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Line-field confocal optical coherence tomography for three-dimensional skin imaging

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Abstract

This paper reports on the latest advances in line-field confocal optical coherence tomography (LC-OCT), a recently invented imaging technology that now allows the generation of either horizontal (x × y) section images at an adjustable depth or vertical (x × z) section images at an adjustable lateral position, as well as three-dimensional images. For both two-dimensional imaging modes, images are acquired in real-time, with real-time control of the depth and lateral positions. Three-dimensional (x × y × z) images are acquired from a stack of horizontal section images. The device is in the form of a portable probe. The handle of the probe has a button and a scroll wheel allowing the user to control the imaging modes. Using a supercontinuum laser as a broadband light source and a high numerical microscope objective, an isotropic spatial resolution of ∼1 µm is achieved. The field of view of the three-dimensional images is 1.2 mm × 0.5 mm × 0.5 mm (x × y × z). Images of skin tissues are presented to demonstrate the potential of the technology in dermatology.

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References

  1. Fercher A F. Optical coherence tomography. Journal of Biomedical Optics, 1996, 1(2): 157–173

    Article  Google Scholar 

  2. Podoleanu A G. Optical coherence tomography. Journal of Microscopy, 2012, 247(3): 209–219

    Article  Google Scholar 

  3. Zysk A M, Nguyen F T, Oldenburg A L, Marks D L, Boppart S A. Optical coherence tomography: a review of clinical development from bench to bedside. Journal of Biomedical Optics, 2007, 12(5): 051403

    Article  Google Scholar 

  4. Schuman J S, Puliafito C A, Fujimoto J G, Duker J S. Optical Coherence Tomography of Ocular Diseases. 3rd ed. New Jersey: Slack Inc., 2013

    Google Scholar 

  5. Bezerra H G, Costa M A, Guagliumi G, Rollins A M, Simon D I. Intracoronary optical coherence tomography: a comprehensive review clinical and research applications. JACC: Cardiovascular Interventions, 2009, 2(11): 1035–1046

    Google Scholar 

  6. Adler D C, Chen Y, Huber R, Schmitt J, Connolly J, Fujimoto J G. Three-dimensional endomicroscopy using optical coherence tomography. Nature Photonics, 2007, 1(12): 709–716

    Article  Google Scholar 

  7. Yu X, Ding Q, Hu C, Mu G, Deng Y, Luo Y, Yuan Z, Yu H, Liu L. Evaluating micro-optical coherence tomography as a feasible imaging tool for pancreatic disease diagnosis. IEEE Journal of Selected Topics in Quantum Electronics, 2019, 25(1): 1–8

    Article  Google Scholar 

  8. Men J, Huang Y, Solanki J, Zeng X, Alex A, Jerwick J, Zhang Z, Tanzi R E, Li A, Zhou C. Optical coherence tomography for brain imaging and developmental biology. IEEE Journal of Selected Topics in Quantum Electronics, 2016, 22(4): 120–132

    Article  Google Scholar 

  9. Fan Y, Xia Y, Zhang X, Sun Y, Tang J, Zhang L, Liao H. Optical coherence tomography for precision brain imaging, neurosurgical guidance and minimally invasive theranostics. Bioscience Trends, 2018, 12(1): 12–23

    Article  Google Scholar 

  10. Levine A, Wang K, Markowitz O. Optical coherence tomography in the diagnosis of skin cancer. Dermatologic Clinics, 2017, 35(4): 465–488

    Article  Google Scholar 

  11. Drexler W, Morgner U, Kärtner F X, Pitris C, Boppart S A, Li X D, Ippen E P, Fujimoto J G. In vivo ultrahigh-resolution optical coherence tomography. Optics Letters, 1999, 24(17): 1221–1223

    Article  Google Scholar 

  12. Povazay B, Bizheva K, Unterhuber A, Hermann B, Sattmann H, Fercher A F, Drexler W, Apolonski A, Wadsworth W J, Knight J C, Russell P S J, Vetterlein M, Scherzer E. Submicrometer axial resolution optical coherence tomography. Optics Letters, 2002, 27(20): 1800–1802

    Article  Google Scholar 

  13. Wang Y, Zhao Y, Nelson J S, Chen Z, Windeler R S. Ultrahigh-resolution optical coherence tomography by broadband continuum generation from a photonic crystal fiber. Optics Letters, 2003, 28(3): 182–184

    Article  Google Scholar 

  14. Aguirre A, Nishizawa N, Fujimoto J, Seitz W, Lederer M, Kopf D. Continuum generation in a novel photonic crystal fiber for ultrahigh resolution optical coherence tomography at 800 nm and 1300 nm. Optics Express, 2006, 14(3): 1145–1160

    Article  Google Scholar 

  15. Leitgeb R A. En face optical coherence tomography: a technology review. Biomedical Optics Express, 2019, 10(5): 2177–2201

    Article  Google Scholar 

  16. Choma M, Sarunic M, Yang C, Izatt J. Sensitivity advantage of swept source and Fourier domain optical coherence tomography. Optics Express, 2003, 11(18): 2183–2189

    Article  Google Scholar 

  17. Lee K S, Rolland J P. Bessel beam spectral-domain high-resolution optical coherence tomography with micro-optic axicon providing extended focusing range. Optics Letters, 2008, 33(15): 1696–1698

    Article  Google Scholar 

  18. Leitgeb R A, Villiger M, Bachmann A H, Steinmann L, Lasser T. Extended focus depth for Fourier domain optical coherence microscopy. Optics Letters, 2006, 31(16): 2450–2452

    Article  Google Scholar 

  19. Tamborski S, Lyu H C, Dolezyczek H, Malinowska M, Wilczynski G, Szlag D, Lasser T, Wojtkowski M, Szkulmowski M. Extended-focus optical coherence microscopy for high-resolution imaging of the murine brain. Biomedical Optics Express, 2016, 7(11): 4400–4414

    Article  Google Scholar 

  20. Liu L, Chu K K, Houser G H, Diephuis B J, Li Y, Wilsterman E J, Shastry S, Dierksen G, Birket S E, Mazur M, Byan-Parker S, Grizzle W E, Sorscher E J, Rowe S M, Tearney G J. Method for quantitative study of airway functional microanatomy using micro-optical coherence tomography. PLoS One, 2013, 8(1): e54473

    Article  Google Scholar 

  21. Liu L, Liu C, Howe W C, Sheppard C J R, Chen N. Binary-phase spatial filter for real-time swept-source optical coherence microscopy. Optics Letters, 2007, 32(16): 2375–2377

    Article  Google Scholar 

  22. Yin B, Chu K K, Liang C P, Singh K, Reddy R, Tearney G J. µOCT imaging using depth of focus extension by self-imaging wavefront division in a common-path fiber optic probe. Optics Express, 2016, 24(5): 5555–5564

    Article  Google Scholar 

  23. Ralston T S, Marks D L, Carney P S, Boppart S A. Interferometric synthetic aperture microscopy. Nature Physics, 2007, 3(2): 129–134

    Article  Google Scholar 

  24. Coquoz S, Bouwens A, Marchand P J, Extermann J, Lasser T. Interferometric synthetic aperture microscopy for extended focus optical coherence microscopy. Optics Express, 2017, 25(24): 30807–30819

    Article  Google Scholar 

  25. Yu L, Rao B, Zhang J, Su J, Wang Q, Guo S, Chen Z. Improved lateral resolution in optical coherence tomography by digital focusing using two-dimensional numerical diffraction method. Optics Express, 2007, 15(12): 7634–7641

    Article  Google Scholar 

  26. Fechtig D J, Kumar A, Drexler W, Leitgeb R A. Full range line-field parallel swept source imaging utilizing digital refocusing. Journal of Modern Optics, 2015, 62(21): 1801–1807

    Article  MathSciNet  MATH  Google Scholar 

  27. Mo J, de Groot M, de Boer J F. Focus-extension by depth-encoded synthetic aperture in optical coherence tomography. Optics Express, 2013, 21(8): 10048–10061

    Article  Google Scholar 

  28. Holmes J. Theory and applications of multi-beam OCT. In: Proceedings of the 1st Canterbury Workshop on Optical Coherence Tomography and Adaptive Optics. Canterbury: SPIE, 2008

    Google Scholar 

  29. Holmes J, Hattersley S, Stone N, Bazant-Hegemark F, Barr H. Multi-channel fourier domain OCT system with superior lateral resolution for biomedical applications. In: Proceedings of Coherence Domain Optical Methods and Optical Coherence Tomography in Biomedicine XII. San Jose: SPIE, 2008

    Google Scholar 

  30. Yi L, Sun L, Ding W. Multifocal spectral-domain optical coherence tomography based on Bessel beam for extended imaging depth. Journal of Biomedical Optics, 2017, 22(10): 1–8

    Google Scholar 

  31. Li J, Luo Y, Wang X, Wang N, Bo E, Chen S, Chen S, Chen S, Tsai M T, Liu L. Extending the depth of focus of fiber-optic optical coherence tomography using a chromatic dual-focus design. Applied Optics, 2018, 57(21): 6040–6046

    Article  Google Scholar 

  32. Nam A S, Ren J, Bouma B E, Vakoc B J. Demonstration of triband multi-focal imaging with optical coherence tomography. Applied Sciences (Basel, Switzerland), 2018, 8(12): 2395

    Google Scholar 

  33. Huber R, Wojtkowski M, Fujimoto J G, Jiang J Y, Cable A E. Three-dimensional and C-mode OCT imaging with a compact, frequency swept laser source at 1300 nm. Optics Express, 2005, 13(26): 10523–10538

    Article  Google Scholar 

  34. Rolland J P, Meemon P, Murali S, Thompson K P, Lee K S. Gabor-based fusion technique for optical coherence microscopy. Optics Express, 2010, 18(4): 3632–3642

    Article  Google Scholar 

  35. Lee K S, Thompson K P, Meemon P, Rolland J P. Cellular resolution optical coherence microscopy with high acquisition speed for in-vivo human skin volumetric imaging. Optics Letters, 2011, 36(12): 2221–2223

    Article  Google Scholar 

  36. Liu S, Mulligan J A, Adie S G. Volumetric optical coherence microscopy with a high space-bandwidth-time product enabled by hybrid adaptive optics. Biomedical Optics Express, 2018, 9(7): 3137–3152

    Article  Google Scholar 

  37. Schmitt J M, Lee S L, Yung K M. An optical coherence microscope with enhanced resolving power in thick tissue. Optics Communications, 1997, 142(4–6): 203–207

    Article  Google Scholar 

  38. Lexer F, Hitzenberger C K, Drexler W, Molebny S, Sattmann H, Sticker M, Fercher A F. Dynamic coherent focus OCT with depth-independent transversal resolution. Journal of Modern Optics, 1999, 46(3): 541–553

    Article  Google Scholar 

  39. Qi P A, Himmer P A, Gordon M L, Yang VXD, Dickensheets D L, Vitkin I A. Dynamic focus control in high-speed optical coherence tomography based on a microelectromechanical mirror. Optics Communications, 2004, 232(1–6): 123–128

    Article  Google Scholar 

  40. Divetia A, Hsieh T H, Zhang J, Chen Z, Bachman M, Li G P. Dynamically focused optical coherence tomography for endoscopic applications. Applied Physics Letters, 2005, 86(10): 103902

    Article  Google Scholar 

  41. Yang V X D, Munce N, Pekar J, Gordon M L, Lo S, Marcon N E, Wilson B C, Vitkin I A. Micromachined array tip for multifocus fiber-based optical coherence tomography. Optics Letters, 2004, 29(15): 1754–1756

    Article  Google Scholar 

  42. Dubois A, Levecq O, Azimani H, Davis A, Ogien J, Siret D, Barut A. Line-field confocal time-domain optical coherence tomography with dynamic focusing. Optics Express, 2018, 26(26): 33534–33542

    Article  Google Scholar 

  43. Dubois A, Levecq O, Azimani H, Siret D, Barut A, Suppa M, Del Marmol V, Malvehy J, Cinotti E, Rubegni P, Perrot J L. Line-field confocal optical coherence tomography for high-resolution non-invasive imaging of skin tumors. Journal of Biomedical Optics, 2018, 23(10): 1–9

    Article  Google Scholar 

  44. Ogien J, Siret D, Levecq O, Azimani H, David A, Xue W, Perrot J L, Dubois A. Line-field confocal optical coherence tomography. In: Proceedings of Optical Coherence Tomography and Coherence Domain Optical Methods in Biomedicine XXIII. San Francisco: SPIE, 2019

    Google Scholar 

  45. Davis A, Levecq O, Azimani H, Siret D, Dubois A. Simultaneous dual-band line-field confocal optical coherence tomography: application to skin imaging. Biomedical Optics Express, 2019, 10(2): 694–706

    Article  Google Scholar 

  46. Dubois A, Xue W, Levecq O, Bulkin P, Coutrot A L, Ogien J. Mirau-based line-field confocal optical coherence tomography. Optics Express, 2020, 28(6): 7918–7927

    Article  Google Scholar 

  47. Ogien J, Levecq O, Azimani H, Dubois A. Dual-mode line-field confocal optical coherence tomography for ultrahigh-resolution vertical and horizontal section imaging of human skin in vivo. Biomedical Optics Express, 2020, 11(3): 1327–1335

    Article  Google Scholar 

  48. Larkin K G. Efficient nonlinear algorithm for envelope detection in white light interferometry. Journal of the Optical Society of America A, Optics, Image Science, and Vision, 1996, 13(4): 832–843

    Article  Google Scholar 

  49. Cazalas M, Levecq O, Azimani H, Siret D, Barut A, Suppa M, del Marmol V, Malvehy J, Cinotti E, Rubegni P, Perrot J L, Dubois A. Skin lesion imaging with line-field confocal optical coherence tomography. In: Proceedings of Photonics in Dermatology and Plastic Surgery 2019. San Francisco: SPIE, 2019

    Google Scholar 

  50. Dejonckheere G, Suppa M, Marmol V, Meyer T, Stockfleth E. The actinic dysplasia syndrome-diagnostic approaches defining a new concept in field carcinogenesis with multiple cSCC. Journal of the European Academy of Dermatology and Venereology, 2019, 33(S8): 16–20

    Article  Google Scholar 

  51. Pedrazzani M, Breugnot J, Rouaud-Tinguely P, Cazalas M, Davis A, Bordes S, Dubois A, Closs B. Comparison of line-field confocal optical coherence tomography images with histological sections: validation of a new method for in vivo and non-invasive quantification of superficial dermis thickness. Skin Research and Technology, 2020, 26(3): 398–404

    Article  Google Scholar 

  52. Ogien J, Levecq O, Cazalas M, Suppa M, del Marmol V, Malvehy J, Cinotti E, Rubegni P, Perrot J L, Dubois A. Handheld line-field confocal optical coherence tomography for dermatology. In: Proceedings of Photonics in Dermatology and Plastic Surgery 2020. San Francisco: SPIE, 2020

    Google Scholar 

  53. Monnier J, Tognetti L, Miyamoto M, Suppa M, Cinotti E, Fontaine M, Perez J, Orte Cano C, Yélamos O, Puig S, Dubois A, Rubegni P, Marmol V, Malvehy J, Perrot J L. In vivo characterization of healthy human skin with a novel, non-invasive imging technique: line-field confocal optical coherence tomography. Journal of the European Academy of Dermatology and Venereology, 2020, doi:https://doi.org/10.1111/jdv.16857

  54. Ruini C, Sattler E. Line-field confocal optical coherence tomography: the golden goose? Aktuelle Dermatologie, 2020, 46: 148–151

    Article  Google Scholar 

  55. Tognetti L, Rizzo A, Fiorani D, Cinotti E, Perrot J L, Rubegni P. New findings in non-invasive imaging of aquagenic keratoderma: Line-field optical coherence tomography, dermoscopy and reflectance confocal microscopy. Skin Research and Technology, 2020, doi:https://doi.org/10.1111/srt.12882

  56. Ruini C, Schuh S, Sattler E, Welzel J. Line-field confocal optical coherence tomography-practical applications in dermatology and comparison with established imaging methods. Skin Research and Technology, 2020, doi:https://doi.org/10.1111/srt.12949

  57. Tognetti L, Fiorani D, Cinotti E, Rubegni P. Tridimensional skin imaging in aquagenic keratoderma: virtual histology by line-field confocal optical coherence tomography. International Journal of Dermatology, 2020, doi:https://doi.org/10.1111/ijd.15169

  58. Tognetti L, Fiorani D, Suppa M, Cinotti E, Fontaine M, Marmol V D, Rubegni P, Perrot J L. Examination of circumscribed palmar hypokeratosis with line-field confocal optical coherence tomography: dermoscopic, ultrasonographic and histopathologic correlates. Indian Journal of Dermatology, Venereology and Leprology, 2020, 86(2): 206–208

    Article  Google Scholar 

  59. Tognetti L, Carraro A, Lamberti A, Cinotti E, Suppa M, Luc Perrot J, Rubegni P. Kaposi sarcoma of the glans: new findings by line field confocal optical coherence tomography examination. Skin Research and Technology, 2020, doi:https://doi.org/10.1111/srt.12938

  60. Ruini C, Schuh S, Pellacani G, French L, Welzel J, Sattler E. In vivo imaging of Sarcoptes scabiei infestation using line-field confocal optical coherence tomography. Journal of the European Academy of Dermatology and Venereology, 2020, doi:https://doi.org/10.1111/jdv.16671

  61. Rennie D. Nailfold dermatoscopy in general practice. Australian Family Physician, 2015, 44(11): 809–812

    Google Scholar 

  62. Xue W, Ogien J, Levecq O, Dubois A. Line-field confocal optical coherence tomography based on a Mirau interferometer. In: Proceedings of Unconventional Optical Imaging II. SPIE, 2020

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Acknowledgements

The authors thank the whole team of engineers at DAMAE Medical, especially Olivier Levecq, Hicham Azimani, Emmanuel Cohen and Romain Allemand, for their work on the technology and design of the LC-OCT prototype presented in this paper. They also thank Amyn Kassara for providing the segmentation of the three-dimensional images. They are also grateful to Anaïs Barut and David Siret as directors and managers of DAMAE Medical.

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Authors and Affiliations

  1. DAMAE Medical, Paris, 75013, France

    Jonas Ogien, Anthony Daures & Maxime Cazalas

  2. CHU St-Etienne, Service Dermatologie, Saint-Etienne, 42055, France

    Jean-Luc Perrot

  3. Université Paris-Saclay, Institut d’Optique Graduate School, CNRS, Laboratoire Charles Fabry, Palaiseau, 91127, France

    Arnaud Dubois

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  1. Jonas Ogien
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  2. Anthony Daures
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  3. Maxime Cazalas
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  4. Jean-Luc Perrot
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  5. Arnaud Dubois
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Correspondence to Arnaud Dubois.

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Jonas Ogien received his M.S. degree in Optics in 2014 from University of Rochester (USA) and his Ph.D. degree in Physics in 2017 from Paris-Saclay University (France). He also holds an engineering degree from Institut d’Optique Graduate School (France) and currently works as a research engineer at DAMAE Medical, a startup company working on OCT for skin imaging. He is particularly interested in innovative optical methods for high-resolution imaging. His current research focuses on the development of new modalities and optical improvements in OCT.

Anthony Daures received his engineering degree in Physics from Institut National des Sciences Appliquées in Toulouse (France) in 2014. He also holds an M.S. degree in Medical Imaging from University of Paul Sabatier (Toulouse, France). As an image processing engineer with more than seven years of experience, he has worked for different medical imaging companies and has always focused on the development of innovative algorithms. He is currently working as an image quality engineer at DAMAE Medical to improve diagnosis in real-time.

Maxime Cazalas, M.Sc., is a computer science and image processing engineer with over 14 years of experience in medical imaging. Passionate about innovation in the field of medical imaging, he has been involved in the development of new and innovative imaging solutions to assist physicians in delivering the best possible care to their patient. Maxime is currently focused on dermato-oncology, using in vivo optical imaging technics to better understand the pathogenesis of skin tumors, enabling earlier diagnosis and optimal patient-tailored therapy.

Jean-Luc Perrot, Ph.D. & M.D., has a 30-year career as a dermatologist and medical oncologist at Saint-Etienne University Hospital (France). He was the former president of the non-invasive skin imaging group of the French Society of Dermatology. Multimodal skin imaging has developed considerably in his dermatology department where different optical techniques are combined, including confocal microscopy, OCT, LC-OCT, dermatoscopy and Raman spectroscopy. Perrot has been involved in the validation and/or design of several innovative imaging devices. Although he has published numerous books on skin imaging, his primary objective remains the management of patients in the context of daily practice.

Arnaud Dubois received his Ph.D. degree in Physics in 1997 from Paris-Saclay University (France). Since 2006, he is a professor of optics at Université Paris-Saclay, Institut d’Optique Graduate School (France). Pioneer in full-field OCT in the early 2000, Dubois has since been a major contributor to the development of this technology. He has published 120 research articles in scientific journals and conference proceedings and 12 book chapters. He was the scientific editor in 2016 of the first and only handbook entirely devoted to full-field OCT. In 2014, Dubois co-founded DAMAE Medical, a startup company working on an innovative OCT technique for skin imaging.

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Ogien, J., Daures, A., Cazalas, M. et al. Line-field confocal optical coherence tomography for three-dimensional skin imaging. Front. Optoelectron. 13, 381–392 (2020). https://doi.org/10.1007/s12200-020-1096-x

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