Abstract
Yeasts Cryptococcus humicola accumulated cadmium, cobalt, and iron (~ 50, 17, and 4% of the content in the medium, respectively) from the medium containing glucose, phosphate, and 2 mmol/L of metal salts. The effects of metal absorption on the levels of orthophosphate (Pi) and inorganic polyphosphate (polyP) varied for the metals under study. The levels of Pi and polyP increased in the case of cadmium and cobalt, respectively. In the case of iron, no changes in the levels of Pi and polyP were observed. Multiple DAPI-stained polyP inclusions were observed in the cytoplasm of cadmium-containing cells. The intensity of DAPI staining of the cell wall especially increased in case of cobalt and iron accumulation.
References
Andreeva N, Ryazanova L, Dmitriev V, Kulakovskaya T, Kulaev I (2014) Cytoplasmic inorganic polyphosphate participates in the heavy metal tolerance of Cryptococcus humicola. Folia Microbiol (Praha) 59(5):381–389. https://doi.org/10.1007/s12223-014-0310-x
Breus NA, Ryazanova LP, Dmitriev VV Kulakovskaya TV, Kulaev IS (2012) Accumulation of phosphate and polyphosphate by Cryptococcus humicola and Saccharomyces cerevisiae in the absence of nitrogen. FEMS Yeast Res 12(6):617–624. https://doi.org/10.1111/j.1567-1364.2012.00812.x
Fang Z, Chen Z, Wang S, Shi P, Shen Y, Zhang Y, Xiao J, Huang Z (2016) Overexpression of OLE1 enhances cytoplasmic membrane stability and confers resistance to cadmium in Saccharomyces cerevisiae. Appl Environ Microbiol 83(3):e02319–e02316. https://doi.org/10.1128/AEM.69.3.1499-1503.2003
Geva P, Kahta R, Nakonechny F, Aronov S, Nisnevitch M (2016) Increased copper bioremediation ability of new transgenic and adapted Saccharomyces cerevisiae strains. Environ Sci Pollut Res Int 23(19):19613–19625. https://doi.org/10.1007/s11356-016-7157-4
Kulaev IS, Vagabov VM, Kulakovskaya TV (2004) The biochemistry of inorganic polyphosphates. John Wiley and Sons Ltd, Chichester. https://doi.org/10.1002/0470858192
Kulakovskaya E, Kulakovskaya T (2014) Extracellular glycolipids of yeasts, 1st edn. Biodiversity, biochemistry, and prospects. Academic Press, Cambridge
Martin P, Van Mooy BA (2013) Fluorometric quantification of polyphosphate in environmental plankton samples: extraction protocols, matrix effects, and nucleic acid interference. Appl Environ Microbiol 79(1):273–281. https://doi.org/10.1128/AEM.02592-12
Pavlov E, Aschar-Sobbi R, Campanella M, Turner RJ, Gómez-García MR, Abramov AY (2010) Inorganic polyphosphate and energy metabolism in mammalian cells. J Biol Chem 285(13):9420–9428. https://doi.org/10.1074/jbc.M109.013011
Rosenfeld L, Reddi AR, Leung E, Aranda K, Jensen LT, Culotta CV (2010) The effect of phosphate accumulation on metal ion homeostasis in Saccharomyces cerevisiae. J Biol Inorg Chem 15(7):1051–1062. https://doi.org/10.1007/s00775-010-0664-8
Ryazanova L, Andreeva N, Kulakovskaya T, Valiakhmetov A, Yashin V, Vagabov V, Kulaev I (2011) The early stage of polyphosphate accumulation in Saccharomyces cerevisiae: comparative study by extraction and DAPI staining. Adv Biosci Biotechnol 2(04):293–297. https://doi.org/10.4236/abb.2011.24042
Serafim LS, Lemos OC, Levantesi C, Tandoi V, Santos H, Reis MA (2002) Methods for detection and visualization of intracellular polymers stored by polyphosphate-accumulating microorganisms. J Microbiol Meth 51(1):1–18. https://doi.org/10.1016/S0167-7012(02)00056-8
Singh P, Raghukumar C, Parvatkar RR, Mascarenhas-Pereira MB (2013) Heavy metal tolerance in the psychrotolerant Cryptococcus sp. isolated from deep-see sediments of Central Indian Basin. Yeast 30(3):93–101. https://doi.org/10.1002/yea.2943
Vadkertiová R, Sláviková E (2006) Metal tolerance of yeasts isolated from water, soil and plant environments. J Basic Microbiol 46(2):145–152. https://doi.org/10.1002/jobm.200510609
Vadkertiová R, Molnárová J, Lux A, Vaculík M, Lišková D (2016) Yeasts associated with an abandoned mining area in Pernek and their tolerance to different chemical elements. Folia Microbiol (Praha) 61(3):199–207. https://doi.org/10.1007/s12223-015-0424-9
Volesky B, Holan ZR (1995) Biosorption of heavy metals. Biotechnol Prog 11(3):235–250. https://doi.org/10.1021/bp00033a001
Vreulink JM, Stone W, Botha A (2010) Effects of small increases in copper levels on culturable basidiomycetous yeasts in low-nutrient soils. J Appl Microbiol 109(4):1411–1421. https://doi.org/10.1111/j.1365-2672.2010.04770.x.
Wang J, Chen C (2006) Biosorption of heavy metals by Saccharomyces cerevisiae: a review. Biotechnol Adv 24(5):427–451. https://doi.org/10.1016/j.biotechadv.2006.03.001
Xin S, Zeng Z, Zhou X, Luo W, Shi X, Wang Q, Deng H, Du Y (2017) Recyclable Saccharomyces cerevisiae loaded nanofibrous mats with sandwich structure constructing via bio-electrospraying for heavy metal removal. J Hazard Mater 324(Pt B):365–372. https://doi.org/10.1016/j.jhazmat.2016.10.070
Zheng XY, Wang XY, Shen YH, Lu X, Wang TS (2017) Biosorption and biomineralization of uranium(VI) by Saccharomyces cerevisiae—crystal formation of chernikovite. Chemosphere 175:161–169. https://doi.org/10.1016/j.chemosphere.2017.02.035
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The work was supported by the Russian Foundation for Basic Research (grant 16-04-00396).
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Kulakovskaya, T., Ryazanova, L., Zvonarev, A. et al. The biosorption of cadmium and cobalt and iron ions by yeast Cryptococcus humicola at nitrogen starvation. Folia Microbiol 63, 507–510 (2018). https://doi.org/10.1007/s12223-018-0583-6
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DOI: https://doi.org/10.1007/s12223-018-0583-6