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Physicochemical characterization of gelatin-immobilized, acrylic acid-bacterial cellulose nanofibers as cell scaffolds using gamma-irradiation

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Abstract

Bacterial cellulose (BC) has been shown to have a high-burst pressure, high-water contact, and ultrafine highly nanofibrous structure similar with that in a natural extracellular matrix (ECM). In the present study, we developed a BC-based functional scaffold for tissue engineering using radiation technology. BC was generated by Gluconacetobacter hansenii TL-2C. Acrylic acid (AAc) was grafted onto BC surfaces under aqueous conditions using gamma-ray irradiation. The characterization of the scaffold was performed by scanning electron microscopy, ATR-FTIR spectroscopy, a toluidine blue O assay, and 2,4,6,-trinitro-benzensulfonic acid assay. AAc was grafted on the BC under gamma-ray irradiation. Gelatin was chemically conjugated on the AAc-BC scaffolds through EDC chemistry. The morphology of the modified BC nanofibers did not change, while representative features of AAc and gelatin were maintained. The adhesion and spreading of human mesenchymal stem cells was improved on the gelatin-AAc-BC nanofibers compared to unmodified BC and AAc-BC nanofibers. Our results suggest that gelatin-immobilized BC nanofiber scaffolds can be a promising way to fabricate three-dimentional, nanofibrous scaffolds that accelerate cell behavior for biomedical applications.

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Authors and Affiliations

  1. Research Division for Industry & Environment, Korea Atomic Energy Research Institute, Jeongeup, 580-185, Korea

    Youn-Mook Lim, Sung In Jeong, Young Min Shin, Jong-Seok Park, Hui-Jeong Gwon, Young-Chang Nho, Sung-Jun An, Jong-Bae Choi & Jin-Oh Jeong

  2. Department of Radiation Science and Technology, Chonbuk National University, Jeonju, 561-756, Korea

    Sung-Jun An

  3. Department of Prosthodontics, School of Dentistry, Pusan National University, Dental Research Institute, Yangsan, 626-870, Korea

    Jae-Won Choi

Authors
  1. Youn-Mook Lim
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  2. Sung In Jeong
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  3. Young Min Shin
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  4. Jong-Seok Park
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  5. Hui-Jeong Gwon
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  6. Young-Chang Nho
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  7. Sung-Jun An
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  8. Jong-Bae Choi
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  9. Jin-Oh Jeong
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  10. Jae-Won Choi
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Correspondence to Youn-Mook Lim.

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Lim, YM., Jeong, S.I., Shin, Y.M. et al. Physicochemical characterization of gelatin-immobilized, acrylic acid-bacterial cellulose nanofibers as cell scaffolds using gamma-irradiation. Biotechnol Bioproc E 20, 942–947 (2015). https://doi.org/10.1007/s12257-015-0175-0

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  • DOI: https://doi.org/10.1007/s12257-015-0175-0

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