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Deciphering the metabolic capabilities of a lipase producing Pseudomonas aeruginosa SL-72 strain

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Abstract

Pseudomonads have been reported for their metabolic, nutritional and ecological versatility, which motivated us to prospect the metabolic profile of a lipolytic strain Pseudomonas aeruginosa SL-72. The strain SL-72 was found to produce high levels of lipase and pectinase (1,555.62 IU/mL and 1,490.33 IU/mL, respectively), esterase and amylase, besides low levels of xylanase, proteinase and cellulase. The strain also tested positive for different plant growth-promoting traits—production of ammonia, hydrogen cyanide, siderophores, phosphate solubilization, nitrate reduction and antifungal activity. The high levels of activity of aryl sulphatase, alkaline phosphatase and fluorescein diacetate hydrolase makes it a useful strain for enhanced nutrient cycling in soil. The strain SL-72 produced rhamnolipids, a biosurfactant and its production was enhanced when starch was used as carbon source (0.256 g/L) and utilized polycyclic hydrocarbon compounds viz. anthracene, phenanthrene, pyrene, fluorene and its mixture. The multifaceted nature of the culture illustrates its promise in bioremediation, industry, besides its use as an inoculant.

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Acknowledgements

The authors are thankful to Banasthali University and Division of Microbiology, Indian Agricultural Research Institute for extending the facilities for undertaking the experimental work.

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Authors and Affiliations

  1. Department of Biosciences and Biotechnology, Banasthali University, Banasthali, 304022, India

    Shikha Verma & Vinay Sharma

  2. Division of Microbiology, Indian Agricultural Research Institute, New Delhi, 110012, India

    Radha Prasanna & Lata Nain

  3. Biochemical Engineering Department, BCT Kumaon Engineering College, Dwarahat, 263653, Uttarakhand, India

    Jyoti Saxena

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  1. Shikha Verma
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Correspondence to Lata Nain.

Electronic supplementary material

Fig. 1. Screening of Extracellular enzyme activity of P. aeruginosa SL-72: (a) Lipase. (b) Esterase. (c) Xylanase. (d) Cellulase. (e) Proteinase. (f) Pectinase. (g) Amylase. (h) Acrylamidase.

Fig. 2. In vitro screening of PGP traits of lipolytic strain P. aeruginosa SL-72: (a) NH3 production. (b) Siderophore production. (c) Phosphate solubilisation. (d) HCN production. (e) Inhibition of Macrophomnia Phaseolina by P. aeruginosa SL-72.

Fig. 3. Screening of P. aeruginosa SL-72 for its biosurfactant production potential: (a) β-hemolysis. (b) Rhamnolipid production.

Fig. 4. Catechol-2,3 dioxygenase activity and PAH utilization pattern of P. aeruginosa SL-72 (a) Catechol-2,3 dioxygenase. (b) Anthracene. (c) Fluorene. (d) Phenanthrene. (e) Pyrene. (f) PAH mixture.

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Verma, S., Prasanna, R., Saxena, J. et al. Deciphering the metabolic capabilities of a lipase producing Pseudomonas aeruginosa SL-72 strain. Folia Microbiol 57, 525–531 (2012). https://doi.org/10.1007/s12223-012-0163-0

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