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Prediction model for CD34 positive cell yield in peripheral blood stem cell collection on the fourth day after G-CSF administration in healthy donors

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Abstract

Allogeneic peripheral blood stem cell transplantation (PBSCT) is an indispensable treatment option for hematological malignancy. The optimal collection day after granulocyte colony-stimulating factor (G-CSF) administration should be determined by peripheral blood pre-apheresis CD34 positive (CD34+) cell percentage. However, pre-apheresis CD34+ cell analysis is not available for most institutions in Japan. Prediction of the optimal collection day based on objective parameters, other than direct CD34+ cell count, is thus an important matter for investigation. To identify potential predictive factors, clinical parameters in 79 related donors who received allogeneic peripheral blood stem cell (PBSC) collection were analyzed. Eight factors were significantly correlated with the number of CD34+ cells per donor body weight on the fourth day (day 4) after G-CSF administration in univariate analysis. Using multi-regression analysis, we made a simple scoring system comprising age, sex, LDH on day 4 and RBC count at the baseline, which significantly predicted CD34+ cell yield (P = 0.048). This system allows us to determine the optimal PBSC collection day. When the score is 0 or 1 on day 4, starting apheresis on day 5 potentially helps avoiding the need for multiple harvests. Score 3 or 4 on day 4 is indicative of better performance if apheresis is started on day 4.

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Acknowledgments

We are grateful for the technical assistance of all the staff of the Department of Transfusion Medicine and the Department of Clinical Laboratory, in especial to Ms. Mika Matsuhashi, Ms. Yuuko Mishima, Mr. Masahiro Johna, and Mr. Naoyuki Yoshikawa.

Conflict of interest

The authors declare that they have no conflicts of interest relevant to the manuscript.

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Authors and Affiliations

  1. Department of Transfusion Medicine, Graduate School of Medicine, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo, 113-8655, Japan

    Tetsuichi Yoshizato, Naoko Watanabe-Okochi, Tomohiko Sato, Nelson Hirokazu Tsuno & Koki Takahashi

  2. Department of Hematology and Oncology, Graduate School of Medicine, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo, Japan

    Yasuhito Nannya, Motoshi Ichikawa, Tsuyoshi Takahashi & Mineo Kurokawa

  3. Department of Clinical Laboratory Medicine, Graduate School of Medicine, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo, Japan

    Akiko Masuda & Yutaka Yatomi

  4. Department of Cell Therapy and Transplantation Medicine, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo, Japan

    Mineo Kurokawa

Authors
  1. Tetsuichi Yoshizato
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  2. Naoko Watanabe-Okochi
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  3. Yasuhito Nannya
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  4. Motoshi Ichikawa
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  5. Tsuyoshi Takahashi
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  8. Yutaka Yatomi
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  9. Nelson Hirokazu Tsuno
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  10. Mineo Kurokawa
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Corresponding author

Correspondence to Nelson Hirokazu Tsuno.

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Yoshizato, T., Watanabe-Okochi, N., Nannya, Y. et al. Prediction model for CD34 positive cell yield in peripheral blood stem cell collection on the fourth day after G-CSF administration in healthy donors. Int J Hematol 98, 56–65 (2013). https://doi.org/10.1007/s12185-013-1366-0

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  • DOI: https://doi.org/10.1007/s12185-013-1366-0

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