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Development and Evaluation of a Universal and Supersensitive NS1-Based Luciferase Immunosorbent Assay to Detect Zika Virus-Specific IgG

  • RESEARCH ARTICLE
  • Published:
Virologica Sinica

Abstract

Zika virus (ZIKV) causes rash, moderate fever, conjunctivitis, and arthralgia, and has serious connection with neurological complications; therefore, it is a major threat to public health. A rapid and supersensitive method for detecting anti-ZIKV antibodies in humans and animals is thus urgently required. Here, we report an NS1-based luciferase immunosorbent assay (LISA), developed to detect ZIKV-specific IgG. Fusion proteins including a reporter Nano-luciferase (NLuc) and various fragments of ZIKV NS1 protein were expressed in 293 T cells. LISA was performed using the above cell lysates containing the expressed fusion proteins. Sample panels of humans and animals infected with ZIKV were examined for sensitivity of LISA, relative to those of ZIKV RT-PCR, commercial NS1-based ELISA, and micro-neutralization (MN) assays. Specificity and potential cross-reactivity were also evaluated using various convalescent serum samples derived from patients infected with dengue virus (DENV), Japanese encephalitis virus (JEV), and hepatitis C virus (HCV). Results indicated the optimal antigenic domain for anti-ZIKV IgG detection was located within 172–352 amino acids (aa) of ZIKV NS1 protein. NS1-based LISA performs better than commercial ELISA in anti-ZIKV IgG detection. LISA was shown to be at least fourfold more sensitive than commercial ELISA, and could detect anti-ZIKV IgG in various animal hosts without the need of species-specific labeled antibody. This novel assay is potentially useful for the rapid and sensitive detection of anti-ZIKV IgG in human and animal samples.

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Acknowledgements

This work was supported by the following grants: the National Key Research and Development Program of China (2016YFD0500301), the National Major Project for Control and Prevention of Infectious Disease in China (2018ZX10101002 and 2017YFC1200503), the Bureau of Science and Information Technology of Guangzhou Municipality, China (201604020011, 201704020219), and National Key R&D Program of China (2018ZX10732401). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. We thank all the staffs from respective authorities of China for providing all samples.

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Author notes

  1. Tianyu Wang, Ying Zhan, De Wu and Zhihai Chen have contributed equally to this work.

Authors and Affiliations

  1. Guangdong Provincial Key Laboratory of Tropical Disease Research, Department of Epidemiology, School of Public Health, Southern Medical University, Guangzhou, 510515, China

    Tianyu Wang & Shixing Tang

  2. NHC Key Laboratory of Biosafety, National Institute for Viral Disease Control and Prevention, China CDC, Beijing, 102206, China

    Tianyu Wang, Ying Zhan, Wei Wu, Yao Deng, Wenling Wang & Wenjie Tan

  3. Key Laboratory for Repository and Application of Pathogenic Microbiology, Research Center for Pathogens Detection Technology of Emerging Infectious Diseases, Guangdong Provincial Center for Disease Control and Prevention, Guangzhou, 511430, China

    De Wu

  4. The National Clinical Key Department of Infectious Disease, Beijing Ditan Hospital, Capital Medical University, Beijing, 100015, China

    Zhihai Chen

Authors
  1. Tianyu Wang
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  2. Ying Zhan
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  3. De Wu
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  5. Wei Wu
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Contributions

ST and WT conceived and designed experiments. TW, YZ, YD and W Wu performed the experiments. TW, W Wang and WT analyzed the data. DW, ZC, W Wu and W Wang contributed reagents/material/analysis tools. TW, ST and WT wrote the manuscript.

Corresponding authors

Correspondence to Wenjie Tan or Shixing Tang.

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The authors declare that they have no conflict of interest.

Animal and Human Rights Statement

A total of 151 samples from human and animal were used in this study, which was approved by the Ethics Committee of Southern Medical University (SMU No. 20160428) and the National Institute for Viral Disease Control and Prevention of China (IVDC No. 2016072501), as well as the waiver of the Informed Consents.

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Wang, T., Zhan, Y., Wu, D. et al. Development and Evaluation of a Universal and Supersensitive NS1-Based Luciferase Immunosorbent Assay to Detect Zika Virus-Specific IgG. Virol. Sin. 35, 93–102 (2020). https://doi.org/10.1007/s12250-019-00160-x

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  • DOI: https://doi.org/10.1007/s12250-019-00160-x

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