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Expression patterns of Ruminococcus flavefaciens 007S cellulases as revealed by zymogram approach

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Abstract

The expression of Ruminococcus flavefaciens 007S cellulases in different incubation time points (growth stages) and their substrate inducibility were analyzed by comparing the zymogram expression profiles of cultures grown on insoluble cellulose (Avicel) with cellobiose-grown cultures. The molecular weights of the enzymes were compared to (putative) cellulases encoded in the R. flavefaciens FD-1 genome.

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Abbreviations

CMC:

Carboxymethylcellulose

DTT:

1,4-Dithiothreitol

MW(s):

Molar mass(es)

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Authors and Affiliations

  1. Chair for Microbiology and Microbial Biotechnology, University of Ljubljana, Groblje 3, 1230, Domžale, Slovenia

    Maša Vodovnik & Romana Marinšek Logar

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  1. Maša Vodovnik
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  2. Romana Marinšek Logar
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Correspondence to Romana Marinšek Logar.

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Vodovnik, M., Logar, R.M. Expression patterns of Ruminococcus flavefaciens 007S cellulases as revealed by zymogram approach. Folia Microbiol 57, 367–370 (2012). https://doi.org/10.1007/s12223-012-0144-3

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  • DOI: https://doi.org/10.1007/s12223-012-0144-3

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