Summary
A pilot study of leaf outline morphometrics was carried out on populations of five species of Heteropsis located in the Reserva Florestal Adolpho Ducke near Manaus, Amazonas, Brazil: H. flexuosa (Kunth) G. S. Bunting, H. macrophylla A. C. Sm., H. spruceana Schott, H. steyermarkii G. S. Bunting and H. tenuispadix G. S. Bunting. The aim of the study was to investigate quantitative methods for discriminating species within a local area based on vegetative morphology in a genus where fertile parts are often difficult to find in the field; this study focussed on leaf outline shape. Using digital images of 347 leaves, outlines were captured as coordinates using the TpsDig software and analysed using Elliptic Fourier Analysis (EFA). Principal Component Analysis (PCA) was used to reduce the 160 elliptic Fourier coefficient descriptors to a smaller number of independent shape variables corresponding to the principal component axes. The first nineteen shape variables (constrained by the smallest species sample, N = 20) were then subjected to Linear Discriminant Analysis (LDA) to find new axes that would discriminate the five species to the maximum extent. The first six shape variables were visualised by a reification procedure in order to illustrate their characteristic contribution to the total shape variation within all five species. The results showed that the mean shapes of all five species were significantly different, but shape variation within each species overlapped with the others. Percentage assignment of individuals to their correct species was encouragingly high given that only outline shape was used, but was not high enough overall to provide reliable identification. Elimination of one species (H. steyermarkii) using easily observed qualitative vegetative characters improved discrimination of the remaining four. The investigation of new approaches to identification is potentially valuable for conservation of natural populations — the root fibre of Heteropsis is extracted from primary forest and is a valuable non-timber forest product that forms the basis for an important local industry in Amazonia.
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References
Andrade, I. M. (2006). Estudos morfométricos e genêticos em populaces de duas espécies de Araceae em areas florestais do Brasil, especialmente no Ceará. Unpublished PhD thesis, Universidade Estadual de Feira de Santana (UEFS), Feira de Santana, Bahia.
____, Mayo, S. J., Kirkup, D. & Van den Berg, C. (2008). Comparative morphology of populations of Monstera (Araceae) from natural forest fragments in Northeast Brazil using elliptic Fourier Analysis of leaf outlines. Kew Bull. 63: 193 – 211.
____, ____, ____ & ____ (2010). Elliptic Fourier Analysis of leaf outline shape in forest fragment populations of Anthurium sinuatum and A. pentaphyllum (Araceae) from Northeast Brazil. Kew Bull. 65: 3 – 20.
Claude, J. (2008). Morphometrics with R. Springer, New York.
Durigan, C. C. & Castillo, C .V. (2004). O extrativismo de cipós (Heteropsis spp., Araceae) no Parque Nacional do Jaú. In: S. H. Borges, S. Iwanaga, C. C. Durigan & M. R. Pinheiro (eds), Janelas para a biodiversidade no Parque Nacional do Jaú: Uma estratégia para o estudo da biodiversidade na Amazônia, pp. 231 – 242. Fundação Vitória Amazônica, Manaus.
Fadiman, M. G. (2003). Fibres from the forest: Mestizo, Afro-Ecuadorian and Chachi Ethnobotany of Piquigua (Heteropsis ecuadorensis, Araceae) and Mocora (Astrocaryum standleyanum, Arecaceae) in Northwestern Ecuador. PhD thesis, Faculty of the Graduate School of the University of Texas, Austin.
Hammer, Ø., Harper, D. A. T. & Ryan, P. D. (2001). PAST: Palaeontological Statistics software package for education and data analysis. Palaeontologia Electronica 4: 1 – 9. Available at http://folk.uio.no/ohammer/past.
Kimball, S. & Mattis, P. (2006). Gimp. Version 2.2.4. http://www.gimp.org.
Knab-Vispo, C., Hoffmann, B., Moermond, T. & Vispo, C. (2003). Ecological observations on Heteropsis spp. (Araceae) in southern Venezuela. Econ. Bot. 57: 345 – 353.
Lexer, C., Joseph, J., van Loo, M., Prenner, G., Heinze, B., Chase, M. W. & Kirkup, D. (2009). The use of digital image-based morphometrics to study the phenotypic mosaic in taxa with porous genomes. Taxon 58: 349 – 364.
Plotze, R. O., Falvo, M., Pádua, J. G., Bernacci, L. C., Vieira, M. L. C., Oliveira, G. C. X. & Bruno, O. M. (2005). Leaf shape analysis using the multiscale Minkowski fractal dimension, a new morphometric method: a study with Passiflora (Passifloraceae). Canad. J. Bot. 83: 287 – 301.
Plowden, C., Uhl, C. & De Assis Oliveira, F. (2003). The ecology and harvest potential of titica vine roots (Heteropsis flexuosa: Araceae) in the eastern Brazilian Amazon. Forest Ecol. Managem. 182: 59 – 73.
R Development Core Team (2008). R: a Language and Environment for Statistical Computing. R Foundation for Statistical Computing, Vienna. http://www.R-project.org.
Ray, T. S. (1990). Application of eigenshape analysis to second order leaf shape ontogeny in Syngonium podophyllum (Araceae). In: F. J. Rohlf & F. L. Bookstein (eds), Proceedings of the Michigan Morphometrics Workshop. Special Publication No. 2, pp. 201 – 213. The University of Michigan Museum of Zoology, Ann Arbor, Michigan.
____ (1992). Landmark eigenshape analysis: homologous contours: leaf shape in Syngonium (Araceae). Amer. J. Bot. 79: 69 – 76.
Ribeiro, J. E. L. S., Hopkins, M. J. G., Vicentini, A., Sothers, C. A., Costa, M. A. S., Brito, J. M., Souza, M. A. D., Martins, L. H. P., Lohmann, L. G., Assunção, P. A. C. L., Pereira, E. C., Silva, C. F., Mesquita, C. F. & Procópio, L. C. (1999). Flora da Reserva Ducke. INPA/DFID, Manaus.
Rohlf, F. J. (2004). TpsDig, Version 1.40. Digitizing Software. http://life.bio.sunysb.edu/morph/.
____ (2005). NTSYSpc: Numerical Taxonomy System ver. 2.20 d. Exeter Publishing, Ltd. Setauket, NY. http://www.exetersoftware.com/cat/ntsyspc/ntsyspc.html.
____ & Slice, D. E. (2003). BIOMstat, Statistical Software for Biologists, Version 3.30. Exeter Publishing, Ltd. Setauket, NY.
Slice, D. (1998). Morpheus et al. Software for Morphometric Research. http://life.bio.sunysb.edu/morph/morpheus/.
Soares, M. L. C. & Mayo, S. J. (1999). Araceae. In: J. E. L. S. Ribeiro, M. J. G. Hopkins, A. Vicentini, C. A. Sothers, M. A. S. Costa, J. M. Brito, M. A. D. Souza, L. H. P. Martins, L. G. Lohmann, P. A. C. L. Assunção, E. C. Pereira, C. F. Silva, C. F. Mesquita, & L. C. Procópio (eds), Flora da Reserva Ducke, pp. 672 – 687. INPA/DFID, Manaus.
Soares Morais, M. L. C. (2008). Sistemática e ecologia de Heteropsis Kunth (Araceae Juss.) com destaque especial nas espécies ocorrentes na Reserva Florestal Adolpho Ducke, Manaus – Amazonas, Brasil. PhD thesis, Instituto Nacional de Pesquisas da Amazônia / Universidade Federal das Amazonas, Manaus.
Yoshioka, Y., Iwata, H., Ohsama, R. & Ninomya, S. (2004). Analysis of petal shape variation of Primula sieboldii by elliptic Fourier descriptors and principal component analysis. Ann. Bot. 94: 657 – 664.
Acknowledgements
The first author (MLCS) thanks the following organisations for financial and infrastructural support: Coordenação de Pesquisas em Botânica do Instituto Nacional de Pesquisas da Amazônia (INPA), Fundação Botânica Margaret Mee, Margaret Mee Fellowships Programme of the Royal Botanic Gardens, Kew, and the Fundação O Boticário de Proteção à Natureza. We are very grateful to the curators of the following herbaria for making specimens available for our study: INPA, K, MG, MO, U.
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Appendix
Appendix
List of voucher specimens for populations sampled in this study. All specimens collected in Brazil, Amazonas State, Município de Manaus, Reserva Florestal Adolpho Ducke, 26 km north of the city of Manaus, 2°58'S, 59°57'W.
Heteropsis flexuosa (Kunth) G. S. Bunting: 21 Feb. 1995, M. L. C. Soares et al. 190 (INPA).
H. macrophylla A. C. Sm.: 14 Feb. 2002, M. L. C. Soares et al. 674 (INPA).
H. spruceana Schott: 16 April 1998, M. L. C. Soares et al. 415 (INPA).
H. steyermarkii G. S. Bunting: 22 Nov. 1995, M. L. C. Soares et al. 240 (INPA).
H. tenuispadix G. S. Bunting: 31 Jan. 2004, M. L. C. Soares et al. 565 (INPA).
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Soares, M.L.C., Mayo, S.J., Gribel, R. et al. Elliptic Fourier Analysis of leaf outlines in five species of Heteropsis (Araceae) from the Reserva Florestal Adolpho Ducke, Manaus, Amazonas, Brazil. Kew Bull 66, 463–470 (2011). https://doi.org/10.1007/s12225-011-9290-z
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DOI: https://doi.org/10.1007/s12225-011-9290-z