    Funding

    This work was supported by the Zhejiang Provincial Natural Science Foundation of China (LY12H02003, LQ15H020006), the Zhejiang Provincial Medical and Health Science and Technology Plan (2012ZDA035), the Science and Technology Department of Zhejiang Province (2015C33163), and the Scientific Research Foundation of Wenzhou (Y20140051).

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    The authors declare that they have no conflict of interest.

    Ethical approval

    All procedures performed in studies involving human participants were in accordance with the ethical standards of the institutional and/or national research committee and with the 1964 Helsinki Declaration and its later amendments or comparable ethical standards.

    This article does not contain any studies with animals performed by any of the authors.

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    Xing Rong and Lianhong Jia contributed equally to this work.

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    Rong, X., Jia, L., Hong, L. et al. Serum miR-92a-3p as a New Potential Biomarker for Diagnosis of Kawasaki Disease with Coronary Artery Lesions. J. of Cardiovasc. Trans. Res. 10, 1–8 (2017). https://doi.org/10.1007/s12265-016-9717-x

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