Abstract
To obtain the helper plasmids for a reverse genetics system of rabies virus, the cDNAs of the complete open reading frames of the N, P, G, and L genes of rabies street virus stain HN10 were each cloned into expression vector pVAX1, These four plasmids were identified by restriction enzyme digestion and gene sequencing. The plasmid encoding the N protein was selected to determine the expression effect of these plasmids in NA cells. The results showed that the helper plasmids for a reverse genetics system of rabies street virus strain HN10 had been successfully constructed.
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Foundation items: National High Technology Research and Development Program of China (2006AA02Z110, 2007 AA02Z402); Major Program of the National Natural Science Foundation of China (30630049).
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Ming, Pg., Huang, Y., Tang, Q. et al. Construction and identification of the helper plasmids for reverse genetic system of rabies virus street strain. Virol. Sin. 24, 559–565 (2009). https://doi.org/10.1007/s12250-009-3039-y
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DOI: https://doi.org/10.1007/s12250-009-3039-y