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Sevoflurane exposure in 7-day-old rats affects neurogenesis, neurodegeneration and neurocognitive function

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Abstract

Objective

Sevoflurane is widely used in pediatric anesthesia and former studies showed that it causes neurodegeneration in the developing brain. The present study was carried out to investigate the effects of sevoflurane on neurogenesis, neurodegeneration and behavior.

Methods

We administered 5-bromodeoxyuridine, an S-phase marker, before, during, and after 4 h of sevoflurane given to rats on postnatal day 7 to assess dentate gyrus progenitor proliferation and Fluoro-Jade staining for degeneration. Spatial reference memory was tested 2 and 6 weeks after anesthesia.

Results

Sevo-flurane decreased progenitor proliferation and increased cell death until at least 4 days after anesthesia. Spatial reference memory was not affected at 2 weeks but was affected at 6 weeks after sevoflurane administration.

Conclusion

Sevoflurane reduces neurogenesis and increases the death of progenitor cells in developing brain. This might mediate the late-onset neurocognitive outcome after sevoflurane application.

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Authors and Affiliations

  1. Department of Anesthesiology, Zhongshan Hospital, Fudan University, Shanghai, 200032, China

    Fang Fang, Zhanggang Xue & Jing Cang

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  1. Fang Fang
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  2. Zhanggang Xue
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  3. Jing Cang
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Correspondence to Jing Cang.

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Fang, F., Xue, Z. & Cang, J. Sevoflurane exposure in 7-day-old rats affects neurogenesis, neurodegeneration and neurocognitive function. Neurosci. Bull. 28, 499–508 (2012). https://doi.org/10.1007/s12264-012-1260-4

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  • DOI: https://doi.org/10.1007/s12264-012-1260-4

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