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The Effect of Melanocyte Stimulating Hormone and Hydroxyapatite on Osteogenesis in Pulp Stem Cells of Human Teeth Transferred into Polyester Scaffolds

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Abstract

Presently, tissue engineering is employed in the restoration and repair of tissue defects. Degradable scaffolds, stem cells and stimulating factors are employed in this method. In this study, the effect of melanocyte-stimulating hormone (MSH) and/or hydroxyapatite (HA) on proliferation, osteoblast differentiation, and mineralization of human dental pulp stem cells (hDPSCs) seeded on PLLA-PCL nanofibrous scaffolds was evaluated. For this aim, PLLA-PCL-HA nanofibrous scaffolds were fabricated using electrospinning method. FE-SEM images exhibited that all nanofibers had bead-free morphologies with average diameters ranging from 150–205 nm. Human DPSCs seeded into PLLA-PCL nanofibers were treated with MSH. Cell viability, proliferation, morphology, osteogenic potential, and the expression of tissue-specific genes were assessed by means of MTT assay, FE-SEM, alizarin red S staining, and RT-PCR analysis. hDPSCs exhibited improved adhesion and proliferation capacity on the PLLA-PCL-HA nanofibers treated with MSH compared to other groups (p<0.05). Additionally, PLLA-PCL-HA nanofibers treated with MSH exhibited significantly higher mineralization and alkaline phosphatase activity than other groups. RT-PCR results confirmed that PLLA-PCL-HA nanofibers enriched with MSH could significantly unregulated the gene expression of BMP2, osteocalcin, RUNX2 and DSPP that correlated to osteogenic differentiation (p<0.05). Based on results, incorporation of HA nanoparticles in PLLA-PCL nanofibers and addition of MSH in media exhibited synergistic effects on the adhesion, proliferation, and osteogenesis differentiation of hDPSCs, and therefore assumed to be a favorable scaffold for bone tissue engineering applications.

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Authors and Affiliations

  1. Stem Cell Research Center and Oral Medicine Department of Dental Faculty, Tabriz University of Medical Sciences, Tabriz, 5166614711, Iran

    Marziyeh Aghazadeh

  2. Endodontics Department of Dental Faculty, Tabriz University of Medical Sciences, Tabriz, 5166614711, Iran

    Mohammad Samiei

  3. Department of Chemistry, University of Zanjan, Zanjan, 45195 313, Iran

    Vahideh Raeisdasteh Hokmabad

  4. Stem Cell Research Center and Department of Medical Biotechnology, Faculty of Advanced Medical Science, Tabriz University of Medical Sciences, Tabriz, 5166614711, Iran

    Effat Alizadeh

  5. Under Graduate Student of Dental Faculty, Tabriz University of Medical Sciences, Tabriz, 5166614711, Iran

    Neda Jabbari

  6. Nanotechnology and Regenerative Medicine Commercialisation Centre (NanoRegMed Ltd) The London BioScience Innovation Centre, London, NW1 0NH, United Kingdom

    Alexander Seifalian

  7. Drug Applied Research Center and Department of Medical Nanotechnology, Faculty of Advanced Medical Science, Tabriz University of Medical Sciences, Tabriz, 5166614711, Iran

    Roya Salehi

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  1. Marziyeh Aghazadeh
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  2. Mohammad Samiei
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  3. Vahideh Raeisdasteh Hokmabad
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  4. Effat Alizadeh
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  7. Roya Salehi
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Correspondence to Roya Salehi.

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Aghazadeh, M., Samiei, M., Hokmabad, V.R. et al. The Effect of Melanocyte Stimulating Hormone and Hydroxyapatite on Osteogenesis in Pulp Stem Cells of Human Teeth Transferred into Polyester Scaffolds. Fibers Polym 19, 2245–2253 (2018). https://doi.org/10.1007/s12221-018-8309-6

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  • DOI: https://doi.org/10.1007/s12221-018-8309-6

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