Abstract
A high-throughput, medium-discrimination method for preliminary typing and selecting non-identical isolates of lactic acid bacteria in cheeses was developed. RAMP, a PCR with one microsatellite-targeted and one random primer, was used for preliminary typing of 1119 isolates of lactic acid bacteria from Slovak Bryndza cheese. A total of 59 genotypes were identified based on RAMP profiles consisting of 12–23 DNA fragments of 150–3000 bp. For example, 18, 17, 13 and 7 different RAMP-types were identified in Lactobacillus brevis, L. plantarum, L. paracasei and L. fermentum, respectively. The method facilitated well reproducible, medium-discrimination typing of Lactobacillus spp. and Pediococcus spp. at a subspecies level and proved to be suitable for preliminary typing of lactic acid bacteria isolated from cheese.
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Abbreviations
- LAB:
-
lactic acid bacteria
- MRS:
-
De Man-Rogosa-Sharpe (agar)
- PCR:
-
polymerase chain reaction
- RAMP:
-
randomly-amplified microsatellite polymorphism
- RAPD:
-
randomly-amplified polymorphic DNA
- SPE:
-
solid phase extraction
- UPGMA:
-
unweighted pair-group method with arithmetic averages
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Chebeňová, V., Berta, G., Kuchta, T. et al. Randomly-amplified microsatellite polymorphism for preliminary typing of lactic acid bacteria from Bryndza Cheese. Folia Microbiol 55, 598–602 (2010). https://doi.org/10.1007/s12223-010-0096-4
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DOI: https://doi.org/10.1007/s12223-010-0096-4