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Cell viability of microencapsulated Bifidobacterium animalis subsp. lactis under freeze-drying, storage and gastrointestinal tract simulation conditions

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Abstract

The purpose of this study was to improve the survival of Bifidobacterium animalis subsp. lactis 10140 during freeze-drying process by microencapsulation, using a special pediatric prebiotics mixture (galactooligosaccharides and fructooligosaccharides). Probiotic microorganisms were encapsulated with a coat combination of prebiotics–calcium-alginate prior to freeze-drying. Both encapsulated and free cells were then freeze-dried in their optimized combinations of skim milk and prebiotics. Response surface methodology (RSM) was used to produce a coating combination as well as drying medium with the highest cell viability during freeze-drying. The optimum encapsulation composition was found to be 2.1 % Na-alginate, 2.9 % prebiotic, and 21.7 % glycerol. Maximum survival predicted by the model was 81.2 %. No significant (p > 0.05) difference between the predicted and experimental values verified the adequacy of final reduced models. The protection ability of encapsulation was then examined over 120 days of storage at 4 and 25 °C and exposure to a sequential model of infantile GIT conditions including both gastric conditions (pH 3.0 and 4.0, 90 min, 37 °C) and intestinal conditions (pH 7.5, 5 h, 37 °C). Significantly improved cell viability showed that microencapsulation of B. lactis 10140 with the prebiotics was successful in producing a stable symbiotic powdery nutraceutical.

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  1. Mustafa Shuhaimi

    Present address: Department of Microbiology, Faculty of Biotechnology and Biomolecular Science, University Putra Malaysia, 43400, Selangor, Malaysia

Authors and Affiliations

  1. Department of Microbiology, Faculty of Biotechnology and Biomolecular Science, University Putra Malaysia, 43400, Selangor, Malaysia

    Fatemeh Shamekhi

  2. Department of Bioprocess technology, Faculty of Biotechnology and Biomolecular Science, University Putra Malaysia, 43400, Selangor, Malaysia

    Arbakariya Ariff

  3. Department of Food Service and Management, Faculty of Food Science and Technology, University Putra Malaysia, 43400, Selangor, Malaysia

    Yazid A. Manap

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  1. Fatemeh Shamekhi
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  2. Mustafa Shuhaimi
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Correspondence to Mustafa Shuhaimi.

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Shamekhi, F., Shuhaimi, M., Ariff, A. et al. Cell viability of microencapsulated Bifidobacterium animalis subsp. lactis under freeze-drying, storage and gastrointestinal tract simulation conditions. Folia Microbiol 58, 91–101 (2013). https://doi.org/10.1007/s12223-012-0183-9

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