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The Origin of Southeastern Asian Triploid Edible Canna (Canna discolor Lindl.) Revealed by Molecular Cytogenetical Study1

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The Origin of Southeastern Asian Triploid Edible Canna ( Canna discolor Lindl.) Revealed by Molecular Cytogenetical Study. Canna discolor Lindl. (Cannaceae), commonly known as edible canna, is often cultivated in Southeastern Asia for its starchy rhizomes. Based on morphological and karyological features, it is thought to be an allotriploid plant originated from hybridization between the closely allied C. coccinea Mill., C. patens Roscoe, C. plurituberosa T. Koyama & Nb. Tanaka, C. speciosa Roscoe, or C. indica L. In this study, to clarify the origin of triploid edible canna, physical mapping of 5S and 18S rDNA probes in C. discolor and its closely related five putative parental species was conducted. Fluorescence in situ hybridization (FISH) technique provided a useful chromosomal marker for discriminating among the diploid putative parental Canna species, and supported the hybrid origin of C. discolor between C. indica var. indica and C. plurituberosa.

分子細胞遺伝学的研究で明らかになった東南アジアの三倍体食用カンナの起源.食用カンナは,中南米を原産とするカンナ科の大型多年生草本で,東南アジアにも伝播してデンプンを含み肥大する根茎が地域的に食用に利用されている.　東南アジアで一般に食用カンナと呼ばれている三倍体種C. discolor(2n = 27)は,核型的な特徴から異質倍数性起源であることが示唆された.　本研究では, 現在までの形態学的および核型研究からC. discolorはC. indicaとその近縁4分類群(C. patens, C. plurituberosa, C. speciosa, C. coccinea)との交雑起源であるという仮説を立て,それを検証するため, 5S及び18S ribosomal RNA遺伝子座の同時検出を行った.その結果, FISHのデータからC. discolorは,C. indicaの非還元配偶子(2n)とC. plurituberosaの減数配偶子(n)の交雑に由来する異質倍数体であると考えられる.

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Acknowledgments

The authors thank Y. Hayami and M. Hamaguchi for taking care of our living materials. We also wish to thank Prof. David Bufford, Harvard University, for reviewing the English manuscript. This research is partially supported by Grant-in-Aid from Japanese Society for the Promotion of Science (to N. Tanaka) and Grant-in-Aid from Kochi Prefectural Government.

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Authors and Affiliations

  1. College of Bioresource Sciences, Nihon University, 1866 Kameino, Fujisawa, Kanagawa, 252-0880, Japan

    Hideyuki Matoba & Hiroshi Uchiyama

  2. Kanagawa Prefectural Museum of Natural History, 499 Iryuda, Odawara, Kanagawa, 250-0031, Japan

    Hideyuki Matoba

  3. Kochi Prefectural Makino Botanical Garden, 4200-6, Godaisan, Kochi, 781-8125, Japan

    Nobuyuki Tanaka & Tetsuo Koyama

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  1. Hideyuki Matoba
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Correspondence to Nobuyuki Tanaka.

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Matoba, H., Tanaka, N., Uchiyama, H. et al. The Origin of Southeastern Asian Triploid Edible Canna (Canna discolor Lindl.) Revealed by Molecular Cytogenetical Study1 . Econ Bot 65, 308–314 (2011). https://doi.org/10.1007/s12231-011-9167-y

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