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Effect of recombinant divercin RV41, structural variants and the activators of potassium channels on Listeria monocytogenes EGDe

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Abstract

The effect of recombinant divercin RV41 (DvnRV41) and its structural variants on the K-channel formation was determined. The growth of Listeria monocytogenes EGDe (sensitive phenotype) and its isogenic strain (resistant phenotype) was assessed in the presence of DvnRV41 combined or not with pinacidil, NS1619, cromakalim (as K-channel activators), iberiotoxin and glipizide (as K-channel blockers). The combined action of DvnRV41 and K activators permitted formation of ATP-dependent pores. The combination of DvnRV41 and ATP-dependent pore activator cromakalim inhibited the growth of sensitive strain. The antilisterial activity of structural variants was less important than that of DvnRV41 but their mode of action remained overall similar.

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Abbreviations

CRK:

Cromakalim

GLZ:

Glipizide

IBT:

Iberiotoxin

NS1619:

A channel opener

PCD:

Pinacidil

AU:

Arbitrary units

Dvn RV41:

Recombinant divercin

LAB:

Lactic acid bacteria

LB:

Luria–Bertani (broth)

MIC:

Minimum inhibitory concentration

PMF:

Proton motive force

PTS:

Phosphotransferase system

TSB:

Tryptic soy broth

TSYEB:

TSB + yeast extract

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Acknowledgment

The authors would like to thank J. Baah for improving quality of the manuscript and the French Embassy in Prague for financial support of first author.

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Authors and Affiliations

  1. Food Science and Engineering, La Chantrerie, Nantes-Atlantic National College of Veterinary Medicine, BP 40706–44307, Nantes Cedex 03, France

    J. Rihakova, H. Prevost & D. Drider

  2. Department of Biochemistry and Microbiology, Institute of Chemical Technology, 166 28, Prague 6, Czech Republic

    J. Rihakova & K. Demnerova

Authors
  1. J. Rihakova
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  2. H. Prevost
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  3. K. Demnerova
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  4. D. Drider
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Correspondence to K. Demnerova or D. Drider.

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Rihakova, J., Prevost, H., Demnerova, K. et al. Effect of recombinant divercin RV41, structural variants and the activators of potassium channels on Listeria monocytogenes EGDe. Folia Microbiol 56, 110–115 (2011). https://doi.org/10.1007/s12223-011-0024-2

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  • DOI: https://doi.org/10.1007/s12223-011-0024-2

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